WATER-SOLUBLE POLYSACCHARIDES OF HERBS OF SUCKLING SEEDING MEDICAGO SATIVA (FABACEAE) FLORA OF THE KRASNOYARSK TERRITORY
Abstract
Alfalfa is a very high-quality perennial leguminous grass and has a long history of cultivation and application in traditional medicine in Russia. Alfalfa polysaccharides was extracted from alfalfa stems and leaves with distilled water in proportion to 1 : 20 and was characterized with different methods. The uronic acid content of the polysaccharides was quantificated colorimetrically with modified carbazole – sulfuric method – 13,1 ± 0,9%. The protein content of the polysaccharides was quantificated with Lowry protein assay – 4,25 ± 0,28%. The nucleinic acids contents was quantificated with Spirin's method – 0,0056 ± 0,0003%. Ash-content (mineral impurities) was quantificated with gravimetrical method – 3,45 ± 0,18%. The description of IR spectrum was given. The monosaccharide composition of alfalfa polysaccharides was analysed by gas chromatography-mass spectrometry. The molecular weight was determined by high performance size-exclusion liquid chromatography. The crude alfalfa polysaccharides were fractionated by DEAE-cellulose chromatography. The sugar content of the fractions was assayed using the carbazole-sulphuric acid method. The alfalfa polysaccharides represents mix of seven polysaccharides.
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