THE DEVELOPMENT OF METHOD OF QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN THE LEAVES OF MYRTUS COMMUNIS
UDC 615.322: 547.972 + 543.544
Abstract
Myrtle (Myrtus communis L.) is an evergreen plant, a small tree or shrub endemic to the Mediterranean region. In the area of growth since ancient times, various parts (berries, fruits and leaves) of myrtle are used in folk medicine. The therapeutic properties of varies parts of the myrtle can be caused with different biologically active compounds, such as terpenoids (α-pinene, 1,8-cineole, geranyl acetate, linalool, limonene, trans-myrtanol acetate), polyphenolic compounds named flavonoids (quercetin, myricetin, myrtocommulones etc. At present time the myrtle is successfully cultivated in the coastal zone of the southern coast of Crimea. We studied samples of leaves of myrtle, harvested in the Nikitsky Botanical Garden – National Science Center of the Russian Academy of Sciences. The optimal conditions for the extraction of flavonoids from the leaves of were determined. It was established that the appropriate extragent for this raw material is 80% ethanol. The extraction time is 90 min, the ratio of "raw material-extragent" is 1 : 50. The method has been developed for the quantitative determination of the total flavonoids (calculated on rutin) in the leaves of myrtle by the method of differential spectrophotometry, at an analytical wavelength of 414 nm. Using the developed methodic, a number of samples of the myrtle leaves were analyzed, and it was established that the content of the total flavonoids in the leaves of myrtle varies from 2.48±0.08% to 3.64±0.12% (calculated on rutin) .
Downloads
Metrics
References
Aleksic V., Knezevic P. Microbiological Research, 2014, vol. 169, no. 4, pp. 240–254.
Hennia A., Miguel M.G., Nemmiche S. Medicines, 2018, vol. 5, no. 3, p. 89.
Migliore J., Baumel A., Juin M., Médail F. Journal of Biogeography, 2012, no. 39, pp. 942–956.
Yoshimura M., Amakura Y., Tokuhara M., Yoshida T. Journal of Natural Medicines, 2008, vol. 62, no. 3, pp. 366–368.
Alipour G., Dashti S., Hosseinzadeh H. Phototherapy Research, 2014, vol. 28, no. 8, pp. 1125–1136.
Bakova Ye.Yu., Paliy A.Ye., Bakova N.N., Fedotova I.A. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2018, vol. 21 (11), pp. 30–35. (in Russ.).
Chalchat J.-C., Garry R.-P., Michet A. Journal of Essential Oil Research, 1998, vol. 10, pp. 613–617.
Flamin G., Cioni P.L., Morelli I., Maccioni S., Baldini R. Food Chemistry, 2004, vol. 85, no. 4, pp. 599–604.
Bakova N.N., Bakova Ye.Yu., Paliy A.Ye, Konovalov D.A. Biotekhnologiya kak instrument sokhraneniya biora-znoobraziya rastitel'nogo mira (fiziologo-biokhimicheskiye, embriologicheskiye, geneticheskiye i pravovyye aspekty). Materialy VIII Mezhdunarodnoy nauchno-prakticheskoy konferentsii. [Biotechnology as a tool for preserving the biodi-versity of the plant world (physiological, biochemical, embryological, genetic and legal aspects). Materials of the VIII International Scientific and Practical Conference]. 2018, pp. 121–122. (in Russ.).
Feißt C., Franke L., Appendino G., Werz O. The Journal of Pharmacology and Experimental Therapeutics, 2005, vol. 315, no. 1, pp. 389–396.
Gerbeth K., Hüsch J., Meins J., Rossi A., Sautebin L., Wiechmann K., Werz O., Skarke C., Barrett J.S., Schubert-Zsilavecz M., Abdel-Tawab M. Planta Med., 2012, no. 78, pp. 1932–1938.
Gerbeth K., Meins J., Werz O., Schubert-Zsilavecz M., Abdel-Tawab M. Planta Med., 2011, no. 77, pp. 450–454.
Hans M., Charpentier M., Huch V., Jauch J., Bruhn T., Bringmann G., Quandt D. Journal Natural Products, 2015, no. 78, pp. 2381−2389.
Koeberle A., Pollastro F., Northoff H., Werz O. British Journal of Pharmacology, 2009, no. 156, pp. 952–961.
Nicoletti R., Ferranti P., Caira S., Misso G., Castellano M., Di Lorenzo G., Caraglia M. World Journal Microbiol Bio-technol, 2014, no. 30, pp. 1047–1052.
Pharmacopee Francaise XI edition. pp. 2012–2015.
Gosudarstvennyy reyestr lekarstvennykh sredstv [State register of medicines]. URL: https://grls.rosminzdrav.ru/Default.aspx (Date of treatment 09/02/2019). (in Russ.).
Gosudarstvennaya Farmakopeya Rossiyskoy Federatsii. 14 izd. [State Pharmacopoeia of the Russian Federation. 14th ed.]. Moscow, 2018. URL: http://femb.ru/femb/pharmacopea.php. (in Russ.).
Kurkin V.A. Osnovy fitoterapii: uchebnoye posobiye. [Fundamentals of herbal medicine: a tutorial]. Samara, 2009, 963 p. (in Russ.).
Kurkin V.A. Farmakognoziya. Uchebnik dlya studentov farmatsevticheskikh vuzov (fakul'tetov). [Pharmacognosy. Textbook for students of pharmaceutical universities (faculties)]. Samara, 2019, 1278 p. (in Russ.).
Kurkin V.A., Kurkina A.V., Avdeyeva Ye.V. Fundamental'nyye issledovaniya, 2013, no. 11-9, pp. 1897–1901. (in Russ.).
Kurkina A.V. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2013, no. 5, p. 38. (in Russ.).
Kurkina A.V. Fenol'nyye soyedineniya: fundamental'nyye i prikladnyye aspekty: materialy dokladov VIII Mezhduna-rodnogo simpoziuma. [Phenolic compounds: fundamental and applied aspects: materials of reports of the VIII Interna-tional symposium]. Moscow, 2012, pp. 571–577. (in Russ.).
Kurkina A.V. Flavonoidy farmakopeynykh rasteniy: monografiya. [Flavonoids of pharmacopoeial plants: monograph]. Samara, 2012, 290 p. (in Russ.).
Kurkina A.V. Eksperimental'no-teoreticheskoye obosnovaniye podkhodov k standartizatsii syr'ya i preparatov farmakopeynykh rasteniy, soderzhashchikh flavonoidy: avtoref. diss. … d. farm. nauk. [Experimental and theoretical substantiation of approaches to standardization of raw materials and preparations of pharmacopoeial plants containing flavonoids: author. diss. ... d. Farm. sciences]. Samara, 2012, 49 p. (in Russ.).
Kurkin V.A., Avdeeva E.V., Kurkina A.V. et al. Drug Discovery and Therapy World Congress, Boston; USA, 2013, p. 170.
Kurkin V.A., Zaytseva Ye.N., Pravdivtseva O.Ye., Kurkina A.V., Stenyayeva V.V. Nauka i innovatsii v meditsine, 2019, vol. 4, no. 3, pp. 69–72. (in Russ.).
Kupriyanova Ye.A., Kurkin V.A. Aspirantskiy vestnik Povolzh'ya, 2018, no. 5–6, pp. 17–21. (in Russ.).
Copyright (c) 2021 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
