Aulacoseira capitalina sp. nov. (Bacillariophyta) from the Middle Miocene sediments of the Barguzin Valley, Baikal Rift Zone (Russia)

Аннотация

Using light and scanning electron microscopy, a new species Aulacoseira capitalina Titova et Usoltseva from the Middle Miocene deposits of the Barguzin Valley is described. It is shown that the new species is similar to other elliptical species in terms of elliptical valve face profile, diameter, deep ringleist, but differs in the shape of separating spines, the absence of ridge structure and the shape of rimoportulae. The finding of A. capitalina in the Middle Miocene deposits of the Barguzin Valley expands the range of distribution of elliptical species.

Corresponding author: Marina V. Usoltseva (usmarina@inbox.ru)

Academic editor: R. Yakovlev | Received 15 June 2022 | Accepted 5 October 2022 | Published 25 November 2022

http://zoobank.org/C33D6404-D768-4A10-B957-466A79E2E088

Citation: Titova LA, Hassan AI, Usoltseva MV (2022) Aulacoseira capitalina sp. nov. (Bacillariophyta) from the Middle Miocene sediments of the Barguzin Valley, Baikal Rift Zone (Russia). Acta Biologica Sibirica 8: 571–582. https://doi.org/10.14258/abs.v8.e35

Keywords

Aulacoseira capitalina, elliptical valves, fossil diatoms, Middle Miocene

Introduction

The Barguzin Valley is the largest intermountain depression located between the Barguzin and Ikat ridges to the northeast of the Svyatoy Nos peninsula and the Barguzin Bay of Lake Baikal (Fig. 1). It is one of the axial structures of the Baikal Rift Zone.

We started the study of diatoms from the Miocene–Pliocene deposits of the Barguzin Valley in 2012. Since then, diatoms from cores 532, 531 and 545 have been studied (Usoltseva et al. 2019; Hassan et al. 2019; Usoltseva et al. 2020).

It was shown that Aulacoseira Thwaites species dominated in all cores. Only one core (532) contained Aulacoseira with an elliptical valve. Previously, diatoms with an elliptical valve shape were not mentioned in the fossil deposits of the Baikal Region. Their findings are known from freshwater Miocene deposits of the submarine Yamato Rise, Japan Sea (Akiba et al. 1996; Burckle and Akiba 1978; Tsoy et al. 1985; Tsoy and Shastina 1999; Usoltseva and Tsoy 2010; Tsoy and Usoltseva 2016), North Korea (Iwahashi 1935), Oregon, USA (Van Landingham 1967), Japan (Morita et al. 1996; Tanaka et al. 2008; Tanaka and Nagumo 2011), and Primorye (Pushkar et al. 2019). The purpose of this work was to study elliptical Aulacoseira using light and scanning microscopy, to compare them with other species with elliptic valves and to describe a new species.

Figure 1.Location of the core 532.

Material and methods

The new species was found in the sample 94 m from core 532 taken on the southeast of Barguzin Valley by Baikal branch of Sosnovgeology "Urangeologorazvedka".

Preparation of samples for light microscopy and quantitative accounting was carried out according to the method described in [Grachev et al. 1998]. Cleaned valves were dried on cover slips and mounted in Naphrax (Naphrax Ltd., United Kingdom, refractive index = 1.74) and counted using light microscopy Axiovert 200 ZEISS LM (Carl Zeiss, Jena, Germany) equipped with a Pixera Penguin 600CL camera. Diatom frustules were broken by crushing a drop of the material between two cover glasses. The material was then placed on the stub for mounted on and coated with gold using a SDC 004 (BALZERS) ion sputter for 150 seconds at 10–15 mA. The sample was analysed using a SEM Quanta 200 (FEI Company, USA) at 21.5 kV and 10 mm working distance.

Results

Aulacoseira capitalina Titova et Usoltseva sp. nov. (Figures 2–5).

Type locality: Russia, Barguzin Valley

Material examined: Middle Miocene sediments in Barguzin valley, core 532 (53°44'28.1"N; 110°11'31.3"E).

Holotype: NS0046231 (Central Siberian Botanical Garden, Novosibirsk, Russia, Herbarium of Vascular Plants) Holotype specimen is illustrated in Figure 2D.

Isotypes: Slide № 532–94, SEM stubs 14047, 15818, 17547, 17162, 17164 deposited at Limnological Institute of SB RAS, Irkutsk, Russia.

Habitat: collected from sample 94 m, 532 core.

Etymology: New species is dedicated to the mother of one of the author Titova Kapitalina Anatolyevna.

