COMPARATIVE ANALYSIS OF CHEMICAL COMPOSITION AND NEMATOCIDAL ACTIVITY OF TAGETES PATULA (L., 1753) EXTRACTS IN EXPERIMENTS WITH THE SOIL NEMATODE CAENORHABDITIS ELEGANS (MAUPAS, 1900)

UDC 633.88+615.322+615.284+632.955

DOI_disc_logo https://doi.org/10.14258/jcprm.20250415442
Keywords: nematocides, Tagetes patula extracts, ethanol, chloroform, n-hexane, methyl tert-butyl ether, Caenorhabditis elegans

Abstract

Phytopathogenic nematodes have a negative impact on crop productivity. Currently, the main method of controlling these pests is the use of synthetic nematocides. Despite their high efficacy and ease of use, synthetic nematocides can pose a risk to both agricultural producers and consumers of crop products. An alternative to the use of chemical plant protection products can be the use of substances of natural origin, such as plant extracts. When using plant extracts to control agricultural pests, it should be borne in mind that the biological activity of extracts depends not only on the plant species, but also on the method of obtaining the extract. This work presents data on the physicochemical properties and biological activity of the extracts of Tagetes patula Lemon Drop variety obtained using four extractants of different polarity: ethanol, chloroform, n-hexane and methyl tert-butyl ether. The pH, total carotenoids, phenols, flavonoids and thiophenes content were determined for each extract. The biological activity of the extracts was studied in experiments with the free-living soil nematode Caenorhabditis elegans. All extracts had dose-dependent toxic effects on C. elegans organisms. The toxicity of the extracts depending on the solvent decreased in the series: chloroform > ethanol > n-hexane > methyl tert-butyl ether. The revealed differences in nematocidal activity of the extracts correlated with the content of phenols and carotenoids in them.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biographies

Tatyana Borisovna Kalinnikova, Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences

Candidate of Biological Sciences, Leading Researcher, Laboratory of Experimental Ecology

Dmitriy Aleksandrovich Terenzhev, Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS

Candidate of Chemical Sciences, Senior Researcher, Laboratory of Processing Plant Raw Materials for Organic Agriculture

Timur Gennadievich Belov, Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS

Researcher of the Laboratory for Processing of Plant Raw Materials for Environmentally Friendly Agriculture

Angelina Nikolaevna Menshova, Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS

Junior Researcher of the Laboratory for Processing of Plant Raw Materials for Environmentally Friendly Agriculture

Alsu Foatovna Gatiyatullina, Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences

Junior Researcher of the Laboratory of Experimental Ecology

Anastasia Vasilievna Egorova, Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences

Junior Researcher of the Laboratory of Experimental Ecology

Evgeny Nikolaevich Nikitin, Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS

Candidate of Chemical Sciences, Head of the Laboratory laboratory for processing plant materials for environmentally friendly agriculture

References

Ntalli N., Adamski Z., Doula M., Monokrousos N. Plants, 2020, vol. 9, pp. 1–20. https://doi.org/10.3390/plants9040429.

Chitwood D.J. Annual Review of Phytopathology, 2002, vol. 40, pp. 221–249. https://doi.org/10.1146/annurev.phyto.40.032602.130045.

Herrera-Estrella A., Casas-Flores S., Kubicek C.P. The Mycota IV: Envoronmental and microbial relationships. 3rd edition. Hedelberg: Springer, 2016, pp. 247–267. https://doi.org/10.1007/978-3-319-29532-9_13.

Abd-Elgawad M.M.M., Askary T.H. Egyptian Journal of Biological Pest Control, 2018, vol. 28, pp. 1–24. https://doi.org/10.1186/s41938-018-0080-x.

Soares F.E.de F., Sulfiate B.L., de Queiroz J.H. Agricultre and Natural Resources, 2018, vol. 52, pp. 1–8. https://doi.org/10.1016/j.anres.2018.05.010.

