SUPERCRITICAL FLUID EXTRACTION OF CAROTENOIDS AND CHLOROPHYLL FROM LEDUM PALUSTRE

  • Александра (Aleksandra) Сергеевна (Sergeevna) Попова (Popova) Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002 Email: alex772977@gmail.com
  • Артем (Artem) Дмитриевич (Dmitrievich) Ивахнов (Ivakhnov) Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002 Email: ivahnov-tema@yandex.ru
  • Татьяна (Tat'iana) Эдуардовна (Eduardovna) Скребец (Skrebets) Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002 Email: tskrebets@mail.ru
  • Константин (Konstantin) Григорьевич (Grigor'evich) Боголицын (Bogolitsyn) Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002 Email: k.bogolitsin@narfu.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2018012994
Keywords: biologically active agents, Labrador tea marsh, supercritical fluid extraction, carbon dioxide, chlorophyll, carotinoids

Abstract

The purpose of this work is to investigate the influence of supercritical fluid extraction parameters and the quality of the feedstock on the yields of pigments from the Led Flyweed. As a method of extracting the target components, supercritical fluid extraction was used. To quantify the target components, spectrophotometric and chromatographic methods were used, antioxidant activity was determined on the anti-oxidant activity analyzer «Blizar». Comparison of the conditions for the isolation of chlorophylls and carotenoids of Ledum palustre (Ledum palustre) when using supercritical carbon dioxide and ethyl alcohol as extractants is compared. The parameters of supercritical fluid extraction for obtaining extracts enriched with one or both pigments are determined. It is established that the variation of the pressure and temperature of the fluid, the duration of processing and the moisture content of the raw material allows one to extract extracts enriched with one or both extractable pigments. The optimum amount of ethanol used as cosolvent is 5 volume% and is necessary and sufficient for efficient extraction of pigments by supercritical CO2. The data obtained are significant for the pharmaceutical, food and perfume and cosmetic industries, where natural dyes and antioxidants are required. The problem of developing their production and isolation is solved. Technologies are being developed for obtaining pigments from plants, which were previously widely used for the manufacture of medicines.

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Author Biographies

Александра (Aleksandra) Сергеевна (Sergeevna) Попова (Popova), Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002
engineer
Артем (Artem) Дмитриевич (Dmitrievich) Ивахнов (Ivakhnov), Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002
Teacher of the Department of Theoretical and Applied Chemistry
Татьяна (Tat'iana) Эдуардовна (Eduardovna) Скребец (Skrebets), Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002
Associate Professor of the Department of Theoretical and Applied Chemistry
Константин (Konstantin) Григорьевич (Grigor'evich) Боголицын (Bogolitsyn), Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb., 17, Arkhangelsk, 163002
Head of the Department of Theoretical and Applied Chemistry

References

Zilfikarov I.N., Chelombit'ko A.V., Aliev A.M. Obrabotka lekarstvennogo rastitel'nogo syr'ia szhizhen-nymi gazami i sverkhkriticheskimi fliuidami. [Treatment of medicinal plant raw materials with liquefied gases and supercritical fluids]. Piatigorsk, 2007, 244 p. (in Russ.).

Arai Y., Sako T., Takebayashi Y. Supercritical fluids: molecular interactions, physical properties and new applica-tions, Berlin: Springer, 2002, 345 p.

Reverchon E., De Marco I. J. of Supercritical Fluids, 2006, pp. 146–166.

Popovici C., Gitin L., Alexe P. Journal of Food and Packaging Science, Technique and Technologies, 2013, pp. 104–108.

Macias-Sanchez M.D. J. of Supercritical Fluids, 2007, vol. 39, pp. 323–329.

Macias-Sanchez M.D. J. Sep. Sci., 2008, vol. 31, pp. 1352–1362.

Catarina Guedes А. J. of Food Engineering, 2013, pp. 478–482.

Macias-Sanchez M.D. J. of Food Engineering, 2013, pp. 245–251.

Duarte С., Moldаo-Martins М., Gouveia А., Beirаo da Costa S., Leitao A.E., Bernardo-Gil M.G. The Journal of Su-percritical Fluids, 2004, vol. 30, pp. 155–161.

Ivakhnov A.D., Skrebets T.E., Bogdanov M.V., Bogolitsyn K.G., Tremzina E.D. Khimiia rastitel'nogo syr'ia, 2013, no. 4, pp. 65–69. (in Russ.).

Amosova A., Ivahnov A., Skrebets T., Ulyanovskiy N., Bogolitsyn K. Russian Journal of Physical Chemistry B, 2014, vol. 8, no. 7, pp. 63–66.

Ul'ianovskii N.V., Kosiakov D.S., Bogolitsyn K.G., Ivakhnov A.D., Amosova A.S. Khimiia rastitel'nogo syr'ia, 2012, no. 4, pp. 147–152. (in Russ.).

Antonov V.I., Iagodin V.I. Khimiia rastitel'nogo syr'ia, 2006, no. 2, pp. 47–49. (in Russ.).

Ribeiro B.D. Food Bioprocess Technol., 2011, vol. 4, pp. 693–701.

Patent 267816 (USSR). 1970. (in Russ.).

Deila B. Rodriguez Amaya. A guide to carotenoid analysis in foods. USA, Washington D.C.: ILSI Press, 2001, 65 p.

Blois M.S. Nature, 1958, vol. 181, pp. 1199–1200.

Elizabeth K. J. Pharmaceu., 1990, vol. 58, pp. 237–240.

Oyaizu M. Jpn. J. Nutr., 1986, pp. 307–315.

Khasanov V.V., Ryzhova G.L., Mal'tseva E.V. Khimiia rastitel'nogo syr'ia, 2004, no. 3, pp. 77–85. (in Russ.).

Marelli de Souza L., Ferreira K.S., Paes Chaves J.B., Teixeira S.L. Scientia Agricola., 2008, vol. 65, no. 3, pp. 246–250.

Bushway R.J., Wilson A.M. Can. Inst. Food. Sci. Technol. J., 1982, vol. 15, pp. 165–169.

Published
2017-10-09
How to Cite
1. Попова (Popova)А. (Aleksandra) С. (Sergeevna), Ивахнов (Ivakhnov)А. (Artem) Д. (Dmitrievich), Скребец (Skrebets)Т. (Tat’iana) Э. (Eduardovna), Боголицын (Bogolitsyn)К. (Konstantin) Г. (Grigor’evich) SUPERCRITICAL FLUID EXTRACTION OF CAROTENOIDS AND CHLOROPHYLL FROM LEDUM PALUSTRE // chemistry of plant raw material, 2017. № 1. P. 61-66. URL: http://journal.asu.ru/cw/article/view/2994.
Issue
No 1 (2018)
Section
Low-molecular weight compounds

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