INVESTIGATION OF THE GROWTH-STIMULATING ACTIVITY OF AQUEOUS EXTRACTS OF CONIFEROUS WOODY GREENS
UDC 502 663.86
Abstract
The paper considers the possibility of using water extracts of conifers to increase the germination of seeds of Siberian spruce (Picea obovata Ledeb) and Scots pine (Pepys sylvestris L.) for long-term storage. It was found out that in the woody greenery of conifers there are substances with provitamin and growth-stimulating activity, namely carotenoids (0.014 g/l) and phenolic compounds (2.61 g/l). As raw materials, pine and spruce tree greens were taken, selected in the area of the Krasnoyarsk Pillars National Park. Extracts were prepared from coniferous tree greens as follows: dried and crushed pine and spruce tree greens were extracted with tert-butylmethyl ether. The extract was treated with an aqueous solution of an alkaline agent, then an aqueous-alkaline solution was separated. Distilled water was taken as a control. For the experiment, seeds of scots pine and Siberian spruce harvested from 2017 to 2021 were used. The seeds were germinated in Petri dishes, filter paper was used as a litter. The seedlings were accounted for according to the current GOST on day 7, 10, respectively. Germination was determined on day 15 for pine seeds and on day 20 for spruce seeds. Per 100 pcs. the following concentrations of the extract of an aqueous solution were taken from the seeds, ml of extract per 1 liter: 0.25 : 1; 0.5 : 1; 0.10 : 1; 0.05 : 1; 0.025 : 1. It was found out that with an increase in the shelf life, the germination of seeds decreases, and the greatest germination of seeds is observed when using an extract with a concentration of extractive substances (0.05 : 1) and seeds of 2020 and 2021 collection years.
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