THE METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN THE LEAVES OF THE TATAR MAPLE (ACER TATARICUM L.)
UDC 615.322
Abstract
Tatar maple (Acer tataricum L.) is a deciduous tree or shrub up to 8 m tall. This plant is widespread in Central, Eastern and Southeastern Europe, Asia Minor and the Caucasus. Extracts of Tatar maple have been used in traditional medicine for many years due to their antioxidant, anti-inflammatory, antidiabetic, hepatoprotective, antitumor activity, which may be due to various groups of biologically active compounds such as triterpenoids, phenol carboxylic acids and their derivatives, flavonoids, nitrogen-containing compounds. The pharmacological activity of the leaves of the Tatar maple is determined by biologically active compounds, mainly of a phenolic nature, in particular, flavonoids and derivatives of gallic acid (ginnalin A, etc.).
As a result of the conducted research, a method for quantifying the amount of flavonoids in the leaves of the Tatar maple has been developed. Differential spectrophotometry conducted in accordance with the OFS was used as a research method.1.2.1.1.0003.15 "Spectrophotometry in the ultraviolet and visible regions". The spectral characteristics of water-alcohol extracts were evaluated using a Specord 40 spectrophotometer (Analytik Jena AG, Germany).
It was determined that in all electronic spectra of extracts from the leaves of the Tatar maple, a bathochromic shift of the long-wavelength band is observed in the presence of aluminum chloride, which confirms the presence of flavonoids. Under the conditions of differential spectrophotometry, an absorption maximum is observed in the region of 412±2 nm, which indicates the expediency of using a routine having an absorption maximum at a wavelength of 412 nm in the analysis technique. The optimal parameters of extraction of raw materials were determined: extractant – 70% ethyl alcohol, the ratio "raw material-extractant" – 1 : 30, extraction time – 45 min, the degree of grinding of raw materials – 2 mm, analytical wavelength – 412 nm.
It was determined that the content of the sum of flavonoids in terms of rutin in the leaves of the Tatar maple varies from 3.18±0.05 to 3.56±0.05%. The error of a single determination with a 95% confidence probability is ± 1.47%.
The results obtained can be used in the development of a draft FS for a new type of medicinal plant raw material "Tatar Maple leaves" for introduction into the State Pharmacopoeia of the Russian Federation.
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