СHROMATOGRAPHIC ANALYSIS OF СYCLOSIVERSIOSIDE F
UDC 615.322
Abstract
The plants Astragalus pterocephalus growing in Uzbekistan are a source of triterpene glycosides. The main triterpene glycoside, in terms of content, is a cycloartan glycoside – cyclosiversioside F. To obtain an individual biologically active compound cyclosiversioside F with 95% purity, a proposed method involves extraction with methanol, concentration and dilution with an equal volume of water, then followed by a sequential extraction from the aqueous extract with chloroform, ethyl acetate and butanol.
Then a chromatographic separation of the purified amount of extractives on a column with silica gel, isolation of the substance and precipitation from a solvent system must be performed, followed by recrystallization and drying. The optimal conditions for the isolation and separation of the amount of extractive substances have been developed in order to obtain an individual glycoside.
Cyclosiversioside F was authenticated by TLC in comparison with an authentic sample. Quantitative analysis of the glycoside was carried out by HPLC. The purity of cyclosiversioside F was confirmed by taking 1H and 13C NMR spectra.
Downloads
Metrics
References
Sukhikh A.S., Kuznetsov P.V. Meditsina v Kuzbasse, 2009, no. 4, pp. 3–12. (in Russ.).
Korovin Ye.P. Vvedenskiy A.V. Flora Uzbekistana. [Flora of Uzbekistan]. Tashkent, 1955, vol. 3, 684 p. (in Russ.).
Agzamova M.A., Isaev I.M. Chemistry of Natural Compounds, 2016, vol. 52, pp. 501–502. DOI: 10.1007/s10600-016-1687-3.
Agzamova М.А., Isaev М.I., Gorovits M.B., Abubakirov N.K. Chemistry of Natural Compounds, 1986, vol. 22, no. 1, pp. 115–116. DOI: 10.1007/BF00574606.
Agzamova M.A., Isaev M.I., Mal'tsev I.I., Gorovits M.B., Abubakirov N.K. Chemistry of Natural Compounds, 1988, pp. 755–756. DOI: 10.1007/BF00598208.
Agzamova M.A., Isaev I.M., Ibragimov T.F. Chemistry of Natural Compounds, 2014, vol. 50, no. 5, pp. 959–960. DOI: 10.1007/s10600-014-1133-3.
Agzamova M.A., Isaev M.I. Hacettepe University J. of Faculty of Pharmacy, 1999, vol. 19, no. 1, pp. 45–55.
Zhang L., Liu Q., Lu L., Zhao X., Gao X., Wang Y. J. Pharmacol. Exp. Ther., 2011, vol. 338, no. 2, pp. 485–491. DOI: 10.1124/jpet.111.180992.
Luo H.M., Dai R.H., Li Y. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1995, vol. 15, pp. 707–709.
Chen L.X., Liao J.Z., Guo W.Q. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1995, vol. 15, pp. 141–143.
Patent 57165400 (JP). 1983.
Bedir Е., Pugh N., Calis I., Pasco D.S., Khan I.A. Biological and Pharmaceutical Bulletin, 2000, vol. 23, no. 7, pp. 834–837. DOI: 10.1248/bpb.23.834.
Tsaruk A.V., Khushbaktova Z.A., Syrov V.N., Agzamova M.A., Kasimova G.M. Farmatsevticheskiy zhurnal, 2008, no. 1, pp. 72–75. (in Russ.).
Tsaruk A.V., Khushbaktova Z.A., Mamedova R.P., Syrov V.N. Farmatsevticheskiy zhurnal, 2007, no. 4, pp. 93–95. (in Russ.).
Khushbaktova Z.A., Agzamova M.A., Syrov V.N. et al. Chemistry of Natural Compounds, 1994, vol. 30, pp. 469–473. DOI: 10.1007/BF00630402.
Khushbaktova Z.A., Syrov V.N. Ukrainskiy biokhimicheskiy zhurnal, 1993, vol. 65, pp. 71–75. (in Russ.).
Tsaruk A.V., Iskenderov D.A., Agzamova M.A., Khushbaktova Z.A., Syrov V.N., Isayev M.I. Khimiko-farmatsevticheskiy zhurnal, 2010, no. 1, pp. 12–15. (in Russ.).
Tsaruk A.V., Khushbaktova Z.A., Syrov V.N., Zakhidova L.T. Patologiya, 2007, no. 4, pp. 21–22. (in Russ.).
Tsaruk A.V., Zakhidova L.T., Khushbaktova Z.A., Syrov V.N., Isayev M.I. Doklady AN RUz, 2011, no. 2, pp. 59–62. (in Russ.).
Tsaruk A.V., Isaev I.M., Agzamova M.A., Vypova N.L., Khushbaktova Z.A., Syrov V.N., Isaev M.I. Uzbek Biologi-cal Journal. 2010. N5. Pp. 10–12. DOI: 10.1007/s11094-010-0388-7.
Agzamova M.A., Duschanova G.M., Rakhmatov Kh.A., Janibekov A.A. American J. Planta Sciences, 2020, vol. 11, no. 4, pp. 569–577. DOI: 10.4236/ajps.2020.114042.
![](http://journal.asu.ru/public/journals/1/article_8314_cover_ru_RU.png)
Copyright (c) 2021 chemistry of plant raw material
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.