PREPARATION OF MEDICINAL PLANT RAW MATERIALS WITH A GIVEN CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY
UDC 615.322:582. 579.2:581.192
Abstract
A significant effect on the amount of hydroxycinnamic acids was not an increase in the content of bap from BA 1.0 to 10.0 μM, but the introduction of auxins of NAA 1.0 μM + IBA 0.1 μM. Auxins in combination with cytokinin BA increased the amount of acids by an average of 20% in alcoholic (1.9±0.2%) and aqueous (1.46±0.06%) extracts from Iris sibirica raw materials. The maximum yield of extractive substances, including tannins (4.4±0.3%) was observed when growing raw materials on media with a BA content of 2.5–5.0 μM, the lowest value was obtained with a BA of 10.0 μM. The introduction of auxins (NAA 1.0 μM + IBA 0.1 μM) did not significantly affect the dynamics of the accumulation of tannins.
Screening of the chemical composition of medicinal plant raw materials Origanum vulgare and Salvia officinalis obtained by biotechnology methods showed the presence of flavonoids of 1.6±0.1% and 3.2±0.04%, hydroxycinnamic acids of 7.4% and 13.4% at a.s.s., respectively.
Antibacterial activity with a minimum inhibitory concentration of 0.02-0.04 mg/ml against Sporosarcina ureae, Bacillus pumilus, Salmonella typhimurium, Staphylococcus aureus was pronounced in the hexane extract of Salvia officinalis. Ethanol extract of sage was active against Salmonella typhimurium. Ethanol extracts of Iris sibirica and Salvia officinalis showed antibacterial activity against Salmonella typhimurium, MIC-0.35 and 0.16 mg / ml, respectively. Siberian iris suppressed Bacillus pumilus at MIC-0.7 mg / ml. The largest amount of extractive substances was extracted from the raw materials of Origanum vulgare with an aqueous-glycerol mixture – 21.5±0.2 % per a. s. s. Against Salmonella typhimurium, the extracts showed antibiotic activity with MIC – 1.2 mg/ml.
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Savchenko I.V., Zayko L.N., Khaziyeva F.M., Tsitsilin A.N. Maslyakov V.Yu. Zernobobovyye i krupyanyye kul'tury, 2016, no. 2(18), pp. 32–36. (in Russ.).
Medicinal Plants Industry 2017. URL: http://tejasisblog.blogspot.ru/2017/05/medicinal-plants-industry-2017.html
Food and Agriculture Organization of the United Nations. URL: http://www.fao.org/biodiversity/2010-international-year-of-biodiversity/en/
Crozier A., Jaganath I.B., Clifford M.N. Plant Second Metab Occur Struct Role Hum Diet., 2007, pp. 1–24. DOI: 10.1002/9780470988558.ch1.
González-Lamothe R., Gabriel M., Mariza G., Moussa S.D., François M., Kamal B. Int. J. Mol. Sci., 2009, vol. 10, pp. 3400–3419. DOI: 10.3390/ijms10083400.
Radulovic N.S., Blagojevic P.D., Stojanovic-Radic Z.Z., Stojanovic N.M. Curr. Med. Chem., 2013, vol. 20, pp. 932–952.
Budanova Ye.V., Gorlenko K.L., Kiselev G.Yu. Antibiotiki i khimioterapiya, 2019, vol. 64, pp. 5–6. DOI: 10.24411/0235-2990-2019-100034. (in Russ.).
Garvey M.I., Rahman M.M., Gibbons S., Piddock L.J.V. J. Antimicrob. Agents, 2010, vol. 37 (2), pp. 145–151. DOI: 10.1016/j.ijantimicag.2010.10.027.
Paiva P.M.G., Gomes F.S., Napoleão Th. Curr. Res. Technol. Educ. Top Appl. Microbiol. Microb. Biotechnol., 2010, pp. 396–406.
Borges A., Ferreira C., Saavedra M.J., Simões M. Microb Drug Resist., 2013, vol. 19, pp. 256–265. DOI: 10.1089/mdr.2012.0244.
Omojate G.C., Enwa F.O., Jewo A.O., Eze C.O. J. Pharm. Chem. Biol. Sci., 2014, vol. 2, pp. 77–85.
Roger T., Pierre-Marie M., Igor V., Patrick V. J. Appl. Pharm. Sci., 2015, vol. 5 (06), pp. 034–049. DOI: 10.7324/JAPS.2015.50606.
Zilfikarov I.N., Zhilin A.V. Farmatsiya, 2007, no. 2, pp. 7–9. (in Russ.).
Kalinin F.L., Sariatskaya V.V., Polishchuk V.Ye. Metody kul'tury tkaney v fiziologii i biokhimii rasteniy. [Tissue culture methods in plant physiology and biochemistry]. Kiev, 1980, 488 p. (in Russ.).
Murashige T., Skoog F. Physiol. Plant., 1962, vol. 15, no. 4, p. 473.
Tikhomirova L.I., Bazarnova N.G., Il'icheva T.N., Martirosyan Yu.Ts., Sinitsyna A.A. Khimiya rastitel'nogo syr'ya, 2018, no. 4, pp. 235–245. DOI: 10.14258/jcprm.2018043887. (in Russ.).
Tikhomirova L.I., Shcherbakova L.V., Ponomarova Ya.V., Drobysheva Ye.A., Chukubayeva A.N. Khimiya rastitel'nogo syr'ya, 2019, no. 4, pp. 315–326. DOI: 10.14258/jcprm.2019046322. (in Russ.).
Muzychkina R.A. Tekhnologiya proizvodstva i analiz fitopreparatov. [Production technology and analysis of phyto-preparations]. Almaty, 2011, 360 p. (in Russ.).
Gulyayev D.K., Belonogova V.D. Mashchenko P.S. Vestnik VGU, seriya: khimiya, biologiya, farmatsiya, 2019, no. 2, pp. 80–86. (in Russ.).
Opredeleniye chuvstvitel'nosti mikroorganizmov k antibakterial'nym preparatam: metodicheskiye ukazaniya. [Determi-nation of the sensitivity of microorganisms to antibacterial drugs: Guidelines]. Moscow, 2004, 91 p. (in Russ.).
Pierozan M.K., Pauletti G.F., Rota L., Santos A.S.A., Lerin L., Di Luccio M., Mossi A.J., Atti-serafini. L., Can-sian R.L., Oliveira J.A. Ciência e Tecnologia de Alimentos, 2009, vol. 29(4), pp. 764–770.
Khaziyev R.Sh., Petrova D.N., Sitenkov A.Yu. Butlerovskiye soobshcheniya, 2015, vol. 41, no. 3, pp. 115–118. (in Russ.).
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