INVESTIGATION OF THE BIOLOGICAL ACTIVITY OF SEA BUCKTHORN MEAL FLAVONOIDS USING SPECIFIC BIOTEST SYSTEMS
UDC 579:634.74
Abstract
The need to expand the raw material base for obtaining flavonoids is due to the wide spectrum of their biological activity. The purpose of this work is to study the biological activity of a complex of bioflavonoids, quercetin and rutin on specific enzyme biotest systems in vitro. The objects of the study were: a complex of bioflavonoids from fat-free sea buckthorn meal Hippophae rhamnoides L. and individual flavonoids rutin and quercetin isolated from it. The study was carried out by methods of detecting the biological activity of substances using specific enzyme biotest systems in vitro. It was revealed that rutin and a complex of bioflavonoids have antioxidant properties – the rate of glutathione reductase reaction increased by 64 and 51% of control, respectively, and catalase – by 15%. Quercetin exhibits antimicrobial activity and also reduces the rate of the enzymatic iNOS reaction by 24% of the control, which indicates the anti-inflammatory properties of this sample. Rutin and a complex of bioflavonoids from sea buckthorn meal increased the iNOS reaction rate by 14 and 28%, respectively, which indicates the immunostimulating properties of these samples. In the course of a microbiological study, it was found that all samples have weak bacteriostatic activity against gram-positive and gram-negative bacteria Staphylococcus aureus ATCC 6538 (209-P) and Pseudomonas aeruginosa ATCC 9027. Fungistatic activity was confirmed against the yeast-like fungi Candida albicans ATCC 10231 of quercetin and the complex. The results obtained make it possible to consider a complex of bioflavonoids, quercetin and rutin as promising active substances in antioxidant, antimicrobial, fungistatic and anti-inflammatory drugs.
Downloads
Metrics
References
Makarenko O.A., Levitskiy A.P. Fiziologiya i biokhimiya kul'turnykh rasteniy, 2013, Vol. 45, no. 2, pp. 100–112. (in Russ.).
Manach C., Morand G., Scalbert A., Remesy C. The American Journal of Clinical Nutrition, 2005, no. 8, pp. 230–231.
Novza YU.A., Popova E.M. Problemy ekolohichnoyi biotekhnolohiyi, 2016, no. 1, pp. 24–33. (in Russ.).
Kurkin V.A., Kurkina A.V., Avdeyeva Ye.V. Fundamental'nyye issledovaniya, 2013, no. 11-9, pp. 1897–1901. (in Russ.).
Hensel A., Bauer R., Heinrich M. et al. Planta Medica, 2020, vol. 86, no. 10, pp. 659–664. DOI: 10.1055/a-1177-4396.
Mhatre S, Srivastava T, Naik S, Patravale V. Phytomedicine, 2020, Article 153286. DOI: 10.1016/j.phymed.2020.153286. (in Press).
Sundararajan A., Ganapathy R., Huan L. et al. Antiviral Res., 2010, vol. 88, no. 1, pp. 1–9. DOI: 10.1016/j.antiviral.2010.06.014.
Shkol'nikova M.N., Aver'yanova Ye.V., Rozhnov Ye.D., Batashov Ye.S. Industriya pitaniya, 2020, vol. 5, no. 3, pp. 61–69. DOI: 10.29141/2500-1922-2020-5-3-7. (in Russ.).
Wu Y., Wang B., Wang Y. Zhongguo Yaolixue tongbao. Chinese pharmacological bulletin, 1997, no. 13(1), pp. 53–58.
Li С., Wang T., Zhang C. et al. Gene, 2016, vol. 577, no. 2. Pp. 275–280. DOI: 10.1016/j.gene.2015.12.012.
Shvedova M.V., Anfinogenova YA.D., Popov S.V. Sibirskiy meditsinskiy zhurnal, 2016, vol. 31, no. 3, pp. 7–15. DOI: 10.29001/2073-8552-2016-31-3-7-15. (in Russ.).
Vatutin N.T., Kalinkina N.V., Keting Ye.V. Arkhiv klinicheskoy i eksperimental'noy meditsiny, 2001, no. 3, pp. 287–290. (in Russ.).
Tarakhovskiy YU.S., Kim YU.A., Abdrasilov B.S., Muzafarov Ye.N. Flavonoidy: biokhimiya, biofizika, meditsina. [Flavonoids: biochemistry, biophysics, medicine]. Moscow, 2013, 308 p. (in Russ.).
Patent 2711728 (RU). 2020. (in Russ.).
Rozhnov Ye.D., Aver'yanova Ye.V., Shkol'nikova M.N., Selivanov N.I. Vestnik KrasGAU, 2020, vol. 162, no. 9, pp. 177–184. DOI: 10.36718/1819-4036-2020-9-177-184. (in Russ.).
Patent 2181892 (RU). 2002. (in Russ.).
Patent 2181891 (RU). 2002. (in Russ.).
Strelkova L.B., Kondakova N.V., Dubinskaya V.A. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2013, no. 11, pp. 75–80. (in Russ.).
Lupanova I.A., Mizina P.G., Martynchik I.A., Rogozhnikova Ye.P. Biofarmatsevticheskiy zhurnal, 2020, vol. 12, no. 4, pp. 3–7. DOI: 10.30906/2073-8099-2020-12-4-45-49. (in Russ.).
Adamov G.V., Mel'nikov Ye.S., Lupanova I.A., Radimich A.I., Saybel O.L. Razrabotka i registratsiya lekarstvennykh sredstv, 2020, vol. 9, no. 3, pp. 143–149. DOI: 10.33380/2305-2066-2020-9-3-143-149. (in Russ.).
Lupanova I.A., Mizina P.G., Sidelnikov N.I., Gulenkov A.S. Periodico Tche Quimica, 2019, vol. 16, issue 31, pp. 484–490.
Rukovodstvo po provedeniyu doklinicheskikh issledovaniy lekarstvennykh sredstv. [Guidelines for conducting preclinical studies of drugs.]. Part 1. Ed. A.N. Mironov. Moscow, 2012, 944 p. (in Russ.).
Beutler E. Red cell metabolism. Edinburgh, 1986. 126 p.
![](http://journal.asu.ru/public/journals/1/article_8859_cover_ru_RU.png)
Copyright (c) 2020 chemistry of plant raw material
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.