COMPONENT COMPOSITION, ANTIRADICAL AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL OF LEMON BALM LEAVES

UDC 615.322+547.913+543.544

  • Aleksandr Alekseevich Efremov Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Institute of Space Technologies, FRC KSC SB RAS Email: aefremov15@mail.ru
  • Elena Evgenievna Saveleva Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University Email: saveleva_ee@mail.ru
  • Nadezhda Anatolyevna Bulgakova Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University Email: bulgakovana@bk.ru
  • Tatyana Vladimirovna Rukosueva Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University Email: ru-ta@inbox.ru
  • Veronika Artemovna Volkova Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University Email: verunya.volkova.02@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20250115329
Keywords: Melissa officinalis L., essential oil fractions, antiradical activity, antimicrobial activity

Abstract

The essential oil and its separate fractions were isolated from the lemon balm leaves collected before the plant budding using the method of exhaustive hydrosteam distillation. The component composition of the oil and fractions was determined using the method of chromatography-mass spectrometry. The essential oil mainly contains sesquiterpene compounds and does not contain such monoterpenoids as citral, citronellal, geraniol. The antiradical activity of the oil and fractions was assessed during the reaction with a stable free 2,2-diphenyl-1-picrylhydrazyl radical. The values of the antiradical activity of the whole oil and its separate fractions correlate with the content of oxygen-containing terpenoids. The correlation coefficient is 0.81. The antimicrobial activity was studied using the disk diffusion method against strains of Staphylococcus aureus (MSSA), Staphylococcus aureus (MRSA), Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Whole essential oil of lemon balm leaves and its separate fractions completely inhibit the growth of Staphylococcus aureus (MSSA). Fraction 1, collected during the first hour, showed fungicidal activity against Candida albicans, completely inhibiting the growth of the strain, both undiluted and diluted in a 1 : 1 ratio with 96% ethyl alcohol. Essential oil of lemon balm leaves collected before the plant budding is promising for further studies of its biological activity.

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Author Biographies

Aleksandr Alekseevich Efremov , Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Institute of Space Technologies, FRC KSC SB RAS

Doctor of Chemical Sciences, Professor, Researcher of the Laboratory of Fundamental and Personalized Pharmacy, Head of the Department of Complex Processing of Plant Raw Materials

Elena Evgenievna Saveleva , Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Candidate of Pharmaceutical Sciences, Associate Professor, Head of the Pharmacy Department with a PO course

Nadezhda Anatolyevna Bulgakova , Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Candidate of Chemical Sciences, Associate Professor, Associate Professor of the Pharmacy Department with a PO course

Tatyana Vladimirovna Rukosueva , Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Candidate of Biological Sciences, Associate Professor of the Microbiology Department named after Associate Professor B.M. Zelmanovich

Veronika Artemovna Volkova , Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

student

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Published
2025-03-07
How to Cite
1. Efremov A. A., Saveleva E. E., Bulgakova N. A., Rukosueva T. V., Volkova V. A. COMPONENT COMPOSITION, ANTIRADICAL AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL OF LEMON BALM LEAVES // Chemistry of plant raw material, 2025. № 1. P. 188-196. URL: https://journal.asu.ru/cw/article/view/15329.
Issue
No 1 (2025)
Section
Low-molecular weight compounds

Copyright (c) 2025 chemistry of plant raw material

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