INFLUENCE OF POLYSACCHARIDE COMPLEXES OF PLANTS OF CENTRAL RUSSIA ON THE P-GLYCOPROTEIN ACTIVITY IN VITRO
UDC 615.322:547.458]015.42
Abstract
The purpose of this work was to study the effect of polysaccharide complexes isolated from tansy flowers (Tanacetum vulgare L., fam. Asteraceae) and melilotus herb (Melilotus officinalis L., fam. Fabaceae) on P-glycoprotein (Pgp, ABCB1 protein) activity in vitro.
On Caco-2 cell line the effect of polysaccharide complex isolated from tansy flowers and melilotus herb on Pgp activity was studied. In vitro Pgp activity was assessed by the transport of fexofenadine in the transwell system. High performance liquid chromatography with UV detection at wavelength 220 nm was used to determine fexofenadine concentration in the transport medium.
It was revealed that when polysaccharide isolated from tansy flowers was added to the transport medium in concentrations 10 and 100 μM the ratio of the apparent permeability coefficients of fexofenadine b-a/a-b decreased by 1.81 and 2.65 times, respectively, compared with the series of isolated transport of fexofenadine, which indicated decreased Pgp functional activity under the polysaccharide action. The polysaccharide complex of the melilotus herb did not change the b-a/a-b ratio in any of the applied concentrations, thus it did not affect the activity of this transporter. It is advisable to continue the study of tansy flower polysaccharide complex as an inhibitor of Pgp transporter protein in order to assess the possibility of its clinical use for the treatment of pharmacoresistant forms of cancer by overcoming the phenomenon of multidrug resistance of cells.
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Krishtanova N.A., Safonova M.Yu., Bolotova V.Ts., Pavlova E.D., Sakanyan E.I. Vestnik VGU. Seriya: Khimiya. Biologiya. Farmatsiya, 2005, no. 1, pp. 212–221. (in Russ.).
Chernykh I.V., Kirichenko Ye.Ye., Shchul'kin A.V., Popova N.M., Kotlyarova A.A., Yakusheva Ye.N. Ross. med.-biol. vestn. im. akad. I.P. Pavlova, 2018, vol. 26, no. 2, pp. 305–316. (in Russ.).
Chen Y., Zhang K., Li Y., Guo R., Zhang K., Zhong G., He Q. J. Cell Mol. Med., 2019, vol. 23(3), pp. 2115–2124. DOI: 10.1111/jcmm.14123.
Mollazadeh S., Sahebkar A., Hadizadeh F., Behravan J., Arabzadeh S. Life Sci., 2018, vol. 214, pp. 118–123. DOI: 10.1016/j.lfs.2018.10.048.
O’Connor R., Ooi M.G., Meiller J., Jakubikova J., Klippel S., Delmore J., Richardson P., Anderson K., Clynes M., Mitsiades C.S., O’Gorman P. Cancer Chemother. Pharmacol., 2013, vol. 71, no. 5, pp. 1357–1368.
Dalton W.S., Grogan T.M., Meltzer P.S., Scheper R.J., Durie B.G., Taylor C.W., Miller T.P., Salmon S.E. J. of clinical oncology, 1989, no. 7(4), pp. 415–424. DOI: 10.1200/JCO.1989.7.4.415.
Luk F., Bebawy M., Callaghan R. Drug Metabolism and Disposition, 2014, no. 42(4), pp. 623–631. DOI: 10.1124/dmd.113.056176.
Chernykh I.V., Shchul'kin A.V., Yakusheva Ye.N., Kirichenko Ye.Ye., Popova N.M., Yesenina A.S., Gradinar' M.M. Eruditio Juvenium, 2019, vol. 7, no. 3, pp. 349–357. (in Russ.).
Bocci G., Moreau A., Vayer P., Denizot C., Fardel O., Parmentier Y. Europ. J. Pharmac. Sci., 2018, vol. 121, pp. 85–94. DOI: 10.1016/j.ejps.2018.04.039.
Yengalycheva Ye.Ye., Yakusheva Ye.N., Sychev I.A., Shchul'kin A.V. Ross. med.-biol. vestn. im. akad. I.P. Pavlova, 2015, no. 2, pp. 50−55. (in Russ.).
Vasina T.M., Mykots L.P., Stepanova N.N., Zyablitseva N.S., Belousova A.L., Kompantsev V.A. Sibirskiy med. Zhurnal, 2012, vol. 3, pp. 126–128. (in Russ.).