Wang K.-H., Hooks C.R., Ploeg A. Plant Disease, 2007, PD-35, pp. 1–7.

Hamaguchi T., Sato K., Vicente C.S.L., Hasegawa K. Biology Open, 2019, pp. 1–9. https://doi.org/10.1242/bio.038646.

Marahata S.P., Wang K.-H., Sipes B.S., Hooks C.R.R. Journal of Nematology, 2012, vol. 44, pp. 26–30.

Gabda D.D., Aglave B. Advances in Plant & Agriculture Research, 2015, vol. 2, pp. 213–219. https://doi.org/10.15406/apar.2015.02.00064.

Salehi B., Valussi M., Morais-Braga M.F.B., Carneiro J.N.P., Leal A.L.A.B., Coutinho H.D.M., Vitalini S., Kre-giel D., Antolak H., Sharifi-Rad M., Silva N.C.C., Yousaf Z., Martorell M., Iriti M., Carradori S., Sharifi-Rad J. Mole-cules, 2018, vol. 23, pp. 1–35. https://doi.org/10.3390/molecules23112847.

Kyo M., Miyauchi Y., Fujimoto T., Mayama S. Plant Cell Reports, 1990, vol. 9, pp. 393–397. https://doi.org/10.1007/BF00232407.

Fujimoto T., Kyo M., Miyauchi Y., Mayama S. Plant Tissue Culture Letters, 1990, vol. 7, pp. 177–180. https://doi.org/10.5511/plantbiotechnology1984.7.177.

Sturz A.V., Kimpinski J. Plant and Soil, 2004, vol. 262, pp. 241–249. https://doi.org/10.1023/B:PLSO.0000037046.86670.a3.

Nivsarkar M., Cherian B., Padh H. Current Science, 2001, vol. 81, pp. 667–672.

Ibrahim S.R.M., Abdallah H.M., El-Halawany A.M., Mohamed G.A. Phytochemistry Reviews, 2016, vol. 15, pp. 197–220. https://doi.org/10.1007/s11101-015-9403-7.

Xu L.-w., Chen J., Qi H.-y., Shi Y.-p. Chinese Herbal Medicine, 2012, vol. 4, pp. 103–117. https://doi.org/10.3969/j.issn.1674-6384.2012.02.004.

Sleigh J.N. Bioscience Horizons, 2010, vol. 3, pp. 29–39. https://doi.org/10.1093/biohorizons/hzq005.

Dent J.A. Biotechnology and Bioprocess Engineering, 2001, vol. 6, pp. 252–263. https://doi.org/10.1007/BF02931986.

Leung M.C.K., Williams P.L., Benedetto A., Au C., Helmcke K.J., Aschner M., Meyer J.N. Toxicological Sciences, 2008, vol. 106, pp. 5–28. https://doi.org/10.1093/toxsci/kfn121.

Brenner S. Genetics, 1974, vol. 77, pp. 71–94. https://doi.org/10.1093/genetics/77.1.71.

Sharonova N., Nikitin E., Terenzhev D., Lyubina A., Amerhanova S., Bushmeleva K., Rakhmaeva A., Fitsev I., Sinyashin K. Plants, 2021, vol. 10, article 1279. https://doi.org/10.3390/plants10071279.

Bushmeleva K., Vyshtakalyuk A., Terenzhev D., Belov T., Parfenov A., Sharonova N., Nikitin E., Zobov V. Plants, 2021, vol. 10, article 2458. https://doi.org/10.3390/plants10112458.

Tang J., Dunshea F.R., Suleria H.A.R. Foods, 2020, vol. 9, article 7. https://doi.org/10.3390/foods9010007.

Benea A., Ciobanu C., Cojocaru-Toma M., Ciobanu N. Moldovan Medical Journal, 2020, vol. 63, pp. 23–26. https://doi.org/10.5281/zenodo.4016806.

