SELECTIVE SOLID-PHASE EXTRACTION OF PHYTO–ECDYSTEROIDS FROM PLANT EXTRACTS BY USING MOLECULARLY IMPRINTED TiO2 NANOPARTICLES
Abstract
A sorbent molecularly imprinted with ecdystene, the most abundant phytoecdysteroid, was obtained by surface modification of TiO2 nanoparticles using hydrolysis of titanium(IV) n-butoxide. Ecdystene and its complex with 3-aminophenylboronic acid (APBA) were used as templates. The solid phase extraction (SPE) cartridges with the obtained sorbents were used for the group sorption of six phytoecdysteroids of sawwort (Serratula coronata). Compared with the silica-C18, the recovery of phytoecdysteroids with the imprinted sorbents is higher, and the recovery of matrix component lower. The analytes were adsorbed on the SPE cartridge, eluted by a mixture of acetone – acetonitrile – isopropanol – formic acid (10 : 45 : 45 : 1) and determined by HPLC–MS/MS technique. Sorption of phytoecdysteroids on the developed sorbent enabled their separation from the extract matrix. The sorbent based on the ecdystene – APBA complex was the most efficient: the recovery of phytoecdysteroids was 2–3 times higher those that with the non-imprinted sorbents. Obtained sorbents save their sorption properties during storage, this fact confirms the stability of molecular imprints.
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