IDENTIFICATION OF TERPENE LACTONES AND FLAVONOL GLYCOSIDES IN PREPARATIONS BASED ON GINKGO BILOBA EXTRACT AND A NEW WAY OF SEMI QUANTITATIVE DETERMINATION OF FLAVONOL GLYCOSIDES BY 1H NMR SPECTROSCOPY
Abstract
The composition of terpen lactones and flavonol glycosides of commercial preparation series based on ginkgo biloba extracts was explored by quantitative 1H NMR spectroscopy. The content of individual terpen lactones was determined using DMSO-d6 and acetone-d6 solvents. The structure effect of flavonol glycosides on the signal of the hydroxyl proton at position 5 of ring A was examined. A new approach of semi quantitative determination of flavonol glycosides amount by this signal, which is superposition of the singlets of individual flavonoids on the area from 12,5 to 12,65 ppm in DMSO-d6 solvent, was offered. The offered method can be used for detection of counterfeit ginkgo biloba extracts by cheap individual aglicones or rutin, because the signals of individual aglicones have different from flavonol glycosides chemical shifts.
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