INFLUENCE METHYL JASMONATE ON PRODUCTION OF ECDYSTEROIDS FROM HAIRY ROOTS OF SILENE LINICOLA C.C.GMELIN
Abstract
Hairy root cultures are being considered as promising system for producing valuable second metabolites. These genetically transformed root cultures are characterized by high growth rate, genetic stability and growth in hormone free media. Using A. rhizogenes-mediated transformation method (strain А-4), we have obtained hairy root cultures of the ecdysteroid-containing species of Silene linicola. HPLC analysis of the sample studied revealed that 20-hydroxyecdysone (20E), turkesterone and polypodin B were synthesized under the specified experimental conditions. Application of methyl jasmonate at a concentration of 100 μM resulted in stimulation of 20E biosynthesis (up 74%) after 3 days of cultivation and turkesterone up to 35% at 6 days. It was noted that total ecdysteroid content in sample tested varied: turkesterone from 25 to 60%, and 20E from 8 to 30%. At the same time the level of 20E biosynthesis decreased from 0.023 to 0.014% in the samples without methyl jasmonate treatment. Hairy root lines of S. linicola with different responses to the presence of elicitors in the culture medium can be used to study the pathways of ecdysteroid biosynthesis.
Downloads
Metrics
References
http:ecdybase.org/
Zibareva L., Volodin V., Saatov Z., Savchenko T., Whiting P., Lafont R., Dinan L. Distribution of phytoecdysteroids in the Caryophyllaceae // Phytochemistry. 2004. Vol. 64. Pp. 499–517. DOI: 10.1016/S0031-9422(03)00376-5
Зибарева Л.Н., Волкова О.В., Морозов С.В., Черняк Е.И. Фитоэкдистероиды корней Silene frivaldszkyana Hampe // Химия растительного сырья. 2017. №1. С. 71–75. DOI: 10.14258/jcprm.2017011416
Зибарева Л.Н., Еремина В.И. Динамика содержания экдистероидов в видах Silene // Раст. ресурсы. 1996. Т. 32, вып. 1-2. С. 106–110.
Зибарева Л.Н., Еремина В.И., Иванова Н.А. Новые экдистероидоносные виды рода Silene L. и динамика содержания в них экдистерона // Раст. ресурсы. 1997. Т. 33, вып. 3. С. 73–76.
Giri A., Narasu M. L. Transgenic hairy roots: recent trends and applications // Biotechnology Advances. 2000. Vol. 18. Pp. 1–22. DOI:10.1016/S0734-9750(99)00016-6
Kim Y., Wyslouzil B.E., Weathers P.J. Secondary metabolism of hairy root cultures in bioreactors // In vitro Cell. Dev. Bio.l–Plant. 2002. Vol. 38. Pp. 1–10. DOI: 10.1079/IVP2001243
Georgiev M.I., Weber J., Maciuk A. Bioprocessing of plant cell cultures for mass production of targeted compounds // Appl. Microbiol. Biotechnol. 2009. Vol. 83. Pp. 809–823. DOI: 10.1007/s00253-009-2049-x
Shabania L., Ehsanpoura A. A., Asgharib G., Emamib J. Glycyrrhizin production by in vitro cultured Glycyrrhiza glabra elicited by methyl jasmonate and salicylic acid // Russian Journal of Plant Physiology. 2009. Vol. 56, №. 5. Pp. 621–626. DOI: 10.1134/S1021443709050069
Martin K.P., Sabovljevic A., Madassery J. High-frequency transgenic plant regeneration and plumbagin production through methyl jasmonate elicitation from hairy roots of Plumbago indica L. // J. Crop Sci. Biotech. 2011. Vol. 14, issue 3. Pp. 205–212. DOI: 10.1007/s12892-010-0123-7
Chodisetti B., Rao K., Gandi S., Giri A. Gymnemic acid enhancement in the suspension cultures of Gymnema sylvestre by using the signaling molecules–methyl jasmonate and salicylic acid // In Vitro Cell. Dev. Biol.–Plant. 2015. Vol. 51. Pp. 88–92. DOI: 10.1007/s11627-014-9655-8
