STUDY OF SEPARATE FRACTIONS OF ARISTOLOCHIA CLEMATITIS L. HERB FOR THE PRESENCE OF DIFFER-ENT GROUPS OF BAS
UDC 542.06:615.322
Abstract
The objective of the work was generation of Aristolochia clematitis L. herb fractions and their further study for the presence of different groups of biologically active substances (including aristolochic acids), which was achieved by addressing the following tasks: obtaining fractions from A. clematitis L. herb, preliminary TLC test, HPLC fraction analysis. Fractionation scheme for A. clematitis L. herb extracts was proposed. 4 fractions with different distribution of biologically active substances (chloroform, butanol, ethylacetate and water) were obtained. Using the TLC method and HPLC analysis the aristolochic acids were detected in the chloroform fraction only, suggesting that chloroform is a selective extractant for aristolochic acids. Presence of hydroxycinnamic acids in fractions was defined by HPLC method. Trace quantities of cinnamic acid were found in all fractions, with the highest content noted in the chloroform fraction. Caffeic acid is seen in all fractions, the highest content of butanol is typical of butanol fraction. Chlorogenic acid is present in almost all fractions, its basic amount accounted for 96% ethanol sub-fraction of ethyl-acetate fraction and 20% ethanol sub-fraction of butanol fraction. Some nitrogen-containing substances were identified in ethyl-acetate fraction, presumably of alkaloid type, but not the aristolochic acids, which makes possible further study of ethyl-acetate extracts.
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