BIOTECHNOLOGICAL PRINCIPLES OF THE INDUSTRIAL METHOD FOR THE PRODUCTION OF PHYCOERYTHRIN EXTRACT FROM THE BIOMASS OF THE RED MICROALGAE PORPHYRIDIUM PURPUREUM
UDC 582.273:573.7:574.6
Abstract
The red pigment B-phycoerythrin (B-PE), a component of the light- harvesting complex of the microalgae Porphyridium purpureum, has gained wide practical application in recent years in food, cosmetics, pharmacology, and biomedical research. The elaborated technologies for large-scale cultivation of P. purpureum and the high content of B- PE in its biomass are the basis for expanding the practical application of this pigment. The study was aimed to develop a method for obtaining aqueous B-PE extract from the biomass of red microalgae P. purpureum. The technological scheme for obtaining B- PE pigment from P. purpureum biomass was proposed, which includes the main stages: cultivation of microalgae, separation of the obtained biomass from the culture medium, destruction of cell walls of P. purpureum and B-PE extraction, identification, quantification and quality control of the obtained extract, storage of the aqueous extract of B-PE. The biotechnological principles of B- PE extract production from marine microalgae P. purpureum have been developed. Optimal conditions of microalgae culture cultivation, biomass storage, cell destruction and pigment extraction, as well as storage of the obtained B- PE extract were determined. The method of separation of P. purpureum biomass from the culture medium using a flow-type separator was tested and recommended. The advantages of the freezing method for long-term storage of the obtained biomass and aqueous extracts in terms of B-PE pigment stability have been experimentally proved. General recommendations for the establishment of pilot production of the pigment are given.
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