METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN THE RAW MATERI-ALS OF THE BETONICA OFFICINALIS
UDC 615.322:543.422.3
Abstract
The dried herb of the Betonica officinalis L., (Lamiaceae). The raw materials of the B. officinalis grown in the Penza region were studied. The purpose of this work is to determine the amount of flavonoids in the herb of the B. officinalis by spectrophotometry.
The quantitative determination of the amount of flavonoids in the herb of the letter by differential spectrophotometry was carried out. The presence of flavonoids was confirmed by differential spectrophotometry in extracts from the herb B. officinalis, and the analytical maxima of the studied compounds – 395 nm – were determined. Quantitative determination of the amount of flavonoids in the herb of the letter by differential spectrophotometry was carried out in terms of luteolin-7-glucoside. In order to recalculate the content of substances of a phenolic nature in the extraction of letter letters from grass to luteolin-7-glucoside, we used the specific absorption index of luteolin-7-glucoside at λ=395 nm for differential spectrophotometry. The optimal conditions for the extraction of flavonoids from the raw materials of this plant are justified (extractant – ethyl alcohol 70%; the ratio of "raw material – extractant" is 1 : 200; extraction time is 90 minutes; the degree of grinding of raw materials is 1.0 mm). It was determined that the average error in determining the content of flavonoids in the herb of the B. officinalis with a confidence probability of 95% is ±3.66%. It was revealed that the content of flavonoids in the herb of the B. officinalis is 0.74%.
Downloads
Metrics
References
Saparklycheva S.Ye., Chulkova V.V. Vestnik biotekhnologii, 2020, no. 1(22), p. 14. (in Russ.).
Logvinenko L.A. Rasteniya Kryma: Prelestnyye sosedi. [Plants of Crimea: Charming Neighbors]. Simferopol', 2016, pp. 207–208. (in Russ.).
Savchenko O.M., Fedorova Ye.A. Vestnik KrasGAU, 2022, no. 4(181), pp. 17–23. https://doi.org/10.36718/1819-4036-2022-4-17-23. (in Russ.).
Gosudarstvennaya farmakopeya Rossiyskoy Federatsii. 14 izd. [State Pharmacopoeia of the Russian Federation. 14th edition]. Moscow, 2018. URL: http://femb.ru/femb/pharmacopea.php. (in Russ.).
Kurdyukov Ye.Ye., Pleshakova D.A., Glebova N.N. i dr. Mezhdunarodnyy nauchno-issledovatel'skiy zhurnal, 2023, no. 11(137). https://doi.org/10.23670/IRJ.2023.137.143. (in Russ.).
Polukhina T.S., Inizarova D.R., Pogudina S.A. Nauka i obrazovaniye: sokhranyaya proshloye, sozdayom budush-cheye: sbornik statey XII Mezhdunarodnoy nauchno-prakticheskoy konferentsii. [Science and education: preserving the past, creating the future: collection of articles of the XII International scientific and practical conference]. Penza, 2017, vol. 1, pp. 170–172. (in Russ.).
Kurdyukov E.E., Vodop'yanova O.A., Moiseeva I.Y., Semenova E.F. Moscow University Chemistry Bulletin, 2021, vol. 76, no. 3, pp. 224–226. https://doi.org/10.3103/S0027131421030044.
Polukhina T.S., Inizarova D.R., Pogudina S.A. Nauka i innovatsii v XXI Veke: Aktual'nyye voprosy, otkrytiya i dosti-zheniya: sbornik statey V Mezhdunarodnoy nauchno-prakticheskoy konferentsii. [Science and Innovations in the 21st Century: Current Issues, Discoveries and Achievements: collection of Articles from the V International Scientific and Practical Conference]. Penza, 2017, vol. 1, pp. 213–215. (in Russ.).
Gavrilova N.A., Shurygina M.S., Kurdyukov Ye.Ye. i dr. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Yestestvennyye nauki, 2020, no. 2(30), pp. 5–13. https://doi.org/10.21685/2307-9150-2020-2-1. (in Russ.).
Ladygina Ye.Ya., Safronich L.N., Otryashenkova V.E. i dr. Khimicheskiy analiz lekarstvennykh rasteniy. [Chemical analysis of medicinal plants]. Moscow, 1983, 176 p. (in Russ.).
Kurkina A.V. Flavonoidy farmakopeynykh rasteniy: monografiya. [Flavonoids of pharmacopoeial plants: monograph]. Samara, 2012, 290 p. (in Russ.).
Ivanov V.V., Denisenko O.N. Fundamental'nyye issledovaniya, 2013, no. 10 (2), pp. 374–377. (in Russ.).
Samylina I.A. Farmatsiya, 2004, no. 2, pp. 39–41. (in Russ.).
Andreyeva V.Yu., Kalinkina G.I., Li V.V. Khimiya rastitel'nogo syr'ya, 2019, no. 3, pp. 161–168. https://doi.org/10.14258/jcprm.2019034683. (in Russ.).
Kurkin V.A., Pravdivtseva O.Ye., Morozova T.V., Kurkina A.V., Shaykhutdinov I.Kh., Kretova A.A. Khimiya ras-titel'nogo syr'ya, 2019, no. 3, pp. 137–144. https://doi.org/10.14258/jcprm.2019035201. (in Russ.).
Georgiyevskiy V.P., Komissarenko N.F., Dmitruk S.Ye. Biologicheski aktivnyye veshchestva lekarstvennykh ras-teniy. [Biologically active substances of medicinal plants]. Novosibirsk, 1990, pp. 236–238. (in Russ.).
Korul'kin D.Yu., Abilov Zh.A., Muzychkina R.A., Tolstikov G.A. Prirodnyye flavonoidy. [Natural flavonoids]. Novo-sibirsk, 2007, 232 p. (in Russ.).
Tarakhovskiy Yu.S., Kim Yu.A., Abdrasilova B.S., Muzafarova Ye.N. Flavonoidy: biokhimiya, biofizika, meditsina. [Flavonoids: biochemistry, biophysics, medicine]. Pushchino, 2013, 310 p. (in Russ.).
Kurkin V.A., Braslavskiy V.B., Avdeyeva Ye.V., Pravdivtseva O.Ye. i dr. Proizvodstvennaya praktika po standartizatsii lekarstvennogo rastitel'nogo syr'ya i fitopreparatov: ucheb. posobiye dlya studentov farmatsevticheskikh vuzov. [Industrial practice in standardization of medicinal plant raw materials and phytopreparations: a teaching aid for students of pharmaceutical universities]. Samara, 2007, 126 p. (in Russ.).
Kurkina A.V. Eksperimental'no-teoreticheskoye obosnovaniye podkhodov k standartizatsii syr'ya i preparatov farmakopeynykh rasteniy, soderzhashchikh flavonoidy: avtoref. … dokt. farm. nauk. [Experimental and theoretical sub-stantiation of approaches to standardization of raw materials and preparations of pharmacopoeial plants containing fla-vonoids: author's abstract. ... doctor of pharm. sciences]. Samara, 2013, 48 p. (in Russ.).
Copyright (c) 2025 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
