INVESTIGATION OF SUPERCRITICAL CO2-EXTRACTS OF WILD LEDUM PALUSTRE L. (RHODODENDRON TO-MENTOSUM HARMAJA) AND IDENTIFICATION OF ITS METABOLITES BY TANDEM MASS SPECTROMETRY

UDC 615.322

  • Maya Petrovna Razgonova Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov; Far Eastern Federal University Email: m.razgonova@vir.nw.ru
  • Aleksandr Mikhaylovich Zakharenko Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov; Far Eastern Federal University; Siberian Federal Scientific Center for Agrobiotechnologies RAS Email: zakharenko.am@dvfu.ru
  • Kirill Sergeyevich Golokhvast Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov; Far Eastern Federal University; Siberian Federal Scientific Center for Agrobiotechnologies RAS; Pacific Institute of Geography FEB RAS Email: golokhvast@sfsca.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2022019506
Keywords: Ledum palustre, Rhododendron tomentosum, tandem mass spectrometry, polyphenols, rhododendron

Abstract

The purpose of this research is to investigate and identify polyphenolic complexes and other biologically active compounds by tandem mass spectrometry, presented in the leaves and stems of Ledum palustre L. Carbon dioxide, compressed to a supercritical state, was used for the most environmentally friendly extraction of polyphenolic complexes and other biologically active compounds of Ledum palustre L. The most effective extraction characteristics (pressure 350 bar; temperature 60 °С; extraction time 1-hour, co-solvent MeOH 3.5%) supercritical CO2-extraction of L. palustre were obtained empirically. To identify target analytes in supercritical extracts, High Performance Liquid Chromatography (HPLC) was used in combination with a BRUKER DALTONIKS ion trap. The results showed the presence of 61 biologically active compounds corresponding to the rhododendron species, of which 32 were identified for the first time in L. palustre. These are flavanols dihydrokaempferol, quercetin arabinoside, myricetin galactoside; flavones: diosmetin, nevadensin, cirsimaritin; flavanone naringenin; anthocyanins delphinidin, petunidin, cyanidin pentoside, delphinidin pentoside, peonidin 3-(6-O-acetyl) glucoside, peonidin-3-O-malonylglucoside, cyanidin-3-rutinoside, peonidin 3-O-glucoside; ellagic acid; lignan medioresinol; a type A procyanidin dimer; sterols fucosterol and avenasterol, etc.

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Author Biographies

Maya Petrovna Razgonova, Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov; Far Eastern Federal University

кандидат технических наук, ВРИО директора Дальневосточной опытной станции

Aleksandr Mikhaylovich Zakharenko, Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov; Far Eastern Federal University; Siberian Federal Scientific Center for Agrobiotechnologies RAS

кандидат химических наук, ведущий научный сотрудник, заместитель директора по научно-технической работе

Kirill Sergeyevich Golokhvast, Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov; Far Eastern Federal University; Siberian Federal Scientific Center for Agrobiotechnologies RAS; Pacific Institute of Geography FEB RAS

доктор биологических наук, член-корреспондент РАО, профессор, директор

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Published
2022-03-10
How to Cite
1. Razgonova M. P., Zakharenko A. M., Golokhvast K. S. INVESTIGATION OF SUPERCRITICAL CO2-EXTRACTS OF WILD LEDUM PALUSTRE L. (RHODODENDRON TO-MENTOSUM HARMAJA) AND IDENTIFICATION OF ITS METABOLITES BY TANDEM MASS SPECTROMETRY // chemistry of plant raw material, 2022. № 1. P. 179-191. URL: http://journal.asu.ru/cw/article/view/9506.
Issue
No 1 (2022)
Section
Low-molecular weight compounds

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