PARTICIPATION OF POLYPHENOLS IN THE FORMATION OF FUNGICIDAL ACTIVITY AND RESISTANCE OF HELIANTHUS ANNUUS L. CALLUS CULTURES TO EXOMETABOLITES OF THE FUNGUS SCLEROTINIA SCLERO-TIORUM L.
UDC 574.577
Abstract
The effect of secondary metabolites, in particular polyphenols, on the resistance of callus tissues to the action of Sclerotinia sclerotiorum culture filtrate was studied. The studies were carried out on callus cultures obtained from explants isolated from sterile seedlings of Helianthus annuus L. It has been experimentally shown that under in vitro conditions, the content of soluble phenolic compounds sharply decreases in comparison with the original tissues and remains at a low level as it passes. However, when exposed to a stress factor (cultural filtrate of the fungus S. sclerotiorum), the accumulation of polyphenols increases. A direct correlation was established between the concentration of the cultural filtrate of the fungus S. sclerotiorum and the biosynthetic potential of callus cultures. With an increase in the concentration of cultural filtrate, the quantitative and qualitative content of polyphenols in the callus tissue increases. Specific histochemical studies. It is shown that polyphenols in sunflower plants are mainly localized in the integumentary, parenchymal and conductive tissues. As plants grow, the accumulation of polyphenols in plant tissues increases.
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