Description: Valves cylindrical, elliptical valve face 9–18 μm in diameter in the apical axis and 9–16 μm in diameter in the transapical axis, 4.4–15 μm in height. The height/diameter ratio varies within 0.31–0.85. The ratio of diameter to apical and transapical axes is 1–1.3.

Mantle striae straight, 8–12 in 10 μm. Each stria comprises a single row of areolae, 8–10 in 10 μm. Linking spines wide spathulate. Separating spines short or elongated pointed with rounded ends. Ringleist deep. Ringleist aperture 3–6.7 μm wide. Rimoportulae (4–8) with straight stalk are embedded in the ringleist. The channels of rimoportulae penetrate through the ringleist and open into the first areola of the row.

A. capitalina f. capitalina Titova et Usoltseva. Valves are straight along the central axis.

A. capitalina f. curvata Titova et Usoltseva. Valves are curved along the central axis.

Differential diagnosis. A. capitalina is similar to other elliptical species in terms of elliptical valve face profile, diameter, deep ringleist, but differs in the shape of separating spines, the absence of ridge structure, and the shape of rimoportulae.

LM and SEM observation. LM observation shows that A. capitalina has an elliptical flat valve face without areolae (Figs 2A, 4B, 4C) or with areolar ring at the periphery (Figs 2B, 4A). The valves are variable in size (Fig. 2B–E) and in shape from narrow (Figs 2 A, D–H, J–P, 4 B–J) to broadly elliptical, almost round (Figs 2B, C, I, 4 A).

Figure 2.Aulacoseira capitalina sp. nov.: A, B – external views; C–P – internal views showing a ringleist (R), variability of aperture of ringleist in their shape. D – holotype. LM. Scale bar: 10 μm.

Frustules are short cylinders, straight (Figs 3A, B, F, 4I, J, L) or curved (Figs 3C–E, 4G, H, K, M) along the central axis. The longest chain found had eleven valves (Fig. 3C). The ringleist is very deep, 3.5–4.6 μm (Figs 2 C–P, 4 D–F). The shape of the ringleist aperture (3–6,7 μm) varies and may be oval (Figs 2D, E, H, N, 4F), wide and round (Figs 2B, C, F, M, O, P, 4D), small and round (Figs 2J, K, 4E) or uneven (Fig. 2D, G, I).

Figure 3.Aulacoseira capitalina sp. nov.: A – internal views; B–F – external views, showing different size of the valves. LM. Scale bar: 10 μm.

The collar is not broad but ribbed, 1.1–2.8 μm (Fig. 4G–M). Linking spines are broadly spathulate (Fig. 4H–L, O, Q), located between the rows of areoles, with a frequency of one spine per one row. Separating spines short or elongated pointed with rounded ends (Figs 3B, C, 4J, L–N, P). Round areolae located on the mantle in straight rows (Figs 3A–F, 4G–M).

Figure 4.Aulacoseira capitalina sp. nov.: A–Q – external views; A – valve face with areolar ring at the periphery; B, С – valve face without areolae; D–F – variability of aperture of ringleist in their shape; H–M – girdle view, showing valves with spines; N, P – separating spines; O, Q – linking spines. R – ringleist. SEM. Scale bar: A–M – 10 μm; N–Q – 1 μm.

Areolae of thick–walled cells seen from outside have 4–5 volae running to the centre (Fig. 5A), from inside – a bell–like plate (Fig. 5B, C, F) with radial perforations attaches to them. On some valves, you can see that they are completely covered inside with a plate (Fig. 5E) or the remains of this plate are visible (Fig. 5F, H, L). Rimopurtulae with straight stalk are embedded in the ringleist (Fig. 5C, D, G–L, O). The channels of rimoportulae straight or curved penetrate through the ringleist and open into the first areola of the row (Fig. 5F–H, M, N, P).

Figure 5.Aulacoseira capitalina sp. nov.: A – external view showing areolae velum; B–P – internal views showing areolae velum (B, C, E, F), rimoportulae position (C–P) and channel of rimoportula (E–H, M, N, P). V – velum, rch – rimoportulae channel, arrows – rimoportulae. SEM. Scale bar: D–N – 5 μm; A–С, O, P – 1 μm.

Discussion

Aulacoseira is a freshwater planktonic genus that has been widely distributed from the Eocene to nowadays. In most Aulacoseira species, the frustule is circular in valve view (Simonsen 1979). Elliptical valves have 5 taxa: Aulacoseira ovata Usoltseva et Tsoy, A. elliptica Tsoy et Usoltseva, A. hachiyaensis Tanaka, A. iwakiensis Tanaka et Nagumo and Melosira distans var. ovata. The features of the proposednew species A. capitalina and the other elliptical Aulacoseira taxa are shown in Table 1.

