PARTICIPATION OF POLYPHENOLS IN THE FORMATION OF FUNGICIDAL ACTIVITY AND RESISTANCE OF HELIANTHUS ANNUUS L. CALLUS CULTURES TO EXOMETABOLITES OF THE FUNGUS SCLEROTINIA SCLERO-TIORUM L.
UDC 574.577
Abstract
The effect of secondary metabolites, in particular polyphenols, on the resistance of callus tissues to the action of Sclerotinia sclerotiorum culture filtrate was studied. The studies were carried out on callus cultures obtained from explants isolated from sterile seedlings of Helianthus annuus L. It has been experimentally shown that under in vitro conditions, the content of soluble phenolic compounds sharply decreases in comparison with the original tissues and remains at a low level as it passes. However, when exposed to a stress factor (cultural filtrate of the fungus S. sclerotiorum), the accumulation of polyphenols increases. A direct correlation was established between the concentration of the cultural filtrate of the fungus S. sclerotiorum and the biosynthetic potential of callus cultures. With an increase in the concentration of cultural filtrate, the quantitative and qualitative content of polyphenols in the callus tissue increases. Specific histochemical studies. It is shown that polyphenols in sunflower plants are mainly localized in the integumentary, parenchymal and conductive tissues. As plants grow, the accumulation of polyphenols in plant tissues increases.
Downloads
Metrics
References
Zaprometov M.N. Fenol'nyye soyedineniya i ikh rol' v zhizni rasteniya: 56 Timiryazevskoye chteniya. [Phenolic com-pounds and their role in plant life: 56 Timiryazev Readings]. Moscow, 1996, 45 p. (in Russ.).
Alekseyeva G.M. et al. Farmakognoziya. Lekarstvennoye syr'ye rastitel'nogo i zhivotnogo proiskhozhdeniya. Pharma-cognosy. [Medicinal raw materials of plant and animal origin]. St. Petersburg, 2013, p. 378. (in Russ.).
Nosov A.M. Biotekhnologiya, 2010, no. 5, pp. 8–28. DOI: 10.1134/S000368381107009X. (in Russ.).
Zhurba O.V., Dmitriyev M.Ya. Lekarstvennyye, yadovityye i vrednyye rasteniya. [Medicinal, poisonous and harmful plants]. Moscow, 2002, 512 p. (in Russ.).
Brossa R., Casals I., Pintó-Marijuan M., Fleck I. Trees, 2009, vol. 23, no. 2, pp. 401–408. DOI: 10.1007/s00468-008-0289-5.
Kalashnikova Ye.A., Cherednichenko M.Yu., Zaytseva S.M., Kochiyeva Ye.Z., Khaliluyev M.R., Karsunkina N.P. La-boratornyy praktikum po biotekhnologii rasteniy (uchebnoye posobiye). [Laboratory workshop on plant biotechnology (textbook)]. Moscow, 2019, 240 p. (in Russ.).
Nguyen Thanh Hai. Izvestiya TSKHA, 2007, no. 2, pp. 116–124. (in Russ.).
Berestetskiy O.A. Izucheniye fitotoksicheskikh svoystv mikroskopicheskikh gribov. Metody eksperimental'noy mikologii. [Study of phytotoxic properties of microscopic fungi. Methods of experimental mycology]. Kyiv, 1982, pp. 321–333. (in Russ.).
Zaprometov M.N. Fenol'nyye soyedineniya i metody ikh issledovaniya. Biokhimicheskiye metody v fiziologii rasteniy. [Phenolic compounds and methods for their study. Biochemical methods in plant physiology]. Moscow, 1971, pp. 185–197. (in Russ.).
Soukupova J., Cvikrova M., Albrechtova J., Rock B.N., Eder J. New Phytol., 2000, vol. 146, pp. 403–414. DOI: 10.1046/j.1469-8137.2000.00666.x.
Araslanova N.M., Ivebor M.V., Saukova S.L., Antonova T.S. Maslichnyye kul'tury, 2014, no. 1 (157–158), pp. 124–129. (in Russ.).
Zagoskina N.V., Goncharuk E.A., Alyavina A.K. Russian Journal of Plant Physiology, 2007, vol. 54, pp. 237–243. DOI: 10.1134/S1021443707020124.
Hassan S., Mathesius U. Journal of experimental botany, 2012, vol. 63, no. 9, pp. 3429–3444. DOI: 10.1093/jeb/err430.
Schneider-Müller S., Kurosaki F., Nishi A. Physiological and Molecular Plant Pathology, 1994, vol. 45, no. 2, pp. 101–109. DOI: 10.1016/S0885-5765(05)80069-4.
Kalashnikova Ye.A., Zaytseva S.M., Doan Thu Thuy, Kirakosyan R.N. Aktual'nyye voprosy veterinarnoy biologii, 2018, no. 2(38), pp. 39–45. (in Russ.).
Kalashnikova Ye.A., Zaytseva S.M., Doan Thu Thuy, Kirakosyan R.N. Mezhdunarodnyy nauchno-issledovatel'skiy zhurnal, 2020, no. 6–2(96), pp. 6–11. DOI: 10.23670/IRJ.2020.96.6.039. (in Russ.).
Zaprometov M.N., Nikolayeva T.N. Fiziologiya rasteniy, 2003, vol. 50, no. 5, pp. 699–702. (in Russ.).
Jus Laoué J., Fernandez C., Ormeño E. Plants, 2022, vol. 11, no. 2, p. 172. DOI: 10.3390/plants11020172.
Kalashnikova Ye.A., Zaytseva S.M., Doan Thu Thuy, Kirakosyan R.N. Voprosy biologicheskoy, farmatsevticheskoy i meditsinskoy khimii, 2019, vol. 22, no. 5, pp. 48–54. DOI: 10.29296/25877313-2019-05-09. (in Russ.).
Dubravina G.A., Zaytseva S.M., Zagoskina N.V. Fiziologiya rasteniy, 2005, vol. 52, no. 5, pp. 755–762. DOI: 10.1007/s11183-005-0100-z. (in Russ.).
Sharma A., Shahzad B., Rehman A., Bhardwaj R., Landi M., Zheng B. Molecules, 2019, vol. 24, no. 13, article 2452. DOI: 10.3390/molecules24132452.
Lattanzio V., Lattanzino V.M.T., Cardinali A. Phytochemistry: Advances in research, 2006, vol. 661, no. 2, pp. 23–67.
Mar'in A.A., Kolomiyets N.E. Fundamental'naya i klinicheskaya meditsina, 2017, vol. 2, no. 4, pp. 45–55. DOI: 10.23946/2500-0764-2017-2-4-45-55. (in Russ.).
Kurkin V.A. Fenilpropanoidy – perspektivnyye prirodnyye biologicheski aktivnyye soyedineniya. [Phenylpropanoids are promising natural biologically active compounds]. Samara, 1996, pp. 31–37. (in Russ.).
Zengqi L., Tiexin T., Shejian L., Xiping N., Mei B., Hong W. American journal of Plant Sciences, 2012, vol. 2012, pp. 551–558. DOI: 10.4236/ajps.2012.34066.
Brunetti С., Fini А., Sebastiani F., Gori A., Tattini M. Frontiers in Plant science, 2018, vol. 9, article 1042. DOI: 10.3389/fpls.2018.01042.
![](http://journal.asu.ru/public/journals/1/article_11025_cover_ru_RU.png)
Copyright (c) 2023 chemistry of plant raw material
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.