SPECTROPHOTOMETRIC DETERMINATION OF THE SUM OF SAPONINS IN THE FRUIT OF THE CHINESE TREE LYCIUM CHINENSE MILL.
UDC 615.322:543.422.3
Abstract
The objects of the study were Mature dried fruits of Chinese birch (Lycium chinense Mill., this. Solanaceae (Solanaceae)). Four samples were examined: no. 1 (Gifts of Pamir, Russia), no. 2 (Gullin Tianhe Pharmaceutical, China), no. 3 (A. J. Alliance, Russia), №. 4 (Globaltorg, Russia). The purpose of this work is to determine the content of the sum of saponins in Chinese fruit trees by spectrophotometry.
By direct spectrophotometry, after reaction with concentrated sulfuric acid, the content of the sum of saponins in the raw material of Chinese wood was determined. The expediency of using the spectrophotometric method for quantitative determination of saponins in extracts from the fruit of Chinese wood is proved. The values of the optical density of the saponin solution were recorded at a wavelength of 250-500 nm. Analytical maxima of the studied compounds were determined at 325 nm typical for escin. Optimal conditions for the extraction of saponins from the raw materials of this plant (extractant – 40% ethanol; the ratio "raw material – extractant" – 1 : 50; extraction time – 90 minutes; the degree of grinding of raw materials – 0.5 mm) are justified. It was determined that the error of a single determination of the saponin content in Chinese fruit with a confidence probability of 95% is ±1.93%. It was found that the content of saponins in the studied samples of Chinese wood varies in the range of 4.94 to 5.03%.
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