DEVELOPMENT OF METHODS OF STANDARDIZATION OF FLOWERS OF SPREADING MARIGOLD (TAGETES PATULA L.)

UDC 615.322: 547.972 +543.544

DOI_disc_logo https://doi.org/10.14258/jcprm.2022049243
Keywords: Spreading marigold, Tagetes patula L., flowers, flavonoids, patulitrin, spectrophotometry, chromatographic analysis, standardization

Abstract

The spreading marigold (Tagetes patula L.), the family Aster (Asteraceae), or Composite (Compositae), is a prospective source of flavonoids. The aim of the study is to develop methods of standardization of marigold flowers rejected, consisting in determining the identity of this raw material and the quantitative determination of biologically active compounds. As a result of a comparative chromatographic study, the presence of flavonoids was detected upon detection at a wavelength of 254 and 366 nm and before and after reaction with an alcohol solution of aluminum chloride. The presence of patuletin and patulitrin was confirmed in extracts of different varietal forms of Tagetes patula L.. Using column chromatography on silica gel L 40/100, two flavonoid substances were isolated patuletin (3,5,7,3′,4′-pentahydroxy-6-methoxyflavone) and patulitrin (7-O-β-D-glucopyranoside of 3,5,7,3',4′-pentahydroxy-6-methoxyflavone), which have been identified by UV-, 1H-NMR -, 13C-NMR-spectroscopy and mass spectrometry, as well as acid hydrolysis. In the solvent system chloroform- ethanol-water (26 : 16 : 3) Rf values of patuletin and patulitrin are about 0.7 and 0.4, respectively. It was found that patulitrin is the main flavonoid in the flowers of spreading marigold and determines the character of the absorption curve of the electronic spectra of water-alcohol extraction from this raw material. During the quantitative analysis of the total flavonoids in the flowers of spreading marigold, it is necessary to recalculate the content of active substances to the dominant and diagnostically significant flavonoid patulitrin. It was determined that the content of the total flavonoids in the studied samples of flowers of different varieties of the spreading marigold varies from 4.36±0.02% to 11.71±0.05% (calculated on patulitrin).

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Author Biographies

Vladimir Aleksandrovich Kurkin, Samara State Medical Universit

доктор фармацевтических наук, профессор, заведующий кафедрой фармакогнозии с ботаникой и основами фитотерапии

Anna Evgen'evna Saveleva, Samara State Medical Universit

аспирант

Anna Vladimirovna Kurkina, Samara State Medical Universit

доктор фармацевтических наук, доцент, доцент кафедры фармакогнозии с ботаникой и основами фитотерапии

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Published
2022-12-15
How to Cite
1. Kurkin V. A., Saveleva A. E., Kurkina A. V. DEVELOPMENT OF METHODS OF STANDARDIZATION OF FLOWERS OF SPREADING MARIGOLD (TAGETES PATULA L.) // chemistry of plant raw material, 2022. № 4. P. 219-229. URL: http://journal.asu.ru/cw/article/view/9243.
Issue
No 4 (2022)
Section
Low-molecular weight compounds

Copyright (c) 2022 chemistry of plant raw material

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