FEATURES EXTRACTS FROM BIOTECHNOLOGICAL PLANT MATERIALS IN CONNECTION WITH THE EXTRAC-TION IN SUBCRITICAL CONDITIONS AND THE DIRECTED BIOSYNTHESIS OF SECONDARY METABOLITES
Abstract
The technique of obtaining extracts in subcritical conditions requires less time and material costs, the process is more environmentally friendly, and the resulting extract has a sufficiently high quality and does not contain toxic impurities.
As a result of laboratory studies, extracts from biotechnological raw materials Potentilla longifolia, Potentilla chrisantha, Potentilla fruticosa, and Iris sibirica, extracted by water and ethyl alcohol in traditional and subcritical conditions. The amount of extractive substances in the sub-critical conditions was 1.3 times more extracted by water. The extracts found the presence of condensed and hydrolyzed tannins, xanthones, flavonoids, coumarins and other phenolic compounds, as well as alkaloids. In our experiment, anthracene derivatives were extracted only under subcritical conditions.
The developed technology for the production of medicinal plant raw materials Iris sibirica L. on the basis of hydroponics conjugated with the clonal micro-multiplication allows to increase the content of extractive substances in the regenerants by 2 times as a result of directed biosynthesis in the cultivation of 2.5 µmМ of 6-benzylaminopurine, and 1.3 times increase the content of flavonoids in the media with 7.5 µmМ of 6-benzylaminopurine. And raw materials for the production of extract I. sibirica with antiviral activity against herpes simplex virus type II should be grown on media containing 5.0-10.0 µmМ BAP + 1.0 µmM NAA + 0.1 µmM IBA.
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