THE METHODOLOGY IMPROVING FOR QUANTITATIVE DETERMINATION OF THE FLAVONOIDS AMOUNT IN THE COMMON MYRTLE LEAVES (MYRTUS COMMUNIS L.)
UDC 615.322: 547.972+543.544
Abstract
Common myrtle (Myrtus communis L.) is a promising type of vegetable raw material, which is actively used abroad for the manufacture of drugs with antiseptic, antimicrobial, disinfecting, stabilizing effect. In our opinion, flavonoids contained in common myrtle leaves contribute to antimicrobial and antioxidant activity, along with myrtocommulones. This article discusses the results of a flavonoid composition study of the common myrtle leaves (Myrtus communis L.). As a result of column chromatography on silica gel KSK 50/100, the dominant flavonoid myricitrin, was isolated. Based on the data of UV, 1H-NMR, 13C-NMR spectroscopy, mass spectrometry, as well as the results of acid hydrolysis, myricitrin was identified as 3-O-α-L-rhamnopyranoside of myricetin.
It was determined that in all UV spectra of extracts from common myrtle leaves, a bathochromic shift of the long-wavelength band is observed in the presence of a 3% alcohol solution of AlCl3, which confirms the presence of a dominant flavonoid. Under the conditions of differential spectrophotometry of myricitrin, the maximum absorption is observed in the 418 nm region. As a result of the conducted research, the method of quantitative determination of the flavonoids amount in the common myrtle leaves has been improved. It was determined that the content of the flavonoids sum in terms of myricitrin in all studied samples varies from 1.57±0.06 to 1.99±0.11%.
Downloads
Metrics
References
Nikolayevskiy V.V. Aromaterapiya: spravochnik. [Aromatherapy: handbook]. Moscow, 2000, p. 52. (in Russ.).
Logvinenko L.A. Agrarnyy vestnik Urala, 2017, no. 9 (163), pp. 15–21. https://doi.org/10.32417/article_5d47f7f2cc7a67.55851754. (in Russ.).
Gosudarstvennaya Farmakopeya Rossiyskoy Federatsii XV izd. [State Pharmacopoeia of the Russian Federation XV edition]. Moscow, 2023. URL: https://pharmacopoeia.regmed.ru/pharmacopoeia/izdanie-15/. (in Russ.).
European Pharmacopoeia (Ph. Eur.) 11th edition. 2023. URL: https://www.edqm.eu/en/european-pharmacopoeia-ph.-eur.-11th-edition.
Pharmacopee Francaise XI edition, Préparations homéopathiques. 2017, pp. 2012–2015.
Sisay M., Gashaw T. J. Evid. Based Complement Altern. Med., 2017, vol. 22(4), pр. 1035–1043. https://doi.org/10.1177/2156587217718958.
Migliore J., Baumel A., Juin M., Médail F. Journal of Biogeography, 2012, vol. 39, pр. 942–956. https://doi.org/10.1111/j.1365-2699.2011.02646.x.
Flamin G., Cioni P.L., Morelli I., Maccioni S., Baldini R. Food Chemistry, 2004, vol. 85, no. 4, pр. 599–604. https://doi.org/10.1016/j.foodchem.2003.08.005.
Bakova Ye.Yu., Plugatar' Yu.V., Bakova N.N., Konovalov D.A. Khimiya rastitel'nogo syr'ya, 2019, no. 3, pp. 217–223. https://doi.org/10.14258/jcprm.2019034917. (in Russ.).
Nicoletti R., Ferranti P., Caira S., Misso G., Castellano M., Di Lorenzo G., Caraglia M. World Journal Microbiol. Bio-technol., 2014, vol. 30, pp. 1047–1052. https://doi.org/10.1007/s11274-013-1523-x.
Gerbeth K., Hüsch J., Meins J., Rossi A., Sautebin L., Wiechmann K., Werz O., Skarke C., Barrett J.S., Schubert-Zsilavecz M., Abdel-Tawab M. Planta Medica, 2012, vol. 78, no. 18, pp. 1932–1938. https://doi.org/10.1055/s-0032-1327881.
Medda S., Fadda A., Dessena L., Mulas M. Agronomy, 2021, vol. 11, no. 6, 1059. https://doi.org/10.3390/agronomy11061059.
Yoshimura M., Amakura Y., Tokuhara M., Yoshida T. Journal of Natural Medicines, 2008, vol. 62, no. 3, pp. 366–368. https://doi.org/10.1007/s11418-008-0251-2.
Alipour G., Dashti S., Hosseinzadeh H. Phototherapy Research, 2014, vol. 28, no. 8, pp. 1125–1136. https://doi.org/10.1002/ptr.5122.
Hennia A., Miguel M.G., Nemmiche S. Medicines, 2018, vol. 5, no. 3, 89. https://doi.org/10.3390/medicines5030089.
Mansour R.B., Megdiche-Ksouri W., Nefzi N., Soumaya B., Ksouri R., Giroux-Metgès M.A., Talarmin H. Austin Journal of Nutrition & Metabolism, 2022, vol. 9, no. 1, pp. 1–9. https://doi.org/10.26420/austinjnutrmetab.2022.1121.
Kurkin V.A., Khusainova A.I., Kurkina A.V., Bakova N.N., Bakova Ye.Yu. Khimiya rastitel'nogo syr'ya, 2021, no. 1, pp. 159–166. https://doi.org/10.14258/jcprm.2021017423. (in Russ.).
Bakova N.N., Bakova E.Y., Paliy A.E., Konovalov D.A. Acta Horticulturae, 2021, vol. 1324, pp. 361–365. https://doi.org/10.17660/ActaHortic.2021.1324.56.
Belodubrovskaya G.A. i dr. Entsiklopedicheskiy slovar' lekarstvennykh rasteniy i produktov zhivotnogo pro-iskhozhdeniya: Ucheb. Posobiye. [Encyclopedic Dictionary of Medicinal Plants and Products of Animal Origin: Text-book. Manual]. St. Petersburg, 1999, p. 196. (in Russ.).
Shishkin B.K., Bobrov Ye.G. Flora SSSR. [Flora of the USSR]. Moscow, 1949, vol. 15, pp. 554–555. (in Russ.).
Kiselova T.L., Smirnova Yu.A. Lekarstvennyye rasteniya v mirovoy meditsinskoy praktike: gosudarstvennoye regulirovaniye nomenklatury i kachestva. [Medicinal plants in world medical practice: state regulation of nomenclature and quality]. Moscow, 2009, 295 p. (in Russ.).
Gerbarii Nikitskogo botanicheskogo sada [Herbariums of the Nikitsky Botanical Garden]. URL: https://nikitasad.ru/science/gerbarij-nikitskogo-botanicheskogo-sada/. (in Russ.).
OFS.1.2.1.1.0003.15. Spektrofotometriya v UF i vidimoy oblastyakh [OFS.1.2.1.1.0003.15. Spectrophotometry in the UV and visible regions]. (in Russ.).
Kurkina A.V. Izvestiya Samarskogo nauchnogo tsentra RAN, 2012, no. 1-9, pp. 2253–2256. (in Russ.).
Kurkin V.A., Avdeeva E.V., Kurkina A.V. et al. Drug Discovery and Therapy World Congress. Boston; USA, 2013, p. 170.
OFS 1.1.0013.15. Statisticheskaya obrabotka rezul'tatov khimicheskogo eksperimenta [OFS 1.1.0013.15. Statistical processing of chemical experiment results]. (in Russ.).
Copyright (c) 2025 Chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
