QUANTIFICATION OF TOTAL FLAVONOID CONTENT IN THE AERIAL PART AND IN THE TINCTURE OF IRIS LACTEA (IRIDACEAE)
Abstract
Iris lactea Pall.of Iridaceae Juss. family contains a complex of biologically active substances with antiviral, immunomodulatory, adaptogenic, antihypoxic, cardiostimulatory and antitumor properties, Thus, the herbal substance and dosage forms on its basis are promising in medicine.
The main groups of active substances in the aerial part of Iris Lactea are flavonoids and xanthones. There is a known method of chromatospectrophotometric quantification of total xanthone content calculated as alpisarin. Meanwhile, the development of a convenient method of quantification of total flavonoid content in the aerial part of Iris lactea and drugs on its basis is currently important.
The aims of this work are to develop a method of quantification of total flavonoid content calculated as apigenin in the aerial part of Iris lactea and in its tincture, using the differential spectrophotometry method according to the complexation reaction with aluminium chloride solution;to prove reliability and accuracy of this method for the analysis of Iris Lactea tincture in terms of the following factors: specificity, linearity, validity, precision, and repeatability.
As a result of verification data analysis, the developed method of quantification of total flavonoid content calculated as apigenin can be admitted for standardization of Iris Lactea herbal substance and tincture.
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