DETERMINATION OF BIOLOGICALLY ACTIVE COMPOUNDS IN LIQUID EXTRACT OF PLANTS POLYGONUM HIDROPIPER L., BURSA PASTORIS, CALENDULAE OFFICINALIS AND URTICA DIOICA L.
UDC 615.07; 543.544
Abstract
Methods have been developed for the quantitative determination of biologically active compounds - flavonoids and vitamin K1 in the liquid extract "Extradent" obtained from the aerial part of the knotweed (Polygonum hidropiper L.), shepherd's purse (Bursa pastoris), calendula flowers (Calendulae officinalis) and nettle leaves (Urtica dioica). L.), by high performance liquid chromatography (HPLC). As a result, components corresponding to the retention time of standard samples of robinin, rutin, and quercetin were found on the chromatogram of the extract. It was revealed that the content of robinin was 0.37%, rutin 0.014%, quercetin 0.00045%, and vitamin K1 0.005%. The same method of mass spectrometry with inductively coupled plasma (ICP-MS) was used to determine the quantitative content of macro- and microelements of the liquid extract "Extradent". The highest content of macroelements is in the proportion of potassium (0.65%), phosphorus (0.2%), and among the microelements of iron is 15.716 mg/l, selenium – 0.046 mg/l. The content of heavy metal salts in the liquid extract is within the normal range and totals 1.155 mg/l. Of these, the main content is zinc and copper.
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Vedenkina Yu.I. Razrabotka i standartizatsiya lekarstvennogo sredstva rastitel'nogo proiskhozhdeniya, reko-menduyemogo pri zabolevaniyakh parodonta: avtoref. diss. … kand. farm. nauk. [Development and standardization of a herbal medicine recommended for periodontal diseases: abstract of thesis. diss. ...cand. pharm. Sci.]. Moscow, 2009, pp. 3–4. (in Russ.).
Georgiyevskiy V.P. Tekhnologiya i standartizatsiya lekarstv. Sbornik nauchnykh trudov. [Technology and standardiza-tion of drugs. Collection of scientific papers]. Khar'kov, 1996, vol. 1, 784 p. (in Russ.).
Georgiyevskiy V.P. Tekhnologiya i standartizatsiya lekarstv. Sbornik nauchnykh trudov. [Technology and standardiza-tion of drugs. Collection of scientific papers]. Khar'kov, 2000, vol. 2, 781 p. (in Russ.).
Makarevich I.F. Tekhnologiya i standartizatsiya lekarstv. [Technology and standardization of drugs]. Khar'kov, 1996, vol. 1, pp. 5–27. (in Russ.).
Kutikhina Ye.A., Kurdyukov Ye.Ye., Moiseyeva I.Ya., Bibyakova L.N., Finayonova N.V. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Yestestvennyye nauki, 2021, no. 1, pp. 16–23. DOI: 10.21685/2307-9150-2021-1-2. (in Russ.).
Georgiyevskiy V.P. Tekhnologiya i standartizatsiya lekarstv. [Technology and standardization of drugs]. Khar'kov, 2000, vol. 2, pp. 34–45. (in Russ.).
Litvinenko V.I., Georgiyevskiy V.P., Ammosov A.S., Popova T.V., Fursa N.S. Solodka. Sistematika, khimiya, tekhnologiya, standartizatsiya, farmakologiya, klinika. [Licorice. Systematics, chemistry, technology, standardization, pharmacology, clinic]. Yaroslavl', 2014, 466 p. (in Russ.).
Georgiyevskiy V.P. Terpenoidy: dostizheniya i perspektivy primeneniya v oblasti khimii, tekhnologii proizvodstva i meditsiny. [Terpenoids: achievements and prospects for application in the field of chemistry, production technology and medicine]. Karaganda, 2008, pp. 16–34. (in Russ.).
Kurkina A.V. Fundamental'nyye issledovaniya, 2013, no. 10, pp. 606–609. (in Russ.).