*data was given from published figures. a.a. – apical axis, t.a. – transapical axis, – no information.

Aulacoseira capitalina is similar to Melosira distans var. ovata in the valve face profile, in diameter and in deep ringleist, but differs in height, in number of areolae in 10 μm of a row and in the absence of areolae on the valve face. It is obvious that it is impossible to carry out complete comparison of these species as there is no information on elements of the frustules fine structure. We have seen only three schematic drawing in Iwahashi (1935).

A. capitalina is similar to A. ovata in the valve face profile, in linking spines, in deep ringleist, in absence of areolae on the some valve face or their location along the ring, in external opening of rimoportulae, but differs in greater variability in height and diameter, in lower density of areolae, in shape of separating spines, in ringleist aperture, in absence of ridge, in number and shape of rimoportulae.

A. capitalina is similar to A. elliptica in the valve face profile, in height and diameter, in areolae location on the valve face, in deep ringleist and aperture of the ringleist, but differs in lower density of areolae, in shape of separating spines, in absence of ridge, in number and shape of rimoportulae.

A. capitalina is similar to A. hachiyaensis in the valve face profile, in height and diameter, in number of areolae in 10 μm of a row, but differs in number of areolae in 10 μm of a row, in areolae location on the valve face, in shape of spines, in absence of ridge, aperture of the ringleist and in shape of rimoportulae.

A. capitalina is similar to A. iwakiensis in diameter, in absence of ridge, in shape of rimoportulae, but differs in height, in lower density of areolae, in areolae location on the valve face, in shape of spines, in ringleist aperture.

Thus, the elliptical valve face profile, diameter, deep ringleist, and thick valve walls are common to all elliptical Aulacoseira. These characters are probably ancient, since they are found in representatives of Aulacoseira from the Early Miocene deposits. The ridge structure found in A. ovata, A. elliptica, A. hachiyaensis can also be attributed to ancient characters.

The unique features of A. capitalina are the shape of the separating spines and the shape of the rimoportulae. Other characters are similar to other species that have a round valve face profile. For example, the velum in A. capitalina is similar to that of Aulacoseira baicalensis (K. Meyer) Simonsen and A. nyassensis (O. Müll) Simonsen. It is known that these species are endemic to Lakes Baikal and Nyasa. Previously, it was assumed that the characters of the velum in this species may have an adaptive function suited to the conditions in deep water (Crawford, Likhoshway, 2001).

Rimoportulae of A. capitalina are similar to that of Melosira californica (Ehrenbeg) Ralfs from Oregon (Fig. 23 in Likhoshway and Crawford 2001). They are represented by straight stalks that open into the first areola of the row. One more species, A. canadensis (Hustedt) Simonsen has rimoportulae such as stalk lying on ringleist. But, unlike the above species, the channels are diagonal and open below the first areola of the row with a small round opening. This type of rimoportulae is regarded as a feature of ancient Aulacoseira species (Likhoshway and Crawford 2001).

Species of Aulacoseira with an elliptical valve surface have been found in Early Miocene deposits of Japan and Japan Sea (Usolteva and Tsoy 2010). Thus, the elliptical Aulacoseira in this region may be Early Miocene markers. The age of the Korea deposits where Melosira distans var. ovata is still not well defined. The finding of A. capitalina in the Middle Miocene deposits of the Barguzin Valley expands the range of distribution of elliptical species.

Acknowledgements

The authors are grateful to S.V. Rasskazov (Institute of the Earth's Crust SB RAS) for organizing of the expedition and for taking samples from the cores of the Barguzin Valley. We thank the director of Central Siberian Botanical Garden (Novosibirsk, Russia) Viсtor Chepinoga for the opportunity to place the type specimen in the collection. The work is done within the State Assignments of Limnological Institute (0279–2021–0008). Microscopic studies were carried out in the Electron microscopy center of collective instrumental center “Ultramicroanalysis” Limnological Institute of the Siberian Branch of the Russian Academy of Sciences.

References

Akiba F, Tanimura Y, Tsoy I, Hiramatsu C (1996) Morphology of an extinct nonmarine diatom, Aulacoseira moisseevae n. sp. from Japan and two other related species with elliptic valves, and their taxonomic implication. In: Abstracts of the 14th international diatom symposium, Tokyo (Japan), September 2–8, 1996,1.