Nikulin A.V., Yamshchikova S.A., Potanina O.G., Abramovich R.A. Biofarmatsevticheskiy zhurnal, 2018, no. 10(5), pp. 42–59. (in Russ.).
Yakusheva Ye.N., Shchul'kin A.V., Chernykh I.V., Popova N.M., Kotlyarova A.A., Slepnev A.A. Obzory po klinicheskoy farmakologii i lekarstvennoy terapii, 2019, vol. 17, no. 1, pp. 71–78. (in Russ.).
Petri N., Tannergren C., Rungstad D., Lennernäs H. Pharmac. Res., 2004, vol. 21(8), pp. 1398–1404. DOI: 10.1023/B:PHAM.0000036913.90332.b1.
Elsby R., Surry D.D., Smith V.N., Gray A.J. Xenobiotica, 2008, vol. 38(7–8), pp. 1140–1164. DOI: 10.1080/00498250802050880.
Garrigos M., Mir L.M., Orlowski S. Eur. J. Biochem., 1997, vol. 244(2), pp. 664–673. DOI: 10.1111/j.1432-1033.1997.00664.x.
Kryzhanovskiy S.P., Bogdanovich L.N., Besednova N.N. Ivanushko L.A., Golovacheva V.D. Fundamental'nyye issle-dovaniya, 2014, no. 10, pp. 93–100. (in Russ.).
Zlobin A.A., Martinson Ye.A., Ovechkina I.A., Durnev Ye.A., Ovodova R.G., Litvinets S.G. Khimiya Rastitel'nogo Syr'ya, 2011, no. 1, pp. 33–38. (in Russ.).
Cheng J.W., Zhang L.J., Hou Y.Q., Zhao Q., Zhang X.-J., Chen Z.-F., Bai Y. Epilepsy Behavior, 2014, no. 36, pp. 173−179. DOI: 10.1016/j.yebeh.2014.05.007.
Slomiany M.G., Grass G.D., Robertson A.D., Yang X.Y., Maria B.L., Beeson C., Toole B.P. Cell, Tumor, and Stem Cell Biology, 2009, vol. 69(4), pp. 1293–1301. DOI: 10.1158/0008-5472.CAN-08-2491.
Fenyvesi F., Fenyvesi É., Szente L., Goda K., Bacsó Z., Bácskay I., Váradi J., Kiss T., Molnár É., Janáky T., Szabó G.Jr, Vecsernyés M. European Journal of Pharmaceutical Sciences, 2008. Vol. 34(4–5), pp. 236–242. DOI: 10.1016/j.ejps.2008.04.005.
Cruz-Morales S., Castaneda-Gomez J., Rosas-Ramirez D., Fragoso-Serrano M., Figueroa-González G., Lorence A., Pereda-Miranda R. J. of Nat. Prod., 2016, vol. 79(12), pp. 3093–3104. DOI: 10.1021/acs.jnatprod.6b00782.
Kurkina A.V. Khimiya Rastitel'nogo Syr'ya, 2011, no. 4, pp. 209–212. (in Russ.).
Yakusheva Ye.N., Sychev I.A., Kirichenko Ye.Ye., Shchul'kin A.V. Fundamental'nyye issledovaniya, 2014, no. 7, pp. 1070–1074. (in Russ.).
Bubenchikova V.N., Drozdova I.L. Khimiko-farmatsevticheskiy zhurnal, 2004, vol. 38, no. 4, pp. 24–25. (in Russ.).
Fedoseyeva L.M., Kharlampovich T.A. Khimiya Rastitel'nogo Syr'ya, 2013, no. 2, pp. 153−157. (in Russ.).
Seelig A., Landwojtowicz E. European Journal of Pharmaceutical Sciences, 2000, vol. 12(1), pp. 31–40. DOI: 10.1016/S0928-0987(00)00177-9.
Polle A.YA., Ovodova R.G., Popov S.V. Khimiya Rastitel'nogo Syr'ya, 1999, no. 1, pp. 33−38. (in Russ.).
Fedoseyeva L.M., Kharlampovich T.A. Novyye dostizheniya v khimii i khimicheskoy tekhnologii rastitel'nogo syr'ya: materialy VI Vserossiyskoy konferentsii s mezhdunarodnym uchastiyem. [New achievements in the chemistry and chemical technology of plant raw materials: materials of the VI All-Russian conference with international participation]. Barnaul, 2014, pp. 275–277. (in Russ.).
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