ISOLATION, PHYSICO-CHEMICAL CHARACTERISTICS OF POLYSACCHARIDE ISOLATED FROM THE FRUIT BODY OF INONOTUS HISPIDUS

UDC 547.4:577.1:576.8

  • Gulnoza Abduvahobovna Khalilova A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences Email: gulnoza_xalilova@mail.ru
  • Abbaskhan Sabirkhanovich Turaev A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences Email: abbaskhan@mail.ru
  • Bakhtiyor Ikromovich Muhitdinov A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences Email: muhitdinov.bahtiyor@gmail.com
  • Al'bina Vasil'yevna Filatova A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences Email: albfil@mail.ru
  • Saida Bokizhonovna Haytmetova A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences Email: xsb75@mail.ru
  • Nodirali Sokhobatalievich Normakhamatov A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences Email: nodirali@gmail.com
DOI_disc_logo https://doi.org/10.14258/jcprm.2021039028
Keywords: water-soluble polysaccharides, glucose, rhamnose, β-glucan, basidiomycete raw materials, carbohydrates

Abstract

The article is devoted to the study of polysaccharides isolated from the basidiomycete raw material I. hispidus and studying their physical and chemical properties. Water-soluble polysaccharides were isolated from mushroom raw materials by the method of sequential water extraction and the yield was 9.44%. Polysaccharides were separated into neutral fractions by ion exchange chromatography and purified from proteins and peptides. During the separation process, it was determined that the polysaccharide sample consisted of homogeneous polysaccharides, while the carbohydrate content of the purified polysaccharide sample was 99.4%. The carbohydrate composition of polysaccharides was determined, it was found that the polysaccharide consists mainly of glucose residues and contains minor amounts of fructose and rhamnose residues. Molecular weight and molecular weight distribution were determined by size exclusion chromatography. The Mw of the polysaccharide sample obtained was 18.7 kDa, the polydispersity index was 1.3. The results of IR-, 1H- and 13C NMR spectroscopic studies have shown that the polysaccharide, according to its structural characteristics, belongs to the β-glucan type polysaccharide having β-(1,3) and β-(1,6)-glycosidic bounds.

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Author Biographies

Gulnoza Abduvahobovna Khalilova, A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences

базовый докторант

Abbaskhan Sabirkhanovich Turaev, A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences

доктор химических наук, академик, главный научный сотрудник

Bakhtiyor Ikromovich Muhitdinov, A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences

старший научный сотрудник

Al'bina Vasil'yevna Filatova, A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences

доктор технических наук, старший научный сотрудник

Saida Bokizhonovna Haytmetova, A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences

кандидат технических наук, старший научный сотрудник

Nodirali Sokhobatalievich Normakhamatov, A.S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences

доктор химических наук, старший научный сотрудник

References

Simon B., Anke T., Sterner O. Phytochemistry, 1994, vol. 3, pp. 815–816.

Meng X., Liang H., Luo L. Carbohydrate Research, 2016, vol. 424, pp. 30–41. DOI: 10.1016/j.carres.2016.02.008.

Zjawiony J. Journal National Products, 2004, vol. 67, pp. 300–310. DOI: 10.1021 / np030372w.

Chen B. Carbohydrate Polymers, 2010, vol. 81, pp. 420–424. DOI: 10.1016/j.carbpol.2010.02.039.

Masuda Y., Kodama N., Nanba H. Mycoscience, 2006, vol. 47, pp. 360–366. DOI: 10.1007/S10267-006-0315-7.

Wang N. Тianjin Yiyao, 1989, vol. 17, pp. 534–536.

Khalilova G.A., Mukhitdinov B.I., Khaytmetova S.B., Normakhamatov N.S., Turayev A.S. Uzbekskiy khimicheskiy zhurnal, 2020, no. 5, pp. 88–94. (in Russ.).

Azimova L.B., Normakhamatov N.S., Khaytmetova S.B., Mukhitdinov B.I., Amonova D.M., Filatova A.V., Khalilova G.A., Kirgizbayev Kh.Kh., Turayev A.S. Khimiya rastitel'nogo syr'ya, 2019, no. 2, pp. 35–41. DOI: 10.14258/jcprm.2019024491. (in Russ.).

Khalilova G.A., Mukhitdinov B.I., Khaytmetova S.B., Azimova L.B., Normakhamatov N.S., Turayev A.S. Farmatsevticheskiy zhurnal, 2019, no. 4, pp. 72–83. (in Russ.).

Dubois M., Gilles K.A., Hamilton J., Robers P.A., Smith F. Analyt. Chem., 1956, vol. 28, pp. 350–356.

Staub A.M. Methods in Carbohydrate Chemistry, 1965, pp. 5–6.

Ye T. et al. Microbiology, 2008, vol. 154, pp. 2814–2826. DOI: 10.1099/mic.0.2008/020149-0.

Jianguo W., Yahong Y., Tianli Y. Carbohydrate Polymers, 2014, vol. 10, pp. 247–254. DOI: 10.1016/j.carbpol.2013.10.087.

Pan F., Su T.J., Liu Y., Hou K., Chen C., Wu W. International Journal of Biological Macromolecules, 2018, vol. 112, pp. 1073–1083. DOI: 10.1016/j.ijbiomac.2018.02.070.

Samanta S., Maity K., Nandi A.K., Sen I.K., Devi K.S.P., Mukherjee S. et al. Carbohydrate Research, 2013, vol. 367, pp. 33–40. DOI: 10.1016/j.carres.-2012.12.002.

Wang Y., Liu Y., Yu H., Zhou S., Zhang Z., Wu D. et al. Carbohydrate Polymers, 2017, vol. 167, pp. 337–344. DOI: 10.1016/j.carbpol.2017.03.016.

Vidal S., Doco T., Williams P., Pellerin P., York W., O’Neill M., Glushka J., Darvill G., Albersheim P. Carbohydrate Research, 2000, vol. 326, pp. 277–294. DOI: 10.1016/s0008-6215(00)00036-7.

Muhitdinov B., Heinze T., Normakhamatov N., Turaev A. Carbohydrate Polymers, 2017, vol. 173, pp. 631–637. DOI: DOI: 10.1016/j.carbpol.2017.06.033.

Muhitdinov B., Heinze T., Turaev A., Koschella A., Normakhamatov N. European Polymer Journal, 2019, vol. 119, pp. 181–188. DOI: 10.1016/j.eurpolymj.2019.07.030.

Dong Q., Wang Y., Shi L., Yao J., Li J., Ma F., Ding K. Carbohydrate Research, 2012, vol. 353, pp. 100–105. DOI: 10.1016/j.carres.2012.02.029.

Published
2021-09-27
How to Cite
1. Khalilova G. A., Turaev A. S., Muhitdinov B. I., Filatova A. V., Haytmetova S. B., Normakhamatov N. S. ISOLATION, PHYSICO-CHEMICAL CHARACTERISTICS OF POLYSACCHARIDE ISOLATED FROM THE FRUIT BODY OF INONOTUS HISPIDUS // chemistry of plant raw material, 2021. № 3. P. 99-106. URL: http://journal.asu.ru/cw/article/view/9028.
Issue
No 3 (2021)
Section
Biopolymers of plants

Copyright (c) 2021 chemistry of plant raw material

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