Zharkeyeva A.M., Shevchenko A.S., Korul'kin D.Yu., Muzychkina R.A. Vestnik KazNMU, 2018, no. 3, pp. 241–245. (in Russ.).
Peng Zh., Strack D., Baumert A., Subramaniam R., Khang Goh N., Chia T., Tan S., Chia L.S. Phytochemistry, 2003, vol. 62 (2), pp. 219–228. DOI: 10.1016/s0031-9422(02)00504-6.
Frum A. Acta Universitatis Cibiniensis Series E: Food technology, 2017, vol. 2, pp. 97–101. DOI: 10.1515/aucft-2017-0020.
Hernandez-Saavedra D., Perez-Ramirez I.F., Ramos-Gomez M., Medoza-Diaz S., Loarca-Pina G., Reynoso-Camacho R. Med. Chem. Res., 2016, vol. 25, pp. 163–172. DOI: 10.1007/s00044-015-1454-1.
Sausserde R., Kampuss K. Harmonious Agriculture, 2014, pp. 161–165.
Peng J., Hu T., Li J., Du J., Zhu K., Cheng B., Li K. Oxid. Med. Cell. Longev., 2019, vol. 2019, pp. 1–14. DOI: 10.1155/2019/7202695.
Dar M.A., Mir H.M., Mohi-ud-din R., Mir P.A., Masoodi M.H., Akbar S., Mir Sh. A., Sawhney G. Current Tradi-tional Medicine, 2021, vol. 7, pp. 168–179. DOI: 10.2174/2215083806666200203142107.
Nencu I., Vlase L., Istudor V., Duţu L.E., Gîrd C.E. Farmacia, 2012, vol. 60, pp. 493–500.
Repajić M., Cegledi E., Zorić Z., Pedisić S, Elez Garofulić I., Radman S., Palčić I., Dragović-Uzelac V. Foods, 2021, vol. 10, pp. 2–18. DOI: 10.3390/foods10010190.
Yunuskhodzhayeva H.A., Poyonov M.M., Uzokboyev Sh.N. Farmatsevticheskiy vestnik Uzbekistana, 2020, no. 1, pp. 58–62. (in Russ.).
Abdullabekova V.N., Eshbakova K.A., Yunusxodjayeva N.A. 7th International Symposium on the Chemistry of Natu-ral Compounds. Tashkent, 2007, p. 353.
Abdullabekova V.N., Muhamatkhanova R.F., Shamyanov I.D., Yunusxodjayeva N.A. 7th International Symposium on the Chemistry of Natural Compounds. Tashkent, 2007, p. 355.
Djuraeva A.A., Abdullabekova V.N. Indian Journal of Forensic Medicine and Toxicology, 2020, vol. 14, pp. 7609–7617. DOI: 10.37506/ijfmt.v14i4.12852.
Kurkin V.A. Farmakognoziya: uchebnik dlya studentov farmatsevticheskikh vuzov (fakul'tetov.). [Pharmacognosy: a textbook for students of pharmaceutical universities (faculties)]. Samara, 2007, 1239 p. (in Russ.).
Kurkina A.V. Flavonoidy farmakopeynykh rasteniy. [Flavonoids of pharmacopoeial plants]. Samara, 2012, 262 p. (in Russ.).
Smolarz H.D. Drug Research, 2002, vol. 59, pp. 145–148.
Dau N.V., Thang V.N. Journal of Chemistry, 2004, vol. 42, pp. 512–515.
Dzoyem J.P., Nkuete A.H.L., Kuete V., Tala M.F., Wabo H.K., Guru S.K., Rajput V.S., Sharma A., Tane P., Khan I.A., Saxena A.K., Laatsch H., Tan N.H. Planta Medica, 2012, vol. 78, pp. 787–792.
Yunuskhodzhayeva H.A., Abdullabekova V.N, Bekchanov Kh.N. Farmatsevticheskiy zhurnal, 2006, no. 4, pp. 37–40. (in Russ.).
Vial J., Jardy A. Encyclopedia of Chromatography. Taylor & Francis, 2010, vol. 3, pp. 1975–1976.
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