Katiki L.M., Ferreira J.F.S., Zajac A.M., Master C., Lindsay D.S., Chagas A.C.S., Amarante A.F.T. Veterinary Para-sitology, 2011, vol. 182, pp. 264–268. https://doi.org/10.1016/j.vetpar.2011.05.020.

Wang C., Bruening G., Williamson V.M. Journal of Chemical Ecology, 2009, vol. 35, pp. 1242–1251. https://doi.org/10.1007/s10886-009-9703-8.

Rocha T.L., Soll C.B., Boughton B.A., Silva T.S., Oldach K., Firmino A.A.P., Callahan D.L., Sheedy J., Silveira E.R., Carneiro R.M.D.G., Silva L.P., Polez V.L.P., Pelegrini P.B., Bacic A., Grossi-de-Sa M.F., Roessner U. Biotechnology Research and Innovation, 2017, vol. 1, pp. 87–100. https://doi.org/10.1016/j.biori.2017.10.003.

Devyatkina I.A., Zyubr T.P., Dudkin R.V., Bardakov A.I. Vestnik VGU. Seriya: Khimiya. Biologiya. Farmatsiya, 2005, no. 1, pp. 166–169. (in Russ.).

Zhang Q., Lin L., Ye W. Chinese. Medicine, 2018, vol. 13, article 20. https://doi.org/10.1186/s13020-018-0177-x.

Alara O.R., Abdurahman N.H., Ukaegbu C.I. Current Research in Food Science, 2021, vol. 4, pp. 200–214. https://doi.org/10.1016/j.crfs.2021.03.011.

D’Addabbo T., Tava A., Argentieri M.P., Biazzi E., Candido V., Avato P. Plants, 2022, vol. 11, article 2550. https://doi.org/10.3390/plants11192550.

Kyndt T., Nahar K., Haeck A., Verbeek R., Demeestere K., Gheysen G. Frontiers in Plant Science, 2017, vol. 8, article 951. https://doi.org/10.3389/fpls.2017.00951.

Li L.B., Xiao G.D., Xiang W., Yang X., Cao K.X., Huang R.S. Molecules, 2019, vol. 24, article 805. https://doi.org/10.3390/molecules24040805.

Gol'dshteyn I.P., Il'icheva Z.F., Slovokhotova N.A., Gur'yanova Ye.N., Kocheshkov K.A. Doklady AN SSSR, 1962, vol. 144, pp. 788–791. (in Russ.).

Liu T., Wu H., Jiang H., Zhang L., Zhang Y., Mao L. Journal of Agricultural and Food Chemistry, 2019, vol. 67, pp. 6160–6168. https://doi.org/10.1021/acs.jafc.9b01306.

Desmedt W., Mangelinckx S., Kyndt T., Vanholme B. Frontiers in Plant Science, 2020, vol. 11, article 602079. https://doi.org/10.3389/fpls.2020.602079.

Petrikovszki R., Tóth F., Nagy P.I. Journal of Nematology, 2023, vol. 55, article 20230037. https://doi.org/10.2478/jofnem-2023-0037.

Published
2025-11-20
How to Cite
1. Kalinnikova T. B., Terenzhev D. A., Belov T. G., Menshova A. N., Gatiyatullina A. F., Egorova A. V., Nikitin E. N. COMPARATIVE ANALYSIS OF CHEMICAL COMPOSITION AND NEMATOCIDAL ACTIVITY OF TAGETES PATULA (L., 1753) EXTRACTS IN EXPERIMENTS WITH THE SOIL NEMATODE CAENORHABDITIS ELEGANS (MAUPAS, 1900) // Chemistry of plant raw material, 2025. № 4. P. 204-215. URL: https://journal.asu.ru/cw/article/view/15442.
Issue
No 4 (2025): Online First
Section
Low-molecular weight compounds

Copyright (c) 2025 chemistry of plant raw material

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

The authors, which are published in this journal, agree to the following conditions:

1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .

2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.

3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.

Crossref
Scopus
Google Scholar
Europe PMC