Burckle LN, Akiba F (1978) Implications of Late Neogene freshwater sediment in the sea of Japan. Geology 6: 123–127.

Grachev MA, Vorobyova SS, Likhoshway YeV, Goldberg EL, Ziborova GA, Levina OV, Khlystov OM (1998) A high resolution diatom record of the palaeoclimates of East Siberia for the last 2.5 my from Lake Baikal. Quaternary Sci. Rev. 17, 1101–1106.

Crawford RM, Likhoshway YeV (2001) The velum of species of the diatom genus Aulacoseira Thwaites. In: John J (Ed.) Proceedings of the 15th international diatom symposium, Perth, Australia, September 28–October 2, 1998. Koeltz Scientific Books, Koeningstein, 275–287.

Hassan A, Usoltseva МV, Rasskazov S, Chuvashova I, Titova L (2019) The first study of fossil diatom flora from Middle Miocene–Lower Pliocene lacustrine sediments in Barguzin valley, Baikal Rift Zone. Quarternary International 524: 24–30. https://doi.org/10.1016/j.quaint.2019.03.024

Iwahashi Y (1935) Freshwater centricae in Japan (Iv). Journal of japanese botany 11: 768–771. [In Japanese].

Likhoshway YeV, Crawford RM (2001) The rimoportula – a neglected feature in the systematics of Aulacoseira. In: Economou–Amilli A (Ed.) Proceedings of the 16th International Diatom Symposium, August 25–September 1, 2000. Amvrosiou Press, Athens, Greece, 33–48.

Morita R, Titova LV, Akiba F (1996) Oligocene–Early Miocene molluscs and diatoms from the Kitami–Tsubetsu area, Eastern Hokkaido, Japan. Sciens reports of the Tohoku University, second series (Geology)63 (2): 53–187.

Pushkar VS, Likhacheva OYu, Usoltseva MV (2019) Zonal diatom scale of the continental neogene in Primorye (most southern territory of the Russian Far East). International Journal on Algae 21(2): 163–176. https://doi:10.1615/InterJAlgae.v21.i2.60

Simonsen R (1979) The diatom system: ideas on phylogeny. Bacillaria 2, 9–71.

Tanaka H, Nagumo T, Akiba F. (2008) Aulacoseira hachiyaensis sp. nov., a new early Miocene freshwater fossil diatom from Hachiya Formation, Japan. In: Likhoshway Ye (Ed.) Proceedings of the 19th international diatom symposium, Irkutsk, August 28–September 3, 2006. Biopress Limited, Bristol, 115–123.

Tanaka H, Nagumo T. (2011) Aulacoseirai wakiensis sp. nov., a new elliptical Aulacoseira species from an early Miocene sediment, Japan. Diatom 27: 1–8.

Tsoy IB, Vashchenkova NG, Gorovaya MT, Terekhov YeP (1985) On finding of continental deposits on Yamato Rise. Pacific Geology 3: 50–55. [In Russian]

Tsoy IB, Shastina VV (1999) Siliceous micro–plankton of Neogene in the sea of Japan (Diatoms And Radiolarians). Dal’nauka, Vladivostok. 241 pp. [In Russian]

Tsoy IB, Usoltseva MV (2016) Miocene freshwater diatoms from the eastern slope of the submarine Ulleung plateau (Krishtofovich Rise) in the sea of Japan. Stratigraphy and geological correlation 24 (3): 276–293. [In Russian]. https://doi.org/10.1134/S0869593816020064

Usoltseva MV, Tsoy IB (2010) Elliptical species of the freshwater genus Aulacoseira in Miocene sediments from Yamato Rise (sea of Japan). Diatom Research. 25 (2): 397−415. https://doi.org/10.1080/0269249x.2010.9705859

Usoltseva MV, Titova LA., Hassan A, Chuvashova IS, Rasskazov SV (2019) Centric diatoms from paleolakes of the Baikal Rift Zone, Russia. Issues of modern algology 2 (20): 279–284. [In Russian]. https://doi.org/10.33624/2311–0147–2019–2(20)–279–284

Usoltseva МV, Hassan A, Rodionova EV, Titova LA., Chuvashova IS, Rasskazov SV (2020) The first finding of diatoms from the early Miocene lacustrine deposits of the Barguzin valley (Baikal Rift Zone). Limnology and freshwater biology 4: 752–754. https://doi.org/10.31951/2658–3518–2020–A–4–752

Van Landingham SL (1967) Paleoecology and microfloristics of Miocene diatomites. Nova Hedwigia. Beiheft26: 1–77.