DEVELOPMENT OF METHODS OF QUALITY CONTROL OF GINSENG TINCTURA
Abstract
There were developed the techniques of qualitative and quantitative analysis of ginseng tinctures (Panax ginseng C.A. Meyer).
Thin-layer chromatography (in the solvent system chloroform – methanol – water, 26 : 14 : 3; detection in the visible range after reaction with phosphowolframic acid) and UV/Visible spectroscopy of native tincture (maximum of absorption at 268±2 nm) and total saponins, purified on polyamide and reacted with 70% sulphuriс acid (maximum of absorption at 280±2 nm, 400±2 nm and 526±2 nm).
The method of quantitative determination of total saponins in tinctura of ginseng by means of direct spectrophotometry (analytical wave-length at 526 nm) was developed. The contents of the total saponins are varied with 0,42% to 0,61% (calculated on ginsenoside Rg1)Downloads
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