THE METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF HYDROXYCINNAMIC ACIDS IN BUCKWHEAT RED-STEMMED GRASS
UDC 615.322:543.422.3
Abstract
Red-stemmed buckwheat grass is registered in the territory of the Russian Federation as a biologically active food additive as a source of flavonoids. In addition to flavonoids, buckwheat raw materials contain hydroxycinnamic acids, which make a significant contribution to its pharmacological activity. Buckwheat extracts and dietary supplements based on them have antioxidant, cardioprotective, hypoglycemic, and nootropic effects. However, there is insufficient information in the domestic literature on the standardization of raw materials of red-stem buckwheat, in this regard, the purpose of this study is to identify, develop and validate a technique for the quantitative determination of hydroxycinnamic acids contained in the aboveground part of red-stem buckwheat. According to the results of qualitative identification by thin-layer chromatography, water-alcohol extracts of buckwheat grass contained hydroxycinnamic acids. The maximum absorption of extracts was observed at a wavelength of 330 nm, which corresponded to the maximum absorption of a solution of a standard sample of chlorogenic acid. Based on the data obtained, the quantitative determination of the amount of hydroxycinnamic acids was carried out in terms of chlorogenic acid. The optimal extraction parameters were determined: extractant – 40% ethyl alcohol, the ratio of "raw material - extractant" 1 : 50; extraction time is 30 minutes, the multiplicity of extraction is single. Validation of the methodology has been carried out. Four samples of red-stem buckwheat grass produced by Parafarm LLC were analyzed using the developed methodology. The content of the sum of hydroxycinnamic acids in terms of chlorogenic acid varied from 2.87±0.14 – 3.14±0.22%. The results obtained indicate that this technique can be used to standardize medicinal raw materials of red-stem buckwheat.
Downloads
Metrics
References
Fednina A.S., Makartseva M.G., Kurdyukov Ye.Ye., Moiseyeva I.Ya., Yelistratov D.G., Mitishev A.V. Voprosy bio-logicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2023, vol. 26, no. 9, pp. 27–32. https://doi.org/10.29296/25877313-2023-09-04. (in Russ.).
Gudkova A.A., Perova I.B., Eller K.I., Chistyakova A.S., Slivkin A.I., Sorokina A.A. Khimiko-farmatsevticheskiy zhurnal, 2020, vol. 54, no. 3, pp. 37–41. https://doi.org/10.30906/0023-1134-2020-54-3-37-41. (in Russ.).
Berezina Ye.V., Rybin D.A., Sukhova A.A., Somin A.A., Mishukova I.V., Brilkina A.A. Khimiya rastitel'nogo syr'ya, 2024, no. 3, pp. 129–137. https://doi.org/10.14258/jcprm.20240313979. (in Russ.).
Li J., Yang P., Yang Q. Molecules, 2019, vol. 24, 1310. https://doi.org/10.3390/molecules24071310.
Rui J., Hua-Qiang L., Chang-Ling H. International Journal of Molecular Sciences, 2016, vol. 17, 589. https://doi.org/10.3390/ijms17040589.
Hacı Y., Yabanci N., Çağdaş M. Current Nutrition & Food Science, 2020, vol. 16, pp. 29–34. https://doi.org/10.2174/1573401314666180910140021.
Subhash B., Gulab Y., Raghavendra S. Indian Journal of Agronomy, 2019, vol. 63, pp. 415–427.
Magafurova F.F., Khusnutdinov V.V. Vestnik KrasGAU, 2022, no. 9, pp. 27–32. https://doi.org/10.36718/1819-4036-2022-9-27-32. (in Russ.).
Moiseyeva I.Ya., Gizinger O.A., Mitishev A.V. Vrach, 2024, vol. 35, no. 5, pp. 37–44. https://doi.org/10.29296/25877305-2024-05-06. (in Russ.).
Kompantseva Ye.V., Saushkina A.S., Ayrapetova A.Yu. Vedomosti Nauchnogo tsentra ekspertizy sredstv med-itsinskogo primene-niya. Regulyatornyye issledovaniya i ekspertiza lekarstvennykh sredstv, 2024, vol. 14, no. 2, pp. 196–206. https://doi.org/10.30895/1991-2919-2024-14-2-196-206. (in Russ.).
Denisenko T.A., Vishnikin A.B., Tsyganok L.P. Analitika i kontrol', 2015, vol. 19, no. 4, pp. 373–380. https://doi.org/10.15826/analitika.2015.19.4.012. (in Russ.).
Sheykhmagomedova P.A., Popova O.I. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2022, vol. 25, no. 12, pp. 44–50. https://doi.org/10.29296/25877313-2022-12-07. (in Russ.).
Kurkin V.A., Ryazanova T.K. Razrabotka i registratsiya lekarstvennykh sredstv, 2022, vol. 11, no. 3, pp. 152–161. https://doi.org/10.33380/2305-2066-2022-11-3-152-161. (in Russ.).
Kompantseva Ye.V., Ayrapetova A.Yu., Saushkina A.S. Vedomosti Nauchnogo tsentra ekspertizy sredstv med-itsinskogo primeneniya. Regulyatornyye issledovaniya i ekspertiza lekarstvennykh sredstv, 2024, vol. 14, no. 2, pp. 181–195. https://doi.org/10.30895/1991-2919-2024-14-2-181-195. (in Russ.).
Sannikova Ye.G., Popova O.I., Kompantseva Ye.V., Frolova O.O. Farmatsiya i farmakologiya, 2015, vol. 3, no. 2(9), pp. 13–17. https://doi.org/10.19163/2307-9266-2015-3-2(9)-13-17. (in Russ.).
Dvornikova L.G., Turetskova V.F. Khimiya rastitel'nogo syr'ya, 2013, no. 2, pp. 127–134. (in Russ.).
Terletskaya V.A., Lukashov R.I. Sovremennyye tendentsii razvitiya tekhnologiy zdo-rov'yesberezheniya: sbornik nauchnykh trudov X Mezhdunarodnoy nauchno-prakticheskoy konferentsii molodykh uchenykh. [Modern Trends in the Development of Health-Saving Technologies: Collection of Scientific Papers of the X International Scientific and Prac-tical Conference of Young Scientists]. Moscow, 2022, pp. 290–294. https://doi.org/10.52101/9785870191058_290. (in Russ.).
Lavshuk V.V., Lukashov R.I. Vestnik Bashkirskogo gosudarstvennogo meditsinskogo universiteta, 2019, no. 4, pp. 199–203. (in Russ.).
Kurdyukov Ye.Ye., Pravdivtseva O.Ye., Semenova Ye.F. Vestnik Smolenskoy gosudarstvennoy meditsinskoy akade-mii, 2023, vol. 22, no. 2, pp. 230–234. https://doi.org/10.37903/vsgma.2023.2.31. (in Russ.).
Gosudarstvennaya farmakopeya RF. XV izd. [State Pharmacopoeia of the Russian Federation. 15th ed.]. Moscow, 2023. URL: https://pharmacopoeia.regmed.ru/pharmacopoeia/izdanie-15/?PAGEN_1=5 (in Russ.).
Copyright (c) 2025 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
