EXTRACTION OF PHENOLIC COMPOUNDS AND TRITERPENES FROM INONOTUS OBLIQUUS USING A SUPERCRITICAL FLUID EXTRACTION PROCESS
UDK 544.351.3
Abstract
The supercritical fluid extraction method is effective and environmentally friendly compared to traditional methods for obtaining extracts (percolation, repercolation and others). In this work, the extraction of bioactive compounds from the mycelium of the polypore fungus (chaga, Inonotus obliquus (Ach. ex Pers.) Pil., family Hymenochaetaceae) was carried out with supercritical carbon dioxide at various parameters of pressure, temperature and with the addition of ethanol as a co-solvent, and the process was implemented extraction in a pilot extraction plant. Dependences of the extract yield at various extraction parameters on the volume of carbon dioxide passed through were revealed. It was found that with increasing extraction temperature, the yield of the resulting extract increases, but increasing the pressure does not increase the yield of the extract after 30 MPa. It has been shown that the yield of triterpenes is influenced by both the temperature of the extract and pressure. The presence of ethanol also leads to improved recovery of phenolic compounds. However, the proportion of flavonoids remains unchanged, since it is not significantly affected by changes in extraction parameters. The conducted studies showed that the data obtained at the pilot installation correspond to the data obtained at the laboratory experimental installation. This fact allows us to predict that the SCFE process, as applied to the extraction of I. obliquus, is a highly scalable process.
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