INDIVIDUAL VARIABILITY OF THE CONTENT OF BIOLOGICALLY ACTIVE COMPOUNDS AND IDENTIFICATION BY ISSR MARKERS IN RHODIOLA ROSEA (CRASSULACEAE) IN THE ALTAI REPUBLIC

UDC 577.13;633.8:174.015

  • Elena Viktorovna Zhmud Центральный сибирский ботанический сад СО РАН Email: elenazhmu@gmail.com
  • Olga Viktorovna Kotsupiy Central Siberian Botanical Garden SB RAS https://orcid.org/0000-0002-5326-4371 Email: olnevaster@gmail.com
  • Irina Nikolaevna Kuban Central Siberian Botanical Garden SB RAS https://orcid.org/0000-0002-4305-9729 Email: irinakuban@gmail.com
  • Altynai Alekseevna Achimova Gorno-Altai Botanical Garden – branch of the Central Siberian Botanical Garden SB RAS https://orcid.org/0000-0002-1940-2224 Email: altynay.achimova@gmail.com
  • Elena Petrovna Khramova Central Siberian Botanical Garden SB RAS https://orcid.org/0000-0002-8430-4575 Email: elenakhramova2023@yandex.ru
  • Olga Viktorovna Dorogina Central Siberian Botanical Garden SB RAS https://orcid.org/0000-0001-5729-3594 Email: olga-dorogina@yandex.ru
  • Alexander Vladimirovich Kabanov Main Botanical Garden of the Russian Academy of Sciences Email: alex.kabanow@rambler.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20250215773
Keywords: rare species, genetic diversity, gender differentiation, markers, , content of phenylpropanoids and phenylethanoids, content of phenylpropanoids and phenylethanoids, certification of highly productive forms

Abstract

Individual variability in the content of two main groups of biologically active compounds (BAC) – phenylpropanoids (PPtot) and phenylethanoids (SALtot) and the molecular genetic structure by ISSR markers in 19 individuals in the Rhodiola rosea L. cenopopulation (CP) in the Altai Republic was studied. In this CP, 11 types of spectra were identified: I (in 6 individuals), II (in 3 individuals), III (in 2 individuals) and original (in 8 individuals). In 70% of males, 7 original spectra by ISSR markers were detected. Of the 5 analyzed primers, the most informative one was UBC 857. With its primer, specific amplified DNA fragments were obtained in individuals of this species and forms with a relatively higher content of the two most significant BAC groups were certified. High content of PPtot (more than 3% in air-dried raw material) was found in 30% of male and 44% of female individuals in this CP, and SALtot (more than 0.3%, which is higher than the average value for the CP) was found in 40% of male and 70% of female individuals. Based on the obtained results, we recommend four forms of R. rosea as promising highly productive sources of biologically active substances in vitro and for cultivation in introduction. These are individuals with two types of spectra and a high content of one or both groups of substances (female) and with two unique types of spectra and a high content of PPtot+SALtot and SALtot (male).

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Author Biographies

Elena Viktorovna Zhmud , Центральный сибирский ботанический сад СО РАН

Doctor of Biological Sciences, Associate Professor, Leading Researcher of the Laboratory of Rare and Endangered Plant Species

Olga Viktorovna Kotsupiy, Central Siberian Botanical Garden SB RAS

Candidate of Biological Sciences, Senior Researcher of the Laboratory of Phytochemistry

Irina Nikolaevna Kuban, Central Siberian Botanical Garden SB RAS

Researcher of the Laboratory of Rare and Endangered Plant Species

Altynai Alekseevna Achimova, Gorno-Altai Botanical Garden – branch of the Central Siberian Botanical Garden SB RAS

Candidate of Biological Sciences, Associate Professor, Director

Elena Petrovna Khramova, Central Siberian Botanical Garden SB RAS

Doctor of Biological Sciences, Head of the Laboratory of Phytochemistry

Olga Viktorovna Dorogina, Central Siberian Botanical Garden SB RAS

Doctor of Biological Sciences, Professor, Chief Researcher, Head of the Laboratory of laboratory of rare and endangered plant species

Alexander Vladimirovich Kabanov , Main Botanical Garden of the Russian Academy of Sciences

Candidate of Biological Sciences, Senior Researcher, Head of the Laboratory of Ornamental Plants

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Published
2025-06-14
How to Cite
1. Zhmud E. V., Kotsupiy O. V., Kuban I. N., Achimova A. A., Khramova E. P., Dorogina O. V., Kabanov A. V. INDIVIDUAL VARIABILITY OF THE CONTENT OF BIOLOGICALLY ACTIVE COMPOUNDS AND IDENTIFICATION BY ISSR MARKERS IN RHODIOLA ROSEA (CRASSULACEAE) IN THE ALTAI REPUBLIC // Chemistry of plant raw material, 2025. № 2. P. 215-225. URL: https://journal.asu.ru/cw/article/view/15773.
Issue
No 2 (2025)
Section
Low-molecular weight compounds

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