ISOLATION AND IDENTIFICATION BETACYANINS PIGMENTS FROM BETA VULGARIS AND AMARANTHUS RETROFLEXUS
Abstract
This paper presents a new method of isolation betacyanins pigments from Beta vulgaris and Amaranthus retroflexus. Isolation was performed on a preparative chromatography column with the «gravity» elution with further identification of the obtained red-purple pigments in the infrared and visible spectral region. Established that the obtained red-purple pigments have a maximum absorption in the visible region of the spectrum at 532 nm is the characteristic wavelength for betacyanins pigments. Analysis of the IR spectra of the studied samples revealed that the red-purple powders are betacyanins pigments of characteristic absorption bands. The data obtained indicate that the isolated red-purple pigments belong to a class betacyanins for analysis on a spectrophotometer and infrared spectrometer. Thus, the presented method can obtain a red-purple powders containing betacyanins pigments which can be used as dietary supplements have been as used method for obtaining are not applicable acids, salts and other substances, with the exception of water and alcohol.
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