DEVELOPMENT OF METHODS OF STANDARDIZATION OF FLOWERS OF SPREADING MARIGOLD (TAGETES PATULA L.)
UDC 615.322: 547.972 +543.544
Abstract
The spreading marigold (Tagetes patula L.), the family Aster (Asteraceae), or Composite (Compositae), is a prospective source of flavonoids. The aim of the study is to develop methods of standardization of marigold flowers rejected, consisting in determining the identity of this raw material and the quantitative determination of biologically active compounds. As a result of a comparative chromatographic study, the presence of flavonoids was detected upon detection at a wavelength of 254 and 366 nm and before and after reaction with an alcohol solution of aluminum chloride. The presence of patuletin and patulitrin was confirmed in extracts of different varietal forms of Tagetes patula L.. Using column chromatography on silica gel L 40/100, two flavonoid substances were isolated patuletin (3,5,7,3′,4′-pentahydroxy-6-methoxyflavone) and patulitrin (7-O-β-D-glucopyranoside of 3,5,7,3',4′-pentahydroxy-6-methoxyflavone), which have been identified by UV-, 1H-NMR -, 13C-NMR-spectroscopy and mass spectrometry, as well as acid hydrolysis. In the solvent system chloroform- ethanol-water (26 : 16 : 3) Rf values of patuletin and patulitrin are about 0.7 and 0.4, respectively. It was found that patulitrin is the main flavonoid in the flowers of spreading marigold and determines the character of the absorption curve of the electronic spectra of water-alcohol extraction from this raw material. During the quantitative analysis of the total flavonoids in the flowers of spreading marigold, it is necessary to recalculate the content of active substances to the dominant and diagnostically significant flavonoid patulitrin. It was determined that the content of the total flavonoids in the studied samples of flowers of different varieties of the spreading marigold varies from 4.36±0.02% to 11.71±0.05% (calculated on patulitrin).
Downloads
Metrics
References
Yershova I.B., Osipova T.F. Aktual'naya Infektologiya, 2016, no. 4 (13), pp. 73–81. DOI: 10.22141/2312-413x.4.13.2016.91460. (in Russ.).
Kurkina A.V., Galyamova V.R., Kurkin V.A., Avdeyeva Ye.V. Farmatsiya i farmakologiya, 2016, vol. 4, no. 2 (15), pp. 26–40. DOI: 10.19163/2307-9266-2016-4-2(15)-26-40. (in Russ.).
Kurkin V.A., Avdeyeva Ye.V., Kurkina A.V., Pravdivtseva O.Ye., Braslavskiy V.B. Meditsinskiy vestnik Bashkorto-stana, 2016, vol. 11, no. 5 (65), pp. 149–152. (in Russ.).
Zaytseva Ye.N., Dubishchev A.V., Kurkin V.A. Nauka i innovatsii v meditsine, 2016, no. 4, pp. 47–50. (in Russ.).
Zimenkina N.I., Kurkin V.A. Aspirantskiy vestnik Povolzh'ya, 2020, no. 1–2, pp. 131–136. DOI: 10.17816/2072-2354.2020.20.1.131-136. (in Russ.).
Kurkina A.V. Flavonoidy farmakopeynykh rasteniy: monografiya. [Flavonoids of pharmacopoeial plants: monograph]. Samara, 2012, 290 p. (in Russ.).
Gosudarstvennaya farmakopeya Rossiyskoy Federatsii. XIV izd. [State Pharmacopoeia of the Russian Federation. XIV ed.]. Moscow, 2018, vol. I–IV. URL: http://http://femb.ru/femb/pharmacopea.php. (in Russ.).
Lomkina Ye.M., Chervonnaya N.M., Kurkin D.V., Volotova Ye.V., Bakulin D.A., Oganesyan E.T., Andreyeva O.A., Tyurenkov I.N. Farmatsiya, 2016, vol. 65, no. 3, pp. 37–39. (in Russ.).
Papayani O.I., Dukhanina I.V., Sergeyeva O.Ye. Izvestiya Samarskogo nauchnogo tsentra RAN, 2012, vol. 14, no. 5(3), pp. 742–744. (in Russ.).
