BIOCATALYTIC PROPERTIES OF IMMOBILIZED INULINASЕ

  • Ирина (Irina) Викторовна (Viktorovna) Шкутина (Shkutina) Voronezh State University, Department of Chemistry, Universitetskaya pl.,1, Voronezh, 394006 Email: irn55@mail.ru
  • Ольга (Ol'ga) Федоровна (Fedorovna) Стоянова (Stoyanova) Voronezh State University, Department of Chemistry, Universitetskaya pl.,1, Voronezh, 394006 Email: common@anchem.vsu.ru
  • Владимир (Vladimir) Федорович (Fedorovich) Селеменев (Selemenev) Voronezh State University, Department of Chemistry, Universitetskaya pl.,1, Voronezh, 394006 Email: common@anchem.vsu.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.201501806
Keywords: inulinasе, Stirosorb, immobilization, catalytic activity, acid, thermic inactivation, inactivation constant

Abstract

The heterogeneous biocatalyst based on inulinasе immobilized on the non-ionogenic sorbent of Stirosorb was proposed. Investigation of thermic and acid inactivation of free and immobilized inulinasе was carried out. The corresponding values of the inactivation constants were rated. Increase of thermal stability of the immobilized ferment compared to the free one was exposed. Possibility of use of the immobilized ferment ten cycles long during the reaction of inulin hydrolysis was determined.

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Author Biographies

Ирина (Irina) Викторовна (Viktorovna) Шкутина (Shkutina), Voronezh State University, Department of Chemistry, Universitetskaya pl.,1, Voronezh, 394006
доцент кафедры аналитической химии, кандидат биологических наук, тел.: (473) 2208-932, e-mail: irn55@mail.ru
Ольга (Ol'ga) Федоровна (Fedorovna) Стоянова (Stoyanova), Voronezh State University, Department of Chemistry, Universitetskaya pl.,1, Voronezh, 394006
доцент кафедры аналитической химии, кандидат химических наук, тел.: (473) 2208-932
Владимир (Vladimir) Федорович (Fedorovich) Селеменев (Selemenev), Voronezh State University, Department of Chemistry, Universitetskaya pl.,1, Voronezh, 394006
заведующий кафедрой аналитической химии, профессор, доктор химических наук, Заслуженный деятель науки РФ, тел.: (473) 2208-932, e-mail: common@anchem.vsu.ru

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Published
2015-01-27
How to Cite
1. Шкутина (Shkutina)И. (Irina) В. (Viktorovna), Стоянова (Stoyanova)О. (Ol’ga) Ф. (Fedorovna), Селеменев (Selemenev)В. (Vladimir) Ф. (Fedorovich) BIOCATALYTIC PROPERTIES OF IMMOBILIZED INULINASЕ // chemistry of plant raw material, 2015. № 1. P. 141-145. URL: http://journal.asu.ru/cw/article/view/806.
Issue
No 1 (2015)
Section
Low-molecular weight compounds

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