THE STUDY OF INFLUENCE OF RHIZOBIUM INOCULATION ON COMPOSITION OF FREE PHENOLIC ACIDS IN ROOTS OF ETIOLATED PEA SEEDLINGS
Abstract
With a view to study the reaction of pea plants (Pisum sativum L.) by inoculation with bacteria Rhizobium in extracts from the roots of etiolated seedlings obtained in two stages (using 80% ethanol and ethyl acetate), studied composition free of phenolcarboxylic acids (PCA). The results of the research components of extracts by different methods (paper chromatography, TLC, capillary electrophoresis, UV-and GC-MS-spectroscopy, etc.) have classified as PCA in their free form p-coumaric, o-coumaric, ferulic, benzoic, p-oxybenzoic, vanillic, gallic, gentisic, salicylic acids. To him is not yet listed ς-resorcylic acid, detected for the first time in the root extracts using of GC-MS method. Monitoring the composition of CBF in 5-mm pieces of roots, conducted by the method of GC-MS analysis revealed the influence of inoculation only on the localization of gentisic acid. The possibility of joint participation of salicylic and gentisic acids in the induction of root cell responses to inoculation of roots with bacteria Rhizobium discussed.
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