Nikolayevskiy V.V. Aromaterapiya: spravochnik. [Aromatherapy: A Handbook]. Moscow, 2000, 366 p. (in Russ.).
Kholikova O., Azonov D.A., Ganiyev Kh.A. Colloquium-journal, 2019, no. 11-2 (35), pp. 49–52. DOI: 10.24411/2520-6990-2019-10309. (in Russ.).
Malyugina Ye.A., Mazulin A.V., Smoylovskaya G.P. Nauchnyye trudy SWorld, 2015, vol. 18, no. 2 (39), pp. 48–52. (in Russ.).
Podgornaya Zh.V. Issledovaniye tsvetkov barkhattsev rasprostertykh (Tagetes patula L.) s tsel'yu polucheniya bio-logicheski aktivnykh soyedineniy: diss. … kand. farm. nauk. [Study of flowers of prostrate marigolds (Tagetes patula L.) in order to obtain biologically active compounds: diss. … cand. farm. Sciences]. Pyatigorsk, 2008. (in Russ.).
Samosudova I.Ye., Boyko N.N., Tsvetkov Z.Ye. Sovremennyye tendentsii razvitiya tekhnologiy zdorov'yesberezheniya. [Modern trends in the development of health saving technologies]. Moscow, 2019, pp. 315–319. (in Russ.).
Chervonnaya N.M. Traditsionnaya i innovatsionnaya nauka: istoriya, sovremennoye sostoyaniye, perspektivy, 2017, pp. 134–138. (in Russ.).
Chervonnaya N.M., Andreyeva O.A., Adzhiakhmetova S.L., Oganesyan E.T. Khimiya rastitel'nogo syr'ya, 2018, no. 3, pp. 91–98. DOI: 10.14258/jcprm.2018033714. (in Russ.).
Chervonnaya N.M., Andreyeva O.A., Kharchenko I.I. Nauchnyye vedomosti Belgorodskogo gosudarstvennogo uni-versiteta. Seriya: Meditsina. Farmatsiya, 2016, no. 26 (247), pp. 147–151. (in Russ.).
Chervonnaya N.M., Oganesyan E.T., Andreyeva O.A., Senchenko S.P., Borovskiy B.V. Zhurnal nauchnykh statey Zdorov'ye i obrazovaniye v XXI veke, 2017, vol. 19, no. 6, pp. 132–137. (in Russ.).
Chervonnaya N.M., Kharchenko I.I., Adzhiakhmetova S.L., Mykots L.P., Andreyeva O.A., Oganesyan E.T. Far-matsiya i farmakologiya, 2017, vol. 5, no. 3, pp. 267–282. DOI: 10.19163/2307-9266-2017-5-3-267-282. (in Russ.).
Benea A., Ciobanu C., Cojocaru-Toma M., Ciobanu N. The Moldovan Medical Journal, 2020, vol. 63, no. 4, pp. 23–26. DOI: 10.5281/zenodo.4016806.
Deepshikha K., Yashodhara V. Annual Research & Review in Biology, 2017, vol. 13, no. 6, pp. 1–8. DOI: 10.9734/ARRB/2017/34349.
Gongadze M., Machavariani M., Enukidze M., Gogia N., Iobadze M., Chkhikvishvili I. Georgian Med. News, 2019, vol. 297, pp. 154–157.
Meurer M.C., Mees M., Mariano L.N., Boeing T., Somensi L.B., Mariott M.M., Da Silva R.D., Dos Santos A.C.D., Longo B., França T.C.S., Klein-Junior L., de Souza P., de Andrade S.F., da Silva L.M. Nutrition research, 2019, vol. 66, pp. 95–106. DOI: 10.1016/j.nutres.2019.03.005.
Nawale S., Padma Priya K., Pranusha P., Ganga Raju M. Data in Brief, 2018, vol. 21, pp. 587–597. DOI: 10.1016/j.dib.2018.10.022.
![](http://journal.asu.ru/public/journals/1/article_9243_cover_ru_RU.jpg)
Copyright (c) 2022 chemistry of plant raw material
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.