DEVELOPMENT OF THE APPROACH FOR DETERMINATION OF SCHIZANDROL A AND B IN THE EXTRACTS FROM SEEDS OF SCHISANDRA CHINENSIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH TANDEM MASS-SPECTROMETRIC DETECTION
Abstract
An approach of simultaneous detection of schizandrol A and B in extracts from plant material was developed on the basis of liquid chromatography/mass spectrometry (limits of detection were 8 and 4 ng/ml for schizandrol A and B respectively). Appropriate conditions of electrospray ionization in positive ion and tandem mass-spectrometric detection in multiple reaction monitoring mode have been chosen. Analysis of extracts was carried out using a reversed-phase chromatography in gradient elution mode with C18 sorbent. The proposed approach was tested during the analysis of water infusion and water-alcoholic extract from the seeds of Schisandra chinensis.
It is shown that in the preparation of the traditional means – water infusion the extracted amount of the analytes was 9 times lower than that of the extraction with water-alcohol mixture. Developed method has a higher selectivity in comparison with the approaches described in the literature where UV detection is used for schizandrol A and B and similar compounds detection, and hence allows the determination of these compounds, even in case of incomplete chromatographic separation with the other components of the sample.
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Liu H., Lai H., Jia X., Liu J., Zhang Z., Qi Y., Zhang J., Song J., Wu C., Zhang B., Xiao P. Comprehensive chemical analysis of Schisandra chinensis by HPLC–DAD–MScombined with chemometrics // Phytomedicine. 2013. Vol. 20, N12. Pp. 1135–1143.
Chinese Pharmacopoeia Commission, 2010. Pharmacopoeia of the People’s Republic of China Version. Chin. Med. Sci. Press, Beijing. 2010.
Song Yu C., Fei L. Clinical Guide to Chinese Herbs and Formulae. Churchill Livingstone, London, 1993. 304 p.
Zhang W.D., Wang Q., Wang Y., Wang X.J., Pu J.X., Gu Y., Wang R. Application of ultrahigh-performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis // Journal of Separation Science. 2012. Vol. 35, N17. Pp. 2203–2209.
Peng J., Fan G., Qu L., Zhou X., Wu Y. Application of preparative high-speed counter-current chromatography for iso-lation and separation of schizandrin and gomisin A from Schisandra chinensis // Journal of Chromatography A. 2005. Vol. 1082, N2. Pp. 203–207.
Li L.Y., Liu Y., Li W. Determination of schisantherin A and schiandrin A in Fructus schisandrae sphenantherae softcapsules by HPLC // Anhui Medical and Pharmaceutical Journal. 2011. Vol. 2. Pp. 158–160.
Wei H., Sun L.N., Tai Z.G., Gao S.H., Xu W., Chen W.S. A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera // Analytica Chimica Acta. 2010. Vol. 662, N1. Pp. 97–104.
Yuan G., Liu Y., Li T., Wang Y., Sheng Y., Guan M. Simultaneous and Rapid Determination of Main Lignans in Dif-ferent Parts of Schisandra Sphenanthera by Micellar Electrokinetic Capillary Chromatography // Molecules 2011. Vol. 16, N5. Pp. 3713–3722.
Wei B., Li Q., Su D., Fan R., Zhao L., Geng L., He B., Chen X., Jia Y., Bi K. Development of a UFLC–MS/MS meth-od for simultaneous determination of six lignans of Schisandra chinensis (Turcz.) Baill. in rat plasma and its application to a comparative pharmacokinetic study in normal and insomnic rats // Journal of Pharmaceutical and Biomedical Analy-sis. 2013. Vol. 77. Pp. 120–127.
Xu M., Wang G., Xie H., Huang Q., Wang W., Jia Y. Pharmacokinetic comparisons of schizandrin after oral admin-istration of schizandrin monomer, Fructus Schisandrae aqueous extract and Sheng-Mai-San to rats // Journal of Eth-nopharmacology. 2008. Vol. 115, N3. Pp. 483–488.
Kim H.J., Oh M.S., Hong J., Jang Y.P. Quantitative analysis of major dibenzocyclooctane lignans in Schisandrae fruc-tus by online TLC-DART-MS // Phytochemical Analysis. 2011. Vol. 22, N3. Pp. 258–262.
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