BIOCHEMICAL COMPOSITION OF VEGETATIVE EXPLANTS AND CALLUS PINUS SIBIRICA DU TOUR

UDC 615.322+58.085

  • Zhanna Aleksandrovna Koh Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS; Krasnoyarsk State Agrarian University Email: jannetta-83@mail.ru
  • Yuliya Aleksandrovna Litovka Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS; Siberian State University of Science and Technology named after academician M.F. Reshetneva Email: litovkajul@rambler.ru
  • Polina Vasil'yevna Makolova Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS Email: polinochka-makolova@rambler.ru
  • Kseniya Aleksandrovna Shabanova Siberian State University of Science and Technology named after academician M.F. Reshetneva Email: shabanova.ksenia@mail.ru
  • Igor' Nikolayevich Pavlov Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS; Siberian State University of Science and Technology named after academician M.F. Reshetneva Email: forester24@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2020048530
Keywords: Pinus sibirica, in vitro cultivation, sterilization, callus, explants, biochemical composition, water-soluble proteins

Abstract

The methods of sterilization of annual shoots Pinus sibirica Du Tour and the conditions for their introduction into in vitro culture were studied. Induction of callusogenesis of aseptically viable explants of P. sibirica proceeds more intensively on the modified Murasige-Skoog medium: hormonal supply of 0.4% kinetin and 0.25% 6-benzylaminopurine; reduced sucrose concentration of 1.5%. The frequency of callus formation was 83%.

Close quantitative indicators of extractive substances were established (36 and 33% of absolutely dry weight for callus and explant, respectively); easily hydrolyzable polysaccharides (18 and 16%) and proteins (11 and 10%).Callus P. sibirica has a higher content of ascorbic acid, flavanoids, tocopherols and ash elements compared to explants and a low amount of hard-hydrolyzable polysaccharides, lipids, tannins, pigments, and essential oils.The electrophoretic spectrum of water-soluble callus proteins is represented by eleven fractions: 63% of the total water-soluble proteins are fractions with a molecular weight of 33 kD and above. Fractions with molecular weights of 50 and 62 kD (20 and 17%, respectively) are represented as much as possible.In the explants of P. sibirica, low molecular weight fractions of proteins with molecular masses of 5 kD and lower (59%) predominate. The amino acid composition of calli and explants of P. sibirica is identical and is represented by fifteen individual amino acids. Callus tissue has a higher content of glutamic acid and two hydrophobic amino acids (proline and isoleucine) compared to the vegetative part of the plant and low tyrosine content.

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Author Biographies

Zhanna Aleksandrovna Koh, Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS; Krasnoyarsk State Agrarian University

кандидат технических наук, научный сотрудник, доцент кафедры технологии, оборудования бродильных и пищевых производств, тел. (391)247-26-66

Yuliya Aleksandrovna Litovka, Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS; Siberian State University of Science and Technology named after academician M.F. Reshetneva

доктор биологических наук, старший научный сотрудник, профессор кафедры химической технологии древесины и биотехнологии, тел. (391) 227-36-54

Polina Vasil'yevna Makolova, Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS

младший научный сотрудник лаборатории лесных культур, микологии и фитопатологии

Kseniya Aleksandrovna Shabanova, Siberian State University of Science and Technology named after academician M.F. Reshetneva

студент

Igor' Nikolayevich Pavlov, Institute of Forest named after V.N. Sukachev SB RAS, FRC KSC SB RAS; Siberian State University of Science and Technology named after academician M.F. Reshetneva

доктор биологических наук, профессор, заведующий лабораторией лесных культур, микологии и фитопатологии, заместитель директора по научной работе, заведующий кафедрой химической технологии древесины и биотехнологии, тел. (391) 227-36-54

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Published
2020-12-21
How to Cite
1. Koh Z. A., Litovka Y. A., Makolova P. V., Shabanova K. A., Pavlov I. N. BIOCHEMICAL COMPOSITION OF VEGETATIVE EXPLANTS AND CALLUS PINUS SIBIRICA DU TOUR // chemistry of plant raw material, 2020. № 4. P. 395-403. URL: http://journal.asu.ru/cw/article/view/8530.
Issue
No 4 (2020)
Section
Technology

Copyright (c) 2020 chemistry of plant raw material

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