CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF THE DRY EXTRACT "ROSMATIN" FROM THE HERB OF DRACOCEPHALUM MOLDAVICA L.

UDC 615.322; 543.429.23; 543.544.5.068.7; 615.07;615.243

  • Olga Petrovna Sheychenko All-Russian Scientific Research Institute of Medicinal and Aromatic Plants Email: sheychenko@vilarnii.ru
  • Vladimir Ivanovich Sheychenko All-Russian Scientific Research Institute of Medicinal and Aromatic Plants Email: sheychenko@vilarnii.ru
  • Sergey Vladimirovich Goryainov Peoples' Friendship University of Russia Email: sheychenko@vilarnii.ru
  • Ekaterina Vladimirovna Zvezdina All-Russian Scientific Research Institute of Medicinal and Aromatic Plants Email: catterina@inbox.ru
  • Elena Nikolaevna Kurmanova All-Russian Scientific Research Institute of Medicinal and Aromatic Plants Email: sheychenko@vilarnii.ru
  • Ekaterina Vladimirovna Ferubko All-Russian Scientific Research Institute of Medicinal and Aromatic Plants Email: sheychenko@vilarnii.ru
  • Elena Viktorovna Uyutova All-Russian Scientific Research Institute of Medicinal and Aromatic Plants Email: sheychenko@vilarnii.ru
  • Ol'ga Georgiyevna Potanina Peoples' Friendship University of Russia Email: sheychenko@vilarnii.ru
  • Fadi Khazhzhar Peoples' Friendship University of Russia Email: sheychenko@vilarnii.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2021039161
Keywords: Dracocephalum moldavica L., Rozmatin dry extract, 1H- and 13C-NMR, HPLC-DAD-MS, polyphenols, flavonoids (flavones), rosmarinic acid, anti-inflammatory and gastroprotective activity

Abstract

Moldavian Dragonhead (Dracocephalum moldavica L.) is an annual herb. In FGBNU VILAR a new variety of Moldavian Dragonhead «Nezhnost» has been developed. From the herb of the Moldavian Dragonhead, a technology has been developed for obtaining a dry purified extract, which has received the code name «Rozmatin».

By NMR (1H and 13C) and HPLC-DAD-MS using standard samples and literature data, the qualitative and quantitative composition of the dry extract "Rozmatin" from the herb of the Dracocephalum moldavica was established. Rosmarinic, caffeic and ferulic acids, rosmarinic acid 3-O-glucoside, luteolin-7-O-β-D-glucuronide, acacetin-7-O-β-D-glucopyranoside (tilianin), apigenin-7-O-β-D-glucoside (Cosmosiin), apigenin-7-O-β-D-glucuronide, chrysoeryol-7-O-β-D-glucuronide, apigenin-7-O-β-D-(6ʺ-O-malonyl) glucoside, acacetin-7-O-β-D-glucuronide, acacetin-7-O-β-D-(6ʺ-malonyl) glucoside, schizotenuin F were identified. It was found that the content of rosmarinic acid in "Rosmatin" is 56.6%, the amount of flavonoids - about 27%. The study of anti-inflammatory and gastroprotective activity of "Rozmatin" in doses of 10 mg/kg, 100 mg/kg was carried out. A dose-dependent anti-inflammatory effect with suppression of the development of the exudative phase of inflammation, as well as a dose-dependent gastroprotective effect of "Rozmatin"", significantly exceeding the similar effect of the referent drug omeprazole, was revealed. Earlier it was found that "Rozmatin" has a tonic effect on the cardiovascular and nervous systems.

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Author Biographies

Olga Petrovna Sheychenko, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

кандидат химических наук, ведущий научный сотрудник отдела химии природных соединений

Vladimir Ivanovich Sheychenko, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

кандидат физико-математических наук, ведущий научный сотрудник отдела химии природных соединений

Sergey Vladimirovich Goryainov, Peoples' Friendship University of Russia

заведующий лабораторией масс-спектрометрии высокого разрешения и спектроскопии ЯМР

Ekaterina Vladimirovna Zvezdina, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

старший научный сотрудник отдела химии природных соединений

Elena Nikolaevna Kurmanova, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

научный сотрудник отдела экспериментальной фармакологии

Ekaterina Vladimirovna Ferubko, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

кандидат медицинских наук, заведующая отделом экспериментальной фармакологии

Elena Viktorovna Uyutova, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

научный сотрудник отдела химии природных соединений

Ol'ga Georgiyevna Potanina, Peoples' Friendship University of Russia

доктор фармацевтических наук, директор центра научных исследований и разработок

Fadi Khazhzhar, Peoples' Friendship University of Russia

аспирант

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Published
2021-09-27
How to Cite
1. Sheychenko O. P., Sheychenko V. I., Goryainov S. V., Zvezdina E. V., Kurmanova E. N., Ferubko E. V., Uyutova E. V., Potanina O. G., Khazhzhar F. CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF THE DRY EXTRACT "ROSMATIN" FROM THE HERB OF DRACOCEPHALUM MOLDAVICA L. // chemistry of plant raw material, 2021. № 3. P. 253-264. URL: http://journal.asu.ru/cw/article/view/9161.
Issue
No 3 (2021)
Section
Low-molecular weight compounds

Copyright (c) 2021 chemistry of plant raw material

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