DEVELOPMENT AND VALIDATION OF COMPLEXOMETRIC METHODS FOR THE DETERMINATION OF CAL-CIUM AND MAGNESIUM IN MEDICINAL PLANT MATERIALS (ON THE GRASS PERSICARIA MACULOSA GRAY.)
UDC 615.07
Abstract
The relevance of the study of the mineral composition of plant objects is undoubted. In plant organisms, macro and micronutrients are present in an easily digestible form. The macroelements most accumulated in plants include calcium and magnesium, whose sources are members of the family Polygonaceae Juss, in particular the genus Persicaria Mill. The aim of the work was to develop a technique for quantitative complexometric determination of calcium and magnesium in plant raw materials after the burning procedure and its validation.
As a result, optimal conditions for the quantitative determination of calcium and magnesium in plant objects were experimentally selected using the example of Persicaria maculosa Gray. mountaineer grass using complexometric titration after ashing and calcination. For the determination of calcium, the optimum pH value is 11–12, the acidic indicator is dark blue – chromium, the optimum pH for magnesium is 8–9, the indicator is pyrocatechol violet. The calcium content in the plant varies from 0.12% to 0.58%, the amount of magnesium is about 0.02%. The relative error of the mean determination with a 95% probability was no more than 1.60% and no more than 1.84% when determining calcium and magnesium, respectively. Studies have shown the validity of the developed methodology for indicators of authenticity, precision (convergence, reproducibility), specificity, linearity.
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Listov S.A., Perov N.V., Arzamastsev A.P. Farmatsiya, 1990, no. 2, pp. 19–25. (in Russ.).
Sorokina A.A., Skalozubova T.A., Marakhova A.I. Farmatsiya, 2013, no. 2, pp. 5–8. (in Russ.).
Trineyeva O.V., Slivkin A.I. Khimiya rastitel'nogo syr'ya, 2015, no. 1, pp. 101–106. DOI: 10.14258/jcprm.201501291. (in Russ.).
Kaysinova R.Z., Neyelova O.V. Uspekhi sovremennogo yestestvoznaniya, 2011, no. 8, p. 225. (in Russ.).
Swaminathan R. Clinical Biochemist Reviews, 2003, vol. 24 (2), pp. 47–66.
Saris N.E., Mervaala E., Karppanen H., Khawaja J.A., Lewenstam A. Clinica Chimica Acta, 2000, vol. 294, pp. 1–26.
Gromova O.A., Kalacheva A.G., Torshin I.Yu., Yudina N.V., Yegorova Ye.Yu., Grishia T.R., Semenov V.A., Prozo-rova N.V., Sukhanova T.Yu., Belinskaya A.Yu. Arkhiv vnutrenney meditsiny, 2014, no. 3, pp. 6–10. (in Russ.).
Kudrin A.V., Skal'nyy A.V., Zhavoronkov A.A. Immunofarmakologiya mikroelementov. [Immunopharmacology of trace elements]. Moscow, 2000, 537 p. (in Russ.).
Perova I.B., Eller K.I.,Mal'tseva A.A., Chistyakova A.S., Slivkin A.I., Sorokina A.A. Farmatsiya, 2017, vol. 66, no. 2, pp. 15–19. (in Russ.).
Perova I.B., Eller K.I., Mal'tseva A.A., Chistyakova A.S., Slivkin A.I., Sorokina A.A. Farmatsiya, 2017, vol. 66, no. 5, pp. 27–30. (in Russ.).
Abdykalikova K.A., Islyambekova A.T. Biologicheskoye raznoobraziye aziatskikh stepey: materialy 2-y mezhdunarod-noy konferentsii, 5-6 iyunya 2012 g. [Biological diversity of Asian steppes: materials of the 2nd international conference, June 5-6, 2012]. Kostanay, 2012, pp. 174–177. (in Russ.).
Luksha Ye.A., Pogodin I.S., Ivanova Ye.V. Butlerovskiye soobshcheniya, 2015, vol. 41, no. 3, pp. 103–108. (in Russ.).
Mal'tseva A.A., Chistyakova A.S., Sorokina A.A., Slivkin A.I. Farmatsiya, 2016, no. 2, pp. 14–18. (in Russ.).
Chistyakova A.S. Farmakogosticheskoye issledovaniye travy gortsa pochechuynogo: dis. … kand. farm. nauk. [Phar-macogostical study of the herb of highlander pochechuyny: dis. ... Cand. farm. sciences]. Moscow, 2017, 200 p. (in Russ.).
Patent 2605855 (RU). 2016. (in Russ.).
Dzidakhanova T.A., Agayeva F.A. Mezhdunarodnyy studencheskiy nauchnyy vestnik, 2016, no. 3, URL: http://www.eduherald.ru/ru/article/view?id=15090. (in Russ.).
Khoang Chin' Ngok, Tkhi Fam Uiyen, An' Le Tkhi Kim, Trushcheko M.N., Buloychik Zh.I. Vestnik BGU. Ser. 1, 2014, no. 1, pp. 31–36. (in Russ.).
Patent 2466387 (RU). 2012. (in Russ.).
Gosudarstvennaya farmakopeya Rossiyskoy Federatsii: 14 izd. [State Pharmacopoeia of the Russian Federation: 14th ed.]. Moscow, 2018, 7019 p. (in Russ.).
Gosudarstvennaya farmakopeya Rossiyskoy Federatsii: 12 izd. [State Pharmacopoeia of the Russian Federation: 12th ed.]. Moscow, 2008, vol. 1, 704 p. (in Russ.).
Rukovodstvo po instrumental'nym metodam issledovaniy pri razrabotke i ekspertize kachestva lekarstvennykh prepara-tov [Guidance on instrumental research methods in the development and examination of the quality of medicinal prod-ucts], ed. S.N. Bykovsky, I.A. Vasilenko, M.I. Kharchenko, A.B. Belov, I.E. Shokhin, E.A. Dorina. Moscow, 2014, 656 p. (in Russ.).
Yevseyeva O.S., Andreyeva O.A., Oganesyan E.T. Nauchnyye vedomosti Belgorodskogo gosudarstvennogo universi-teta, 2013, no. 25 (168), pp. 55–60. (in Russ.).
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