ASSESSMENT OF THE CONTENT OF POLYPHENOLS IN BIOTECHNOLOGICAL RAW MATERIAL IRIS SIBIRICA L. STAR VARIETY IN COMPARISON WITH INTACT PLANTS

  • Ekaterina Alekseevna Antipova Altai State Medical University Email: lek212@mail.ru
  • Lyudmila Evdokimovna Kudrikova Altai State Medical University Email: lek212@mail.ru
  • Lyudmila Ivanovn Tikhomirova Altai State University Email: l-tichomirova@yandex.ru
  • Natalya Grigoryevna Bazarnova Altai State University Email: bazarnova@chem.asu.ru
  • Marina Yurievna Cheprasova Altai State University Email: marinacheprasova@yandex.ru
  • Elena Pavlovna Khanutova Altai State University Email: harnutova@chem.asu.ru
Keywords: I. sibirica L., secondary metabolites, regenerated plants, hydroponic plants, the intact plants, biotechnology for the production of medicinal plants

Abstract

Cultivation of plant raw materials in artificial conditions will inevitably lead to a change in the qualitative and quantitative composition of secondary metabolites in comparison with the composition of plants obtained in soil conditions. The study of the metabolic profile of such plants is very important on the one hand for our understanding of temporal (ontogenetic) and spatial changes in the metabolome of plants, on the other hand in the applied plan to obtain an alternative quality medicinal plant raw materials. In this regard, the aim of this study was to study the content of phenolic compounds in biotechnological raw materials Iris sibirica Grade Стерх in comparison with intact plants.

As objects of study used plants propagated mikrokanale (regenerants), which were further grown under conditions of hydroponics (hydroponic). Intact plants were harvested in the field at the age of six years. As a result of research in raw materials Iris sibirica Grade Стерх, grown under different conditions, were found following a group of biologically active compound (БАС), corresponding to the genus Iris L.: fenilpropionovoy acid (Komarova and ferulic acid and their derivatives), flavonoids (glycosides of apigenin, apigenin-7-O-glycoside), isoflavones, and phenolic acids (vanillic acid), neytralizuya flavonoids-glycosides (glycosides of kaempferol and apigenin), stilbene. The qualitative composition of biologically active compounds of Iris sibirica Стерх depended on the growing conditions, while the biotechnological raw materials (hydroponic plants) are the closest to intact plants in terms of the content of biologically active compounds.  This allows us to consider the biotechnology of obtaining the raw material Iris sibirica L. based on hydroponic cultivation coupled with the micropropagation in an alternative way.

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Author Biographies

Ekaterina Alekseevna Antipova, Altai State Medical University

кандидат фармацевтических наук, доцент кафедры фармации

Lyudmila Evdokimovna Kudrikova, Altai State Medical University

кандидат фармацевтических наук, доцент кафедры фармации

Lyudmila Ivanovn Tikhomirova, Altai State University

кандидат биологических наук, заведующая отделом биотехнологии растений ЮСБС АлтГУ

Natalya Grigoryevna Bazarnova, Altai State University

доктор химических наук, профессор, заведующая кафедрой органической химии, декан химического факультета

Marina Yurievna Cheprasova, Altai State University

кандидат химических наук, доцент кафедры органической химии

Elena Pavlovna Khanutova, Altai State University

кандидат химических наук, доцент кафедры физической и неорганической химии

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Хроматограмма спиртового извлечения
Published
2019-04-01
How to Cite
1. Antipova E. A., Kudrikova L. E., Tikhomirova L. I., Bazarnova N. G., Cheprasova M. Y., Khanutova E. P. ASSESSMENT OF THE CONTENT OF POLYPHENOLS IN BIOTECHNOLOGICAL RAW MATERIAL IRIS SIBIRICA L. STAR VARIETY IN COMPARISON WITH INTACT PLANTS // Chemistry of plant raw material, 2019. № 2. P. 239-250. URL: https://journal.asu.ru/cw/article/view/4799.
Section
Biotechnology