REDOXOME FORMATION IN LEAVES OF WHEAT SEEDLINGS UNDER THE INFLUENCE OF OYSTER MUSH-ROOM EXTRACT
UDC 591.13:636.92+577.11
Abstract
Were studied the processes of oxidative modification of proteins (OMP), lipid peroxidation (LPO), the activity of cysteine proteinases (CP), superoxide dismutases (SOD), catalases (CAT), soluble peroxidases (PO), and the expression of some genes encoding them (SOD-1, CAT-1, POD and CP), the content of low molecular weight antioxidants (LMAO) (ascorbate, phylloquinones and free proline) and photosynthetic pigments in the leaves of week-old wheat seedlings cultivated using 10% and 100% extract based on spent oyster mushroom straw substrate (hereinafter referred to as extract). Plants were grown in both oligotrophic hydroponic and soil (gray forest) conditions. It was shown that under hydroponic conditions, in plants cultivated using 10% extract, LPO and OMP indicators were lower, and SOD activity, SOD-1 gene mRNA content, and ascorbate content were higher, and other indicators did not differ from control plants. In the experimental group using a 100% extract, all studied redox indicators were not significantly different from the control. In seedlings cultivated in soil conditions, the opposite result was revealed. Thus, in samples obtained using 100% extract, the level of LPO and OMP was lower, but at the same time the activity of SOD, CAT, CP, the expression of SOD-1, CAT-1, CP genes, the content of ascorbate, free proline and photosynthetic pigments were higher, and in leaves of plants grown using a 10% extract, all indicators did not differ from the control.
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