THE EFFECT OF GROWING CONDITIONS ON THE QUALITATIVE AND QUANTITATIVE INDICATORS OF CHLORELLA VULGARIS
UDC 636.086.783
Abstract
The complex effect of two factors (the optimum ratio of nutrients of the medium and the electrostatic field) on the qualitative and quantitative indicators of Chlorella vulgaris was investigated. It is shown that the use of a medium with optimal concentrations of mineral substances and an electrostatic field with a voltage of 15 kV and an exposure time of 72 hours has a positive effect on growth rates, the size of microalgae cells and their viability, contributes to obtaining a culture with a density of 50 mln cells / ml 18 hours faster in comparison with the cultivation of generally accepted methods. It has been established that when two factors are influences on the cells of the Chlorella a mutagenic effect is not observed. Analysis of the microalgae culture grown under the influence of the studied factors showed that its chemical composition was consistent and with several indicators exceeded that of chlorella cultivation according to classical technologies. It was revealed that the activity of catalase and superoxide dismutase of microalgae grown in optimal conditions of the nutrient medium and the electrostatic field, significantly exceeded similar values of Chlorella vulgaris, which was not subjected to electrostatic stimulation. The high activity of the studied enzymes in Chlorella cells is shown in comparison with higher plants – components of bio-antioxidant preparations, such as Amaranthus paniculatus L. and Nicotiana tabacum L. According to the results of the toxicological study the absence of the content in Chlorella of the main poisonous substances for body animals and humans (mercury, arsenic) has been established. As a result of the research, it was proposed to use the established optimal parameters of both factors in the cultivation of C. vulgaris.
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