Ram M., Prasad K. V., Singh S. K., Hada B. S., Kumar S. Influence of salicylic acid and methyl jasmonate elicitation on anthocyanin production in callus cultures of Rosa hybrida L. // Plant Cell Tiss. Organ Cult. 2013. Vol. 113. Pp. 459-467. DOI: 10.1007/s11240-013-0287-1
Cheng D.M., Yousef G.G., Grace M.H., Rogers R.B., Gorelick-Feldman J., Raskin I., Lila M.A. In vitro production of metabolism-enchancing phytoecdysteroids from Ajuga turkestanica // Plant Cell Tiss. Organ. Cult. 2008. Vol. 93. Pp. 73–83. DOI: 10.1007/s11240-008-9345-5
Schmelz E.A., Grebenok R.J., Ohnmeiss T.E., Bowers W.S. Phytoecdysteroid turnover in spinach: Long-term stability supports a plant defense hypothesis // J. Chem. Ecol. 2000. Vol. 26. Pp. 2883–2896. DOI: 10.1023/A:1026454213510
Володин В.В., Володина С.О., Чадин И.Ф., Пылина Я.И., Бачаров Д.С., Джумырко С.Ф., Бутенко Л.И. Состав экдистероидов в дикорастущих и культивируемых растениях Serratula quinquefolia Bieb. ex Willd // Вестник ИБ. 2010. №4. С. 29–34.
Филлипова В.Н., Володина С.О., Смоленская И.Н. Экдистероиды в культурах клеток Serratula coronata и Ajuga reptans // Химия растительного сырья. 2002. №1. С. 69-80.
Erst A.A., Zheleznichenko T.V., Badulina A.A., Zibareva L.N., Kovzunova O.V. Biosynthesis of phytoecdysteroids in the hairy root culture of Silene linicola C.C. Gmelin // Plant Cell Biotechnology and Molecular Biology. 2016. 17, issue 7-8. Pp. 326–334.
Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture // Physiol Plantarum. 1962. Vol. 15, issue 2. Pp. 473–497.
Gamborg O. L., Eveleigh D. E. Culture methods and detection of glucanases in cultures of wheat and barley // Can J Biochem. 1968. Vol. 46, issue 5. Pp. 417–421.
Van Furden B., Humburg A., Fuss. E. Influence of methyl jasmonate on podophyllotoxin and 6-methoxypodophyllotoxin accumulation in Linum album cell suspension cultures // Plant Cell Rep. 2005. Vol. 24. Pp. 312–317. DOI: 10.1007/s00299-005-0954-8
Lee E.J., Park S.Y., Paek K.Y. Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors // Plant Cell, Tiss. Organ Culture. 2015. Vol. 120, issue 1. Pp. 1–10. DOI: 10.1007/s11240-014-0567-4
Зибарева Л.Н. Фитоэкдистероиды растений семейства Сaryophyllaceae. 2012. Saarbrüсken, Germany – LAP Lambert Academic Publishing GmbH&Co. 195 с.
Gizatullina Z.Z., Gagelgans A.I. and Syrov V.N. (1994) Ecdysterone, turkesterone and nerobol alter thymocyte energy metabolism. Doklady Akademii Nauk Respubliki Uzbekistan (10), 49-52 [in Russian, with an English abstract].
Syrov V. N. and Kurmukov A. G. (1976) Anabolic properties of the phytoecdysone turkesterone and turkesterone tetraacetate in experiments on male rats. Problemy Endokrinologii - Moskva 22(3), 107- 112 [in Russian].
Bourgaud F., Bouque V., Guckert A. Production of flavlonoids by Psoralea hairy root cultures // Plant Cell Tiss Organ. Cult. 1999. Vol. 56. Pp. 97–104.
Jittayasothorn Y., Yang Y., Chen S., Wang X., Zhong Y. Influences of Agrobacterium rhizogenes strains, plant genotypes, and tissue types on the induction of transgenic hairy roots in Vitis species // Vitis. 2011. Vol. 50, № 3. Pp. 107–114.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
