QUANTITATIVE CONTENT OF THE MAIN ACTIVE SUBSTANCES IN THE ROOTS OF THE NATURAL AND IN-TRODUCED PLANT SAPOSHNIKOVIA DIVARICATE (TURCZ.) SCHISCHK.
UDC 615.074
Abstract
A HPLC-UV method was developed for the quantitative analysis of chromones in the roots of Saposhnikovia divaricata (Turcz.) Schischk. The optimal parameters of chromones extraction (two-fold ultrasonic extraction with a duration of 40 min with 50% ethyl alcohol at a raw material-extractant ratio of 1:10) were selected. The validation analysis showed that the proposed method is characterized by satisfactory metrological parameters. The method was used for the analysis of raw materials of wild, introduced and commercial samples of S. divaricata. In this work, for the first time in the roots of S. divaricata from natural populations of Buryatia, Trans-Baikal Territory, Mongolia and in the introduced samples, the HPLC-UV method was used to determine the quantitative content of the main active substances: prim-O-glucosylcimifugine (0.13–5.22 mg/g), cimifugine (0.01– 1.82 mg/g) and 4’-O-β-D-glucosyl-5-O-methylvisamminol (0.98–3.25 mg/g). The total content of chromones varies depending on the place of collection and the phase of vegetation. The content of chromones in two samples of roots from introduced S. divaricatae, collected in the first year of introduction in different climatic conditions, was established.
Downloads
Metrics
References
Pimenov M.G., Ostroumova T.A. Zontichnyye (Umbelliferae) Rossii. [Umbelliferae of Russia]. Moscow, 2012, 477 p. (in Russ.).
Rastitel'nyye resursy Rossii. Dikorastushchiye tsvetkovyye rasteniya, ikh komponentnyy sostav i biologicheskaya ak-tivnost'. T. 3. Semeystva Fabaceae-Apiaceae [Plant resources of Russia. Wild flowering plants, their component com-position and biological activity. Vol. 3. Family Fabaceae-Apiaceae], ed. A.L. Budantsev. St.-Petersburg, Moscow, 2010, 256 p. (in Russ.).
Yang M., Wang C., Wang W., Xu J., Wang J. Zhang C., Li M. Chin. J. Integr. Med., 2020, vol. 26, pp. 873–880. DOI: 10.1007/s11655-020-3091-x.
Urbagarova B.M., Shults E.E., Taraskin V.V., Radnaeva L.D., Petrova T.N., Rybalova T.V., Frolova T.S., Pokrovskii A.G., Ganbaatar J. J. of Ethnopharmacology, 2020, vol. 261, 112517. DOI: 10.1016/j.jep.2019.112517.
Kreiner J., Pang E., Lenon G.B., Yang A.W.H. Chin. J. of Nat. Med., 2017, vol. 15, no. 4, pp. 255–264. DOI: 10.1016/S1875-5364(17)30042-0.
Urbagarova B.M., Taraskin V.V. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2019, vol. 22, no. 10, pp. 25−29. (in Russ.).
Urbagarova B.M., Taraskin V.V., Shults E.E., Radnaeva L.D., Anenkhonov O.A., Ganbaatar Zh., Boldanova N.B. Chemistry of Natural Compounds, 2017, vol. 53, no. 1, pp. 138–140.
Yang J.-M., Jiang H., Dai H.-L., Wang Z.-W., Jia G.-Zh., Meng X.-C. Pharmacognosy Magazine, 2017, vol. 13, no. 49, pp. 168–174. DOI: 10.4103/0973-1296.197637.
Urbagarova B.M., Taraskin V.V., Radnayeva L.D. Vestnik Buryatskogo gosudarstvennogo universiteta. Biologiya. Geografiya, 2016, vol. 2, no. 3, pp. 100–104. (in Russ.).
Korsun O.V. Stepnoy byulleten', 2018–2019, no. 51–52, pp. 49–51. (in Russ.).
Gosudarstvennaya farmakopeya Rossiyskoy Federatsii: v 4 tomakh, XIV izd. [State Pharmacopoeia of the Russian Federation: in 4 volumes, XIV ed.]. Moscow, 2018. URL: http://femb.ru/femb/pharmacopea.php. (in Russ.).
Committee for the Pharmacopoeia of PR China. Pharmacopoeia of PR China (Part I). Beijing: People's Medical Publishing House, 2015, 150 p.
Li W., Wang Z., Chen L., Zhang J., Han L., Hou J., Zheng Y. J. Sep. Sci., 2010, vol. 33, pp. 2881– 2887. DOI: 10.1002/jssc.201000336.
Seo U.M., Zhao B.T., Kim Y.H., Kang J.S., Son J.K., Woo M.H. Arch. Pharm. Res., 2016, vol. 39, pp. 695–704. DOI: 10.1007/s12272-016-0740-x.
Yu X., Niu Y., Zheng J., Liu H., Jiang G., Chen J., Hong M. Phytomedicine, 2015, vol. 22, no. 13, pp. 1150–1158. DOI: 10.1016/j.phymed.2015.09.002.
Kim M.K., Yang D-H., Jung M., Jung E.H., Eom H.Y., Suh J.H., Min J., Kim U., Min H., Kim J., Han S.B. J. of Chromatography A, 2011, vol. 1218, pp. 6319–6330. DOI: 10.1016/j.chroma.2011.06.103.
Scherubl R., Manns D., Heilmann J., Franz G. Chromatographia, 2013, vol. 76, pp. 1537–1543. DOI: 10.1007/s10337-013-2518-3
Urbagarova B.M. Farmakognosticheskoye issledovaniye sapozhnikovii rastopyrennoy (Saposhnikovia divaricata (Turcz.) Schischkin) korney i razrabotka na ikh osnove ekstrakta sukhogo: dis. ... kand. farm. nauk. [Pharmacognostic study of shoemaker's spreading (Saposhnikovia divaricata (Turcz.) Schischkin) roots and development of dry extract on their basis: dis. ... Cand. farm. sciences]. Ulan-Ude, 2019, 162 p. (in Russ.).
Jian H., Yang J.M., Jia G.Z., Dai H.L., Cao L., Meng X.C. Russian J. of Plant Physiology, 2018, vol. 65, no. 5, pp. 680–687. DOI: 10.1134/S1021443718050084.
Zeng L.J., Sun Q.S., Jia L.Y. J. Shenyang Pharm. Univ., 2009, vol. 26, no. 2, pp. 127–130.
Copyright (c) 2021 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
