EXTRACTIVES OF PICEA OBOVATA LEDEB.
Abstract
The group and chemical composition of compounds extracted by solvents of various polarity from roots of Siberian Spruce (Picea obovata Ledeb.) have been investigated.
The Siberian Spruce roots were selected close toIrkutskcity in September 2015. The extractives were isolated from grinded roots (particle sizes are of 10–15 mm, humidity is 6,1%) by extraction with ethyl acetate at boiling temperature of solvent for an 4 hours, the hydromodule 1 : 10. Resinous substances were extracted by hexane from the ethyl acetate extracts a method of infusion for an 3 day, the hydromodule 1 : 100. The samples of roots after extraction by ethyl acetate dried at105 °Cuntil mass stabilization, then were obtained water-soluble matters extraction by distilled water at90 °Cwithin 4 h at the hydromodule 1 : 10.
The yields of the compounds extracted by ethyl acetate and water from roots and wood were close and were 1,9–2,1% (ethyl acetate) and 2,0–2,5% (water) of absolutely dry weight (in a.d.w.). The content of phenolic substances in ethyl acetate extract of roots was considerably more (more than in 2 times) than in an extract of wood.
The ethyl acetate extract of spruce root preliminarily treated with hexane, was separated by silica gel column using chloroform-acetone mixture as the eluent (with the increase of the latter content in mixture from 0 to 100%). The contents monomeric (0–50% (CH3)2СO inCHCl3), oligomeric (50% (CH3)2СO inCHCl3) and polymeric lignans (100% (CH3)2СO and MeOH) in an extract have been evaluated.
The comparative analysis of qualitative and quantitative chemical composition of roots and wood of Spruce was carried out. Researches have shown, that composition of phenolic extractives of roots and wood is very close. Differences are observed only on the relative content of compounds presented in extracts. The amount of dominant lignan – oxymatairesinol considerably above in wood of Spruce (in 1,7 times). The part of monomeric lignans in non-resinous ethyl acetate extracts of roots and wood was about 50% rel. and the part of the sum oligomeric and polymeric lignans was 35–40% rel.Downloads
Metrics
References
Babkin V.A. Khimiia rastitel'nogo syr'ia, 2014, no. 3, pp. 111–119. (in Russ.).
Kebbi-Benkeder Z., Colin F., Dumarcay S., Gerardin P. Annals of Forest Science, 2015, vol. 72, pp. 277–284.
Tranchuk N.V., Roshchin V.I. Khimiia rastitel'nogo syr'ia, 2015, no. 4, pp. 63–70. (in Russ.).
Levdanskii V.A., Butylkina A.I., Ivanchenko N.M., Kuznetsov B.N. Khimiia rastitel'nogo syr'ia, 2011, no. 1, pp. 93–99. (in Russ.).
Pietarinen S.P., Willfor S.M., Ahotupa M.O., Hemming J.E., Holmbom B.R. J. Wood Sci., 2006, vol. 52, pp. 436–444.
Fedorova T.E., Fedorov S.V., Babkin V.A. Khimiia rastitel'nogo syr'ia, 2015, no. 3, pp. 49–53. (in Russ.).
Neverova N.A., Levchuk A.A., Ostroukhova L.A., Medvedeva E.N., Onuchina N.A., Babkin V.A. Khimiia rastitel'nogo syr'ia, 2012, no. 4, pp. 91–100. (in Russ.).
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
