LDI MASS-SPECTROMETRY ASSESSMENT OF HYPERICIN, HYPERFORIN AND PSEUDOHYPERICIN IN CULTIVAR AND WILD HYPERICUM PERFORATUM L. IN VIVO AND IN VITRO
Abstract
The abilities to form hypericin, pseudohypericin and hyperforin in vivo Hypericum perforatum L. and in vitro by microclonal propagation were investigated. Wild and cultural (cultivar Solnechney) plants were used as the research objects. The aerial parts of the plants, which had been obtained in vivo, as well as the leaves, stems and plant callus tissue which had been obtained in vitro, were freeze-dried. Before the start of measurement methanol extraction was being carried out for 1 hour. TOF mass spectrometer with laser desorption/ionization (LDI) were used for detecting certain chemical compounds. All research chemicals were found in the methanol extract of freeze-dried lyophilized wild and cultivated plants, grown in vivo. All of the mentioned compounds were also detected in vitro in wild grown Hypericum perforatum L, however mass spectrometry couldn’t detect hyperforin in cultivar Solnechney plants. After freeze-dried lyophilized plants storing in desiccator in the dark place at 20 °С for 2,5 month all compounds were found only in vivo grown plants. In plants of wild and cultivated Hypericum grown in vitro only pseudohypericin was detected.Downloads
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