ISOLATION AND IDENTIFICATION BETACYANINS PIGMENTS FROM BETA VULGARIS AND AMARANTHUS RETROFLEXUS

  • Игорь (Igor') Витальевич (Vital'evich) Слепцов (Slepcov) Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980 Email: neroxasg@mail.ru
  • Иван (Ivan) Васильевич (Vasil'evich) Воронов (Voronov) Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980 Email: viv_2002@mail.ru
  • Алла (Alla) Николаевна (Nikolaevna) Журавская (Zhuravskaja) Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980 Email: jan43@mail.ru
  • Елена (Elena) Рудольфовна (Rudol'fovna) Поскачина (Poskachina) Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980 Email: poskachinalena@yandex.ru
Keywords: Beta vulgaris, Amaranthus retroflexus, betacyanins, amaranthine, betanin, infrared spectroscopy, visible spectrophotometry

Abstract

This paper presents a new method of isolation betacyanins pigments from Beta vulgaris and Amaranthus retroflexus. Isolation was performed on a preparative chromatography column with the «gravity» elution with further identification of the obtained red-purple pigments in the infrared and visible spectral region. Established that the obtained red-purple pigments have a maximum absorption in the visible region of the spectrum at 532 nm is the characteristic wavelength for betacyanins pigments. Analysis of the IR spectra of the studied samples revealed that the red-purple powders are betacyanins pigments of characteristic absorption bands. The data obtained indicate that the isolated red-purple pigments belong to a class betacyanins for analysis on a spectrophotometer and infrared spectrometer. Thus, the presented method can obtain a red-purple powders containing betacyanins pigments which can be used as dietary supplements have been as used method for obtaining are not applicable acids, salts and other substances, with the exception of water and alcohol.

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Author Biographies

Игорь (Igor') Витальевич (Vital'evich) Слепцов (Slepcov), Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980

аспирант, тел. (4112) 33-55-79

Иван (Ivan) Васильевич (Vasil'evich) Воронов (Voronov), Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980

научный сотрудник, кандидат биологических наук, тел. (4112) 33-55-79

Алла (Alla) Николаевна (Nikolaevna) Журавская (Zhuravskaja), Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980
главный научный сотрудник, доктор биологических наук, профессор, тел.: (4112) 33-55-79
Елена (Elena) Рудольфовна (Rudol'fovna) Поскачина (Poskachina), Institute of Biological Problems of the permafrost zone, Siberian Branch of the Russian Academy of Sciences, Lenina st., 41, Yakutsk, 677980
научный сотрудник, кандидат биологических наук, тел. (4112) 33-55-79

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Published
2015-07-24
How to Cite
1. Слепцов (Slepcov)И. (Igor’) В. (Vital’evich), Воронов (Voronov)И. (Ivan) В. (Vasil’evich), Журавская (Zhuravskaja)А. (Alla) Н. (Nikolaevna), Поскачина (Poskachina)Е. (Elena) Р. (Rudol’fovna) ISOLATION AND IDENTIFICATION BETACYANINS PIGMENTS FROM BETA VULGARIS AND AMARANTHUS RETROFLEXUS // chemistry of plant raw material, 2015. № 3. P. 111-115. URL: http://journal.asu.ru/cw/article/view/757.
Section
Low-molecular weight compounds