METHOD OF QUANTITATIVE DETERMINATION OF THE SUM OF TANNINS IN EUTERPE OLERACEA FRUITS
UDC 615.322:543.422.3
Abstract
Fruits of Euterpe oleracea are widely used in foreign medical practice as an antioxidant. The fruits of Euterpe contain tannins. The most common method of quantitative determination of tannins is spectrophotometry. The purpose of this work is to determine the content of the sum of tannins in the fruits of Euterpe by spectrophotometry
Quantitative determination of the amount of tannins in the fruits of Euterpe by direct spectrophotometry was carried out. To confirm the presence of tannins in the fruits of Euterpe, qualitative reactions were used (1% solution of iron-ammonium alum, 1% solution of vanillin in concentrated hydrochloric acid). The presence of tannins was confirmed by direct spectrophotometry in extracts from euterpe fruits, the analytical maxima of the studied compounds were determined at about 282±2 nm, which corresponds to the maximum absorption of catechin. The optimal conditions for the extraction of tannins from the raw materials of this plant (extractant – ethyl alcohol 40%; the ratio of "raw material – extractant" – 1 : 100; extraction time – 60 minutes; the degree of grinding of raw materials – 1.0 mm) are justified. It was determined that the average error in determining the content of tannins in the fruits of euterpe with a confidence probability of 95% is ±1.59%. It was revealed that the content of tannins in the fruits of euterpe is 8.90%.
Downloads
Metrics
References
De Moura R.S., Resende А.C. J. Cardiovasc Pharmacol., 2016, vol. 68, pp. 19–26.
De Souza M.O., Souza e Silva L., de Brito Magalhаes C.L., de Figueiredo B.B., Costa D.C., Silva M.E. Nutrition Re-search, 2012, vol. 32, pp. 976–984.
Udani J.K., Singh B.B., Singh V.J., Barrett M.L. Nutr J., 2011, vol. 2, no. 10, p. 45.
Heinrich M., Dhanji T., Casselman I. Phytochem. Lett., 2011, vol. 4, pp. 10–21.
Carvalho A.V., Ferreira da Silveira T., Mattietto R.d.A., Padilha de Oliveira M.d.S., Godoy H.T. Journal of the Science of Food and Agriculture, 2017, vol. 97, pp. 1467–1474.
Samylina I.A., Balandina I.A. Farmatsiya, 2004, no. 2, pp. 39–41. (in Russ.).
Korenskaya I.M., Ivanovskaya N.P., Izmalkova I.Ye., Mal'tseva A.A., Karakozova S.A. Fitokhimicheskiy analiz lekarstvennogo rastitel'nogo syr'ya. [Phytochemical analysis of medicinal plant materials]. Voronezh, 2012, pp. 77–76. (in Russ.).
Semenyuta K.N., Kurkin V.A., Shmygareva A.A., San'kov A.N. Vestnik Voronezhskogo gosudarstvennogo universi-teta. Seriya: Khimiya. Biologiya. Farmatsiya, 2020, no. 2, pp. 73–77. (in Russ.).
Mal'tseva A.A., Chistyakova A.S., Sorokina A.A. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: Khimiya. Biologiya. Farmatsiya, 2013, no. 2, pp. 203–205. (in Russ.).
Del Pozo-Insfran D., Brenes C.H., Talcott S.T. J. Agric. Food Chem., 2004, vol. 52, no. 6, pp. 1539–1545.
Del Pozo-Insfran D., Percival S.S., Talcott S.T. Journal of Agricultural and Food Chemistry, 2006, vol. 54, pp. 1222–1229.
Garzon G.A., Narvaez-Cuenca C.E., Vincken J.P., Gruppen H. Food Chemistry, 2017, vol. 217, pp. 364–372.
Bataglion G.A., da Silva F.M.A., Eberlin M.N., Koolen H.H.F. Food Chemistry, 2015, vol. 180, pp. 280–287.
Ladygina Ye.Ya., Safronich L.N., Otryashenkova V.E. i dr. Khimicheskiy analiz lekarstvennykh rasteniy. [Chemical analysis of medicinal plants]. Moscow, 1983, 176 p. (in Russ.).
Copyright (c) 2021 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
