EVALUATION OF ANTIOXIDANT ACTIVITY OF PLANT AGENTS
UDC 615.32:615.244
Abstract
The aim of the research is to determine the total antioxidant activity of in vitro collection of antihepatotoxic action consisting of roots and rhizomes of Inula helenium L., grass Centaurium erythraea Rafn., flowers Tanacetum vulgare L., fruits Rosa sp., fruits of Crataegus sp. аnd its separate components by the method of colonometric titration and determination of antioxidant activity of dry extract (conditional name "Pentafite") obtained from this collection under conditions of experimental tetrachloride of carbon hepatitis in white rats. A method of chemiluminescent lipid analysis was used to evaluate the free radical oxidation of lipids in liver in test animals. It has been found that collection of antihepatotoxic action and plants included in it have antioxidant activity in vitro, at the same time synergistic effect on manifestation of total antioxidant activity of collection is revealed. Pharmacotherapy of experimental hepatitis in experimental rats by administration of Pentafite at a dose of 300 mg/kg has been found to significantly reduce the chemiluminometric values of lipids, which characterize the intensity of free radical reactions. "Pentafite" has a pronounced inhibitory effect on hyperlipoperoxidation in animal liver in case of toxic damage to it. The established antioxidant activity of collecting antihepatotoxic action and Pentafite extract may be important for their use in the treatment and prevention of hepatobiliary diseases.
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Sherlock S., Dooley J. Diseases of the Liver and Biliary System. 13th edition, London, 2018, 813 p.
Nikolayev S.M. Fitofarmakoterapiya i fitofarmakoprofilaktika zabolevaniy. [Phytopharmacotherapy and phytopharma-coprophylaxis of diseases]. Ulan-Ude, 2012, 286 p. (in Russ.).
Hirschfield G.M., Heathcote E.J., Gershwin M.E. Gastroenterology, 2010, vol. 139, pp. 1481–1496.
Korsun V.F., Nikolayev S.M., Ogrenich N.A. et al. Lekarstvennyye rasteniya i bolezni pecheni. [Medicinal plants and liver diseases]. Moscow, 2014, 320 p. (in Russ.).
Sokolov S.Ya. Fitoterapiya i fitofarmakologiya. Rukovodstvo dlya vrachey. [Phytotherapy and phytopharmacology. A guide for doctors]. Moscow, 2000, 976 p. (in Russ.).
Kurkin V.A., Petrukhina I.K. Fundamental'nyye issledovaniya, 2014, vol. 11, no. 2, pp. 366–371. (in Russ.).
Zhang A., Sun H., Wang X. European Journal of Medicinal Chemistry, 2013, vol. 63, pр. 570–577.
Lubsandorzhiyeva P.-N.B. Razrabotka i standartizatsiya fitosredstv dlya lecheniya i profilaktiki zabolevaniy organov pishchevareniya. [Development and standardization of herbal remedies for the treatment and prevention of diseases of the digestive system]. Ulan-Ude, 2016, 280 p. (in Russ.).
Agrawal A.D. Int. J. Pharmaceutical Sciences and Nanotechnology, 2011, vol. 4, pp. 1394–1398.
Patent 2689379 (RU). 2019. (in Russ.).
Zelenkov V.N., Lapin A.A. Summarnaya antioksidantnaya aktivnost'. Metodika vypolneniya izmereniy na kulono-metricheskom analizatore. MVI-01-00669068-13. [Total antioxidant activity. Procedure for making measurements on a coulometric analyzer. MVI-01-00669068-13]. Vereya, 2013, 19 p. (in Russ.).
Lapin A.A., Romanova N.G., Zelenkov V.N. Primeneniye metoda gal'vanostaticheskoy kulonometrii v opredelenii an-tioksidantnoy aktivnosti razlichnykh vidov biologicheskogo syr'ya i produktov ikh pererabotki. [Application of the meth-od of galvanostatic coulometry in determining the antioxidant activity of various types of biological raw materials and products of their processing]. Moscow, 2011, 197 p. (in Russ.).
Vengerovskiy A.I., Udut V.V., Reykhart D.V. Metodicheskiye rekomendatsii po izucheniyu gepatoprotektivnoy aktivnosti lekarstvennykh sredstv. Rukovodstvo po provedeniyu doklinicheskikh issledovaniy lekarstvennykh sredstv. Ch. I. [Methodical recommendations for the study of hepatoprotective activity of drugs. Guidelines for conducting preclini-cal studies of drugs. Part I]. Moscow, 2012. 832 p. (in Russ.).
Vladimirov Yu.A. Vestnik RAMN, 1988, no. 7, pp. 43–50. (in Russ.).
Folch J., Less M., Sloane-Stanley A.G.H. Journal of Biological Chemistry, 1957, vol. 226, pp. 497–509.
Lapin A.A., Garifullin I.G., Zelenkov V.N. Butlerovskiye soobshcheniya, 2019, vol. 59, no. 7, pp. 91–96. (in Russ.).
Cutler R.G., Rodriquez H. Critical reviews of oxidative stress and aging. Advances in Basic Science, Diagnostics and Intervention, New Jersey, London, Singapore, Hong Kong, 2003, vol. 1, 822 p.
Mates J.M., Perez-Gomez C., De Castro I.N. Clin. Biochem., 1999, vol. 32, pр. 595–603.
Muriel P. Liver Pathophysiology: Therapies and Antioxidants, New York, 2017, 914 p.
Dubininaya Ye.Ye. Produkty metabolizma kisloroda v funktsional'noy aktivnosti kletok (zhizn' i smert', sozidaniye i razrusheniye). Fiziologicheskiye i kliniko-biokhimicheskiye aspekty. [Oxygen metabolism products in the functional ac-tivity of cells (life and death, creation and destruction). Physiological and clinical and biochemical aspects]. St. Peters-burg, 2006, 400 p. (in Russ.).
Bhattacharyya A., Chattopadhyay R., Mitra S., Crave S.E. Physiol. Rev., 2014, vol. 94, pр. 329–354.
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