STRUCTURAL AND CHEMICAL CHARACTERISTIC OF PECTIN FROM PICEA ABIES GREENERY
UDC 547.458:547.458.87:547.458.88
Abstract
The present work aimed to determine structural features of polysaccharides derived from the P. abies foliage by extraction with a (NH4)2C2O4 solution. The isolated polysaccharide was studied in detail by the methods of ion exchange chromatography, partial acidic hydrolys and NMR spectroscopy. It was shown that this polysaccharide contained polymers of various structures. The major constituents of PAO were low-methoxyl and low-acetylated 1,4-a-D-galacturonan and by minor parts of partly 2-O- and/or 3-O- acetylated rhamnogalacturonan-I (RG-I). The side carbohydrate chains of the branched region of RG-I were represented predominantly by highly branched 1,5-a-L-arabinan and minor portions of 1,4-β-D-galactan. In addition to the dominant pectins, polysaccharide PAO contained binding glycans of the glucomannans class, which indicated a close interaction of these polysaccharides in the cell walls. Thus, the structural features of pectin woody P. abies, extracted with a solution of (NH4)2C2O4, were first determined. It can be concluded that P. abies woody greens, a large tonnage waste from the wood processing industry, can be considered as a potential source of pectin substances. The results of studying the structure of components of woody green P. abies can be the basis for the development and improvement of new technologies for the integrated use of this raw material.
Downloads
References
Harholt J., Suttangkakul A., Scheller H.V. Plant physiology, 2010, vol. 153, pp. 384–395. DOI: 10.1104/pp.110.156588.
Caffall K.H., Mohnen D. Carbohydrate Research, 2009, vol. 344, pp. 1879–1900. DOI: 10.1016/j.carres.2009.05.021.
Voragen A.G., Coenen G.J., Verhoef R.P., Schols H.A. Structural Chemistry, 2009, vol. 20, pp. 263–275.
Butylkina A.I., Levdanskiy V.A., Kuznetsov B.N. Khimiya Rastitel'nogo Syr'ya, 2011, no. 2, pp. 77–82. (in Russ.).
Medvedeva Ye.N., Babkin V.A., Ostroukhova L.A. Khimiya Rastitel'nogo Syr'ya, 2003, no. 1, pp. 27–27. (in Russ.).
Ivanova N.V., Popova O.V., Babkin V.A. Khimiya Rastitel'nogo Syr'ya, 2003, no. 4, pp. 5–7. (in Russ.).
Yefremov A.A., Kondratyuk T.A. Khimiya Rastitel'nogo Syr'ya, 2008, no. 4, pp. 171–176. (in Russ.).
Ivanova N.V., Ovodova R.G., Babkin V.A. Khimiya Rastitel'nogo Syr'ya, 2006, no. 1, pp. 15–20. (in Russ.).
Fyhrquist P., Virjamo V., Hiltunen E., Julkunen-Tiitto R. Fitotherapia, 2017, vol. 117, pp. 138–146. DOI: 10.1016/j.fitote.2017.01.011.
Le Normand M., Mélida H., Holmbom B., Michaelsen T.E., Inngjerdingen M., Bulone V., Paulsen B.S., Ek M. Car-bohydrate Polymers, 2014, vol. 101, pp. 699–704. DOI: 10.1016/j.carbpol.2013.09.067.
Makarova E.N., Shakhmatov E.G., Belyy V.A. Carbohydrate Polymers, 2017, vol. 157, pp. 686–694. DOI: 10.1016/j.carbpol.2016.10.035.
Makarova E.N., Shakhmatov E.G., Belyy V.A. Carbohydrate Polymers, 2018, vol. 195, pp. 207–217. DOI: 10.1016/j.carbpol.2018.04.074.
Shakhmatov E.G., Belyy V.A., Makarova E.N. Carbohydrate Polymers, 2018, vol. 199, pp. 320–330. DOI: 10.1016/j.carbpol.2018.07.027.
Dubois M., Gilles K.A., Hamilton J.K., Rebers P.T., Smith F. Analytical chemistry, 1956, vol. 28, pp. 350–356.
Usov A.I., Bilan M.I., Klochkova N.G. Botanica marina, 1995, vol. 38, pp. 43–52. DOI: 10.1515/botm.1995.38.1-6.43.
Bradford M.M. Analytical Biochemistry, 1976, vol. 72, pp. 248–254. DOI: 10.1016/0003-2697(76)90527-3.
Wood P.J., Siddiqui I.R. Analytical Biochemistry, 1971, vol. 39, pp. 418–423. DOI: 10.1016/0003-2697(71)90432-5.
Bédouet L., Courtois B., Courtois J. Carbohydrate Research, 2003, vol. 338, pp. 379–383. DOI: 10.1016/S0008-6215(02)00500-1.
Prabasari I., Pettolino F., Liao M.L., Bacic A. Carbohydrate Polymers, 2011, vol. 84, pp. 484–494. DOI: 10.1016/j.carbpol.2010.12.012.
Salato G.S., Ponce N M., Raffo M.D., Vicente A.R., Stortz C.A. Postharvest biology and technology, 2013, vol. 84, pp. 66–73. DOI: 10.1016/j.postharvbio.2013.04.009.
Taboada E., Fisher P., Jara R., Zúñiga E., Gidekel M., Cabrera J.C., Pereira E., Gutiérrez-Moraga A., Villalonga R., Cabrera G. Food Chemistry, 2010, vol. 123, pp. 669–678. DOI: 10.1016/j.foodchem.2010.05.030.
Willför S., Holmbom B. Wood Science and Technology, 2004, vol. 38, pp. 173–179.
Cardoso S.M., Ferreira J.A., Mafra I., Silva A.M., Coimbra M.A. Journal of Agricultural and Food Chemistry, 2007, vol. 55, pp. 7124–7130. DOI: 10.1021/jf070769.
Westphal Y., Kühnel S., Waard P., Hinz S.W.A., Schols H.A., Voragen A.G.J., Gruppen H. Carbohydrate Research, 2010, vol. 345, pp. 1180–1189. DOI: 10.1016/j.carres.2010.03.042.
Yapo B.M. Polymer Reviews, 2011, vol. 51, pp. 391–413. DOI: 10.1080/15583724.2011.615962.
Hannuksela T., Penhoat C.H. Carbohydrate Research, 2004, vol. 339, pp. 301–312. DOI: 10.1016/j.carres.2003.10.025.
Femenia A., Rigby N.M., Selvendran R.R., Waldron K.W. Carbohydrate Polymers, 1999, vol. 39, pp. 151–164. DOI: 10.1016/S0144-8617(99)00003-X.
Košťálová Z., Hromádková. Z., Berit. S.P., Ebringerová A. Carbohydrate Research, 2014, vol. 398, pp. 19–24. DOI: 10.1016/j.carres.2014.08.009.
Park Y.B., Cosgrove D.J. Plant and Cell Physiology, 2015, vol. 56, pp. 180–194. DOI: 10.1093/pcp/pcu204.
Cornuault V., Posé S., Knox J.P. Food Chemistry, 2018, vol. 246, pp. 275–285. DOI: 10.1016/j.foodchem.2017.11.025.
Perrone P., Hewage C.M., Thomson A.R., Bailey K., Sadler I.H., Fry S.C. Phytochemistry, 2002, vol. 60, pp. 67–77. DOI: 10.1016/S0031-9422(02)00039-0.
Petersen B.O., Meier S., Duus J.Ø., Clausen M.H. Carbohydrate Research, 2008, vol. 343, pp. 2830–2833. DOI: 10.1016/j.carres.2008.08.016.
Copyright (c) 2020 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
