QUANTITATIVE DETERMINATION OF COPTISINE IN THE HERB OF CORYDALIS BRACTEATA THROUGH HPLC ANALYSIS
UDC 615.322
Abstract
Coptisine belongs to the group of protoberberine alkaloids of the isoquinoline series and is found in large quantities in Corydalis bracteata aerial parts. According to the literature data, coptisine possesses antibacterial, anti-inflammatory, cardioprotective and antitumor effects, thus, a wide range of pharmacological activity necessitates the determination of its quantitative content in plant raw materials. Previously, the authors of the article developed a method for isolating coptisine from the aerial parts of C. bracteata, the aim of this study is to develop a method for the quantitative determination of coptisine in C. bracteate and the selection of optimal extraction conditions. As a result of the quantitative determination of coptisine in the C. bracteata aerial part, it was found, that, the maximum yield of coptisine from raw materials, when extracted under optimal conditions, is 2.59%, while the maximum content of coptisine is contained in the plants leaves and is 3.97%. As a result of the research, validation of the quantitative analysis of coptisine using HPLC was carried out. There is no information in the literature about the method of quantitative determination of coptisine, which makes this study novel.
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