OBTAINING EXTRACT FROM GRAPE SECONDARY RAW MATERIALS
Abstract
The problem of environmental degradation, the impact on the body external adverse factors leads to the accumulation in the body of free radicals, products of incomplete reduction of oxygen, an excess of which leads to lipid peroxidation and as a consequence of dysfunction of cellular membranes. This leads to the formation of numerous diseases. Therefore, an important trend in the food industry is the production of food with high content of biologically active substances. Considered the issues of influence of technological parameters on the properties of the extract: chemical composition, antiradical, antioxidant activity, restoring force. The results of the selection of the optimal drying temperature secondary grapes, the optimal solvent, the optimum temperature of extraction, duration of extraction and the extraction process. In this paper various techniques are used to determine the level of antioxidant activity: the total content of phenolic compounds equivalent to Gallic acid, flavonoids and tannins equivalent of catechin, anthocyanins, the equivalent of cyanidin-3-glycoside, antiradical capacity using the free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) mg/ml, antioxidant activity by the method of ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mol of trolox, restoring force by the method of FRAP with 2,4,6-trierer-5-triazine, antioxidant activity in the linoleic acid system. The result revealed that the development of technology of extract from secondary grapes most significantly to polyphenol and antioxidant indexes influence the drying step of the raw material, in addition, drying allows to increase the shelf life of recycled materials, minimize waste enterprises, and to increase the content of phenolic compounds and flavonoids. It is shown that the use of certain drying temperatures of the feedstock, the duration and temperature of extraction using mixture of ethanol-water concentration under vacuum yield grape Marc extracts with high antioxidant performance.
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