Abstract
Copper is an essential micronutrient pivotal for plant physiological processes, and its nanoformulations have garnered significant attention for their potential to enhance crop productivity. This study evaluated the comparative efficacy of different forms of copper, copper sulfate (CuSO₄), copper-EDTA chelate and copper oxide nanoparticles (CuO NPs), on the germination, vegetative growth, and yield of two pinto bean cultivars (Phaseolus vulgaris L.) (KS1191 and KS1193) using a seed priming approach. The experiment was arranged in a randomized complete block design (RCBD) with nine treatments and three replications. Treatments included CuSO₄ (0.3%), CuO NPs (0.05%, 0.1%, 0.15%), copper-EDTA (0.1%, 0.3%, 0.5%), a combination of 0.1% CuO NPs with 0.3% copper-EDTA, and an untreated control. We evaluated the impact on seed germination percentage, seedling vigor index, vegetative growth (plant height), yield components (pod number and ten seed weight), and seed copper accumulation. Analysis of variance (ANOVA) revealed significant effects of treatment on all measured parameters. In particular, seed priming with CuO NPs, particularly at the 0.1% concentration, elicited significant improvements in vegetative growth, yield, and seed copper content compared to the control and other copper forms. The findings indicate that seed priming with 0.1% CuO NPs represents an optimal strategy for enhancing growth and yield in pinto bean cultivation, highlighting the advantages of nanoformulations in micronutrient delivery.
References
Abdual-baki AA, Anderson JD (1973) Relationship between decarboxylation of glutamic acid and vigour in soybean seed. Crop Science 13: 222-226. https://doi.org/10.2135/cropsci1973.0011183X001300020023x
Cui H, Sun C, Liu Q, Jiang J, Gu W (2006) Applications of Nanotechnology in Agrochemical Formulation, Perspectives, Challenges and Strategies. Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China. P. 1-6.
Fageria NK (2002) Influence of micronutrients on dry matter yield and interaction with other nutrients in annual crops. Pesquisa Agropecuária Brasileira 37: 1765-1772. https://doi.org/10.1590/S0100-204X2002001200013
Horváth Á, Kiss T, Berki Z, Horváth ÁDK, Cseh A, Veisz O, Karsai I (2023) Effects of genetic components of plant development on yield-related traits in wheat (Triticum aestivum L.) under stress-free conditions. Frontiers in Plant Science 13: 1070410. https://doi.org/10.3389/fpls.2022.1070410
Liu X, Feng Z, Zhang S, Zhang J, Xiao Q, Wang Y (2006) Preparation and testing of cementing nano-sub nano composites of slow or controlled release of fertilizers. Scientia Agricultura Sinica 39: 1598-1604.
Mahajan P, Dhoke SK, Khanna SA (2011) Effect of nano-ZnO particle suspension on growth of mung (Vigna radiata) and gram (Cicer arietinum) seedlings using plant agar method. Journal of Nanotechnology 2011: 1-7. https://doi.org/10.1155/2011/696535
Manivasagaperumal R, Balamurugan S, Thiyagajan G, Sekar J (2011) Effect of Zinc on Germination, Seedling Growth and Biochemical Content of Cluster Bean (Cyamopsis tetragonoloba (L.) Taub). Current Botany 2(5): 11-15.
Maphosa Y, Jideani VA (2017) The Role of Legumes in Human Nutrition. In: Chávez-Mendoza C, Sánchez E (Eds.) Functional Food - Improve Health through Adequate Food. InTech. https://doi.org/10.5772/intechopen.69127
Marschner P (1995) Mineral Nutrition of Higher Plants. Second Edition. Academic Press, London. 889 pp. https://doi.org/10.1016/B978-012473542-2/50001-8
Mashayekhi P, Sharifi H (2022) Evaluation of several digestion methods in the extraction of heavy elements from different organic sources. Advanced Environmental Sciences 20(3): 17-34. https://doi.org/10.52547/envs.2021.1071
Mohammadpur M, Asl AN, Roshdi M, Jalili F, Rezadoost S (2025) Evaluation of strategies to reduce water use in pinto bean (Phaseolus vulgaris L.) cultivation under severe water deficit conditions. Agronomy Journal 117: e70096. https://doi.org/10.1002/agj2.70096
Panwar J, Jain N, Bhargava A, Akhtar MS, Yun YS (2012) Positive effect of ZnO nanoparticles on tomato plants: A step towards developing "nanofertilizers". In: Proceedings of the 3rd International Conference on Environmental Research and Technology. pp. 348-352.
Pesce S, Mamy L, Sanchez W, Artigas J, Bérard A, Betoulle S, Chaumot A, Coutellec MA, Crouzet O, Faburé J, Hedde M, Leboulanger C, Margoum C, Martin-Laurent F, Morin S, Mougin C, Munaron D, Nélieu S, Pelosi C, Leenhardt S (2025) The use of copper as plant protection product contributes to environmental contamination and resulting impacts on terrestrial and aquatic biodiversity and ecosystem functions. Environmental Science and Pollution Research International 32(6): 2830-2846. https://doi.org/10.1007/s11356-024-32145-z
Prasad TNV, Sudhakar P, Sreenivasulu Y, Latha P, Munaswamy V, Raja Reddy K, Sreeprasad TS, Sajanlal PR, Pradeep T (2012) Effect of nanoscale Zinc oxide particles on the germination, growth and yield of peanut. Journal of Plant Nutrition 35: 905-927. https://doi.org/10.1080/01904167.2012.663443
Seifi Nadergoli M, Yarnia M, Rahimzade Khoei F (2011) Effect of Zinc and Manganese and their application method on yield and yield components of common bean (Phaseolus vulgaris L. cv. Khomein). Middle-East Journal of Scientific Research 8(5): 859-865.
Shaviv A (2000) Advances in Controlled Release of Fertilizers. Advances in Agronomy 71: 1-49. https://doi.org/10.1016/S0065-2113(01)71011-5
Verma JP, Singh V, Yadav J (2011) Effect of Copper Sulphate on Seed Germination, Plant Growth and Peroxidase Activity of Mung Bean (Vigna radiata). International Journal of Botany 7(2): 200-204. https://doi.org/10.3923/ijb.2011.200.204
Victoria A, Nnebini DN (2025) A Systematic Review of Heavy Metals in Irrigation Water and Their Effects on Agricultural Soil Quality and Crop Production in Ghana. Journal of Geoscience and Environment Protection 13: 48-70. https://doi.org/10.4236/gep.2025.131004
Xu E, Liu Y, Gu D, Zhan X, Li J, Zhou K, Zhang P, Zou Y (2024) Molecular Mechanisms of Plant Responses to Copper: From Deficiency to Excess. International Journal of Molecular Sciences 25(13): 6993. https://doi.org/10.3390/ijms25136993
Zhou Q, Liu Z, Liu Y, Lin Q, Wang Z, Yang Y (2016) Relative abundance of chemical forms of Cu(II) and Cd(II) on soybean roots as influenced by pH, cations and organic acids. Scientific Reports 6: 36373. https://doi.org/10.1038/